PHYTOCHEMISTRY SCREENING AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS PRESENT IN KARO TRADITIONAL OIL, AN INDONESIAN TRADITIONAL HERBAL MEDICINE

NOVA PRIMADINA; ACHMAD BASORI; DAVID S PERDANAKUSUMA

doi:10.22159/ajpcr.2020.v13i2.36736

NOVA PRIMADINA Department of Airlangga University, