• AARTHI M Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • KAMALANATHAN D Department of Biotechnology, K. S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Tamil Nadu, India.
  • BALAKRISHNAN V Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India.


Objective: The aim of the present investigation is to isolate actinomycetes from the sediments of Pichavaram mangrove forest, South India, and to screen for their antibacterial efficiency.

Methods: Actinomycetes were isolated by culturing the samples in Starch Casein Agar medium; they were screened primarily for their antibacterial efficiency against Gram-positive and Gram-negative bacterial organisms. Solvent extraction was done with 50% (percentage) ethyl acetate, crude extracts of actinomycetes were prepared at different concentrations using dimethyl sulfoxide and treated against the bacterial organisms. Antibacterial assay was done in Mueller–Hinton agar medium.

Results: Thirteen actinomycetes were isolated; among them, four actinomycete isolates (Pichavaram mangrove actinomycete 2 [PMA2], PMA6, PMA9, and PMA13) exhibited antibacterial activity.

Conclusion: Isolate PMA2 exhibited very strong antibacterial activity and isolate PMA13 is weakly active against the tested bacterial organisms.

Keywords: Actinomycetes, Starch casein agar medium, Salinity, Crude extract, Antibacterial efficiency, Antibacterial assay


1. Ganesan P, Reegan AD, David RH, Gandhi MR, Paulraj MG, Al-Dhabi NA, et al. Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India. Alex J Med 2017;53:101-10.
2. Elbendary AA, Hessain AM, El-Hariri MD, Seida AA, Moussa IM, Mubarak AS, et al. Isolation of antimicrobial producing Actinobacteria from soil samples. Saudi J Biol Sci 2018;25:44-6.
3. Benhadj M, Gacemi-Kirane D, Menasria T, Guebla K, Ahmane Z. Screening of rare actinomycetes isolated from natural wetland ecosystem (Fetzara Lake, Northeastern Algeria) for hydrolytic enzymes and antimicrobial activities. J King Saud Univ Sci 2019;31:706-12.
4. Manilal A, Sujith S, Selvin J, Kiran GS. Antibacterial activity of Falkenbergia hillebrandii (born) from the Indian coast against human pathogens. Phyton 2009;78:161-6.
5. Freeman BC, Beattie GA. An overview of plant defenses against pathogens and herbivores. Plant Health Instruct 2008. DOI: 10.1094/PHI-I-2008-0226-01.
6. Suresh M, Mohammad SA, Pradipta KR, Panneerselvam A, Thajuddin N. Screening and antibacterial efficacy of selected Indian medicinal plants. Asian Pac J Trop Biomed 2016;6:185-91.
7. Kumar PS, Duraipandiyan V, Ignacimuthu S. Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA7. Kaohsiung J Med Sci 2014;30:435-46.
8. Aarthi M, Eswaran P, Rajeshkumar MP, Balakrishnan V. Isolation and characterization of gelatinase producing halophilic Bacteria from the sediments of pichavaram mangrove forest, Tamil Nadu state, South India. Res Rev 2019;9:63-70.
9. Zothan P, Passari AK, Chandra P, Leo VV, Mishra VK, Kumar B, et al. Production of potent antimicrobial compounds from Streptomyces cyaneofuscatus associated with fresh water sediment. Front Microbiol 2017;8:68.
10. Stephenson FH. Calculations for Molecular Biology and Biotechnology, A Guide to Mathematics in the Laboratory. 2nd ed. United States: Elsevier Inc., Academic Press; 2010.
11. Rao AS, Shobha KL, Shetty MS, Pai KS. In vitro antibacterial and antifungal activities of aqueous and ethanolic leaf extracts of Acacia auriculiformis. Asian J Pharm Clin Res 2018;11:480-2.
12. Akhtar MS, Hossain MA, Said SA. Isolation and characterization of antimicrobial compound from the stem bark of the traditionally used medicinal plant Adenium obesum. J Tradit Complement Med 2017;7:296-300.
13. Mathur N, Paliwal A, Sharma P, Kumar M, Bhatnagar P. Antimicrobial compound from Streptomyces isolate characterized using HPLC. Univ J Environ Res Technol 2012;4:242-6.
14. Jayasree P, Thiruchelvi R, Balashanmugam P. Evaluation of antibacterial, antioxidant and anticancer potentials from marine red algae Gracilaria corticata. Asian J Pharm Clin Res 2018;11:347-50.
