ISOLATION OF ACTINOMYCETES FROM THE SEDIMENTS OF PICHAVARAM MANGROVE FOREST, SOUTH INDIA AND ANALYSING THEIR ANTIBACTERIAL EFFICACY

  • AARTHI M Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • KAMALANATHAN D Department of Biotechnology, K. S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Tamil Nadu, India.
  • BALAKRISHNAN V Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India.

Abstract

Objective: The aim of the present investigation is to isolate actinomycetes from the sediments of Pichavaram mangrove forest, South India, and to screen for their antibacterial efficiency.


Methods: Actinomycetes were isolated by culturing the samples in Starch Casein Agar medium; they were screened primarily for their antibacterial efficiency against Gram-positive and Gram-negative bacterial organisms. Solvent extraction was done with 50% (percentage) ethyl acetate, crude extracts of actinomycetes were prepared at different concentrations using dimethyl sulfoxide and treated against the bacterial organisms. Antibacterial assay was done in Mueller–Hinton agar medium.


Results: Thirteen actinomycetes were isolated; among them, four actinomycete isolates (Pichavaram mangrove actinomycete 2 [PMA2], PMA6, PMA9, and PMA13) exhibited antibacterial activity.


Conclusion: Isolate PMA2 exhibited very strong antibacterial activity and isolate PMA13 is weakly active against the tested bacterial organisms.

Keywords: Actinomycetes, Starch casein agar medium, Salinity, Crude extract, Antibacterial efficiency, Antibacterial assay

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M, A., K. D, and B. V. “ISOLATION OF ACTINOMYCETES FROM THE SEDIMENTS OF PICHAVARAM MANGROVE FOREST, SOUTH INDIA AND ANALYSING THEIR ANTIBACTERIAL EFFICACY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 4, Feb. 2020, pp. 120-5, doi:10.22159/ajpcr.2020.v13i4.36702.
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