• SAMAR RAJENDRA KUMAR Department of Medicine, Pacific Institute of Medical Sciences, Udaipur, Rajasthan, India.
  • JAIN SUMAN Department of Biochemistry, Pacific Institute of Medical Sciences, Udaipur, Rajasthan, India.
  • SOGANI SONAL Department of Biochemistry, Pacific Institute of Medical Sciences, Udaipur, Rajasthan, India.


Objective: Liver cancer is the leading cause of death due to malignancies all over the world. Free radical-induced oxidative stress extent can be provoked by antioxidant mechanisms decreased efficiency. The present study was carried out to investigate the extent of oxidative stress and the levels of antioxidants in the circulation of patients with liver cancer.

Methods: Plasma thiobarbituric acid reactive substances (TBARS) and level of some antioxidant enzymes as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and GSH be determined in the circulations of 100 Indian and Hungarian patients with liver cancer and an equal number of age-matched normal subjects.

Results: Significantly increased concentrations of TBARS plasma levels and significantly lowered levels of SOD, CAT, GSH, and GSH-Px were observed in patients with liver cancer which may have occurred due to increased use to scavenge lipids peroxides as well as their sequestration by tumor cells. Increased lipid peroxidation in liver was seen which may be due to excessive oxidative stress. Comparison between Indians and Hungarians subjects revealed 200% increased malondialdehyde levels in Hungarian male patients as compared to Indian male patients. SOD was found to decrease in Indian subjects whereas CAT (20%) higher, GSH (25–35%) less decrease GSH-Px (5%) more decrease in Hungarians patients compared to Indian patients.

Keywords: Antioxidants, Peroxidative stress, Malondialdehyde (MDA), Superoxide Dismutase (SOD), Catalase, Glutathione (GSH) and Glutathine peroxidase (GSH-PX)


1. Perin N. Global variation in cancer incidence and mortality. Curr Sci 2001;l81:465.
2. New Hope for Liver Cancer Patients. 6th ed. Newstract India; 2007.
3. Khanna K, Nikunji N, Khanna HD. A study of antioxidant and their preventive potential in cervical Dysplasia. Asian J Obs Gynae Pract 2002;l6:3-11.
4. Diplock AT. Antioxidant nutrients and disease prevention: An overview. Am J Clin Nutr 1991;53:189S-193S.
5. Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicines. 3rd ed. UK: Oxford Science Publications; 1990. p. 192.
6. Rajneesh CP, Manimaran A, Sasikala KR, Adaikappan P. Lipid peroxidation and antioxidant status in patients with breast cancer. Singapore Med J 2008;49:640-3.
7. Lopaczynski W, Zeisel SH. Antioxidants: Automatic cell death. Cancer Nutr Res 2001;21:295-307.
8. Parola M, Leonarduzzi G, Robino G, Albano E, Poli G, Dianzani MU, et al. On the role of lipid peroxidation in the pathogenesis of liver damage induced by long-standing cholestasis. Free Radic Biol Med 1996;20:351-9.
9. Yagi K. Lipid peroxides and human diseases. Chem Phys Lipids 1987;45:337-51.
10. Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130-2.
11. Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.
12. Fink R, Clemens MR, Dormandy TL. Increased free radical activity in Alcoholics. Lancet 1985;2:291.
13. Lecomte E, Herberth B, Pirollet P, Chancerelle Y, Arnaud J. Effect of alcohol consumption on blood antioxidant nutrients and oxidative stress indicators. Am J Clin Nutr 1994;60:255.
14. Singh G. Assessment of Nephrotoxicity in Health and Diseases with Special Reference to Environment. Udaipur: Ph. D Thesis Submitted to MLS University; 1997.
15. Green EL, Paller SM. Oxy free radicals in acute renal failure. Miner Electrolyte Metab 1991;17:124.
16. Jacob RA, Burri BJ. Oxidative damage and defense. Am J Clin Nutr 1996;l63:985.
17. Esra B, Umit MS, Cansin S, Serpil E, Omer K. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9-19.
18. Malgorzata N, Andrzej G. The role of the reactive oxygen species and oxidative stress in the pathomechanism of age related ocular disease and other pathologies of anterior and posterior eye segments in adults. Oxid Med Cell Longev 2016;2016:3164734.
19. Santos JM, Tewari S, Goldberg AF, Kowluru RA. Mitochondrial biogenesis and the development of diabetic retinopathy. Free Radic Biol Med 2011;51:1849-60.
20. Stadtman ER. Role of oxidant species in aging. Curr Med Chem 2004;11:1105-12.
21. Tung KH, Wilkens LR, Wu AH, McDuffie K, Hankin JH, Nomura AM, et al. Association of dietary Vitamin A, carotenoids, and other antioxidants with the risk of ovarian cancer. Cancer Epidemiol Biomarkers Prev 2005;143:669-76.
22. Kurahashi N, Inoue M, Iwasaki M, Tanaka Y, Mizokami M, Tsugane S. Vegetable, fruit and antioxidant nutrient consumption and subsequent risk of hepatocellular carcinoma: A prospective cohort study in Japan. Br J Cancer 2009;100:181-4.
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How to Cite
KUMAR, S. R., J. SUMAN, and S. SONAL. “CLINICAL STUDIES TO EVALUATE PEROXIDATIVE STRESS AND ANTIOXIDANT COMPLEMENT IN LIVER CANCER IN INDIAN AND HUNGARIAN SUBJECTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 3, Mar. 2020, pp. 95-97, doi:10.22159/ajpcr.2020.v13i3.36710.
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