15. Ram D, Devang P. Screening and characteristic study of antimicrobial actinomycetes from nearby soil of medicinal plants. Int J Pharm Pharm Sci 2018;10:66-73.
16. Kumar C, Sharmila KJ. Comparative study of wound healing activity of Indian medicinal plant and herb against diabetic foot ulcer (Piper nigrum and Arachis hypogaea). Asian J Pharm Clin Res 2019;12:35-9.
17. Sudha SK, Hemalatha R. Isolation and screening of antibiotic producing actinomycetes from garden soil of Sathyabama University, Chennai. Asian J Pharm Clin Res 2015;6:110-4.
18. Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolics content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 2009;22:102-6.
19. Chopra RN, Nayer SL, Chopra IC. Glossary of Indian Medicinal Plants. 3rd ed. New Delhi: Council of Scientific and Industrial Research; 1992.
20. Bruneton J. Pharmacognosy Phytochemistry Medicinal Plants. France: Lavoisier Publishing Co.; 1995.
21. Khalil MY, Moustafa AA, Naguib NY. Growth, phenolic compounds and antioxidant activity of some medicinal plants grown under organic farming condition. World J Agric Sci 2007;3:451-7.
22. Dushyant S, Reena R, Monika C, Yadav JP. Antibacterial capacity and identification of bioactive compounds by GCMS of Allium cepa. Int J Pharm Pharm Sci 2018;10:116-21.
23. Idrus HH, Hatta M, Febriza A, Kasim VN. Antibacterial activities of Sapodilla fruit extract inhibiting Salmonella typhi on mice BalB/C. Int J Appl Pharm 2019;11:121-6.
24. Islam MS, Islam MS, Hossain MA, Ahmed S, Aktaruzzaman M, Akanda MR, et al. In vitro antibacterial activity of medicinal plants turmeric, chinnamon and clove against GM (+ve) and GM (-ve) Bacteria. Int J Curr Pharm Res 2019;11:85-8.
25. Rahman A, Islam Z, Islam A. Antibacterial activities of actinomycete isolates collected from soils of Rajshahi, Bangladesh. Biotechnol Res Int 2011;2011:1-6.
26. Arifuzzaman M, Khatun MR, Rahman H. Isolation and screening of actinomycetes from Sundarbans soil for antibacterial activity. Afr J Biotechnol 2010;9:4615-9.
27. MPEDA, NACA. Shrimp Health Management Extension Manual. Kochi, India: MPEDA, NACA; 2003.
28. Wahyudi AT, Priyanto JA, Wulandari DR, Astuti RI. In vitro antibacterial activities of Marine sponge associated Bacteria against pathogenic Vibrio spp causes vibriosis in shrimps. Int J Pharm Pharm Sci 2019;11:33-7.
29. Mithilesh S, Neha P, Vasudha A, Singh KK, Anitha P. Antioxidant, antimicrobial activity and bioactive compounds of Bergenia ciliate Sternb: A valuable medicinal herb of Sikkim Himalaya. J Tradit Complement Med 2017;7:152-7.
30. Deependra T, Kamana S. In vitro antimicrobial activity and MIC of the extracellular ethylacetate crude extract of endophytic fungi Fusarium sp. isolated from Tephrosia purpurea root. Int J Pharm Pharm Sci 2019;11:48-53.
31. Bushra AK, Al-Mahbashi HM, Riyadh SA, Faten AA. Phytochemical, anti-inflammatory, antioxidant, cytotoxic and antibacterial study of Capparis cartilaginea Decne from Yemen. Int J Pharm Pharm Sci 2018;10:38-44.
32. Tripathi KD. Essential Medical Pharmacology. 6th ed. New Delhi: Jaypee Brothers Medical Publishers Ltd.; 2008.
33. Shobha KL, Rao AS, Pai KS, Sujatha B. Antimicrobial activity of aqueous and ethanolic leaf extracts of Anacardium occidentale. Asian J Pharm Clin Res 2018;11:474-6.
34. Baruah IC, Barua M. Non-conventional food plants of medicinal use in Northeast India. In: Borthakur SK, Ahmed A, Gogoi P, Dutta DN, Ahmed GU, editors. Medicinal Plants of Northeast India. Guwahati, Assam: North Eastern Development Finance Corporation Ltd.; 2010.
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How to Cite
M, A., K. D, and B. V. “ISOLATION OF ACTINOMYCETES FROM THE SEDIMENTS OF PICHAVARAM MANGROVE FOREST, SOUTH INDIA AND ANALYSING THEIR ANTIBACTERIAL EFFICACY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 4, Feb. 2020, pp. 120-5, doi:10.22159/ajpcr.2020.v13i4.36702.
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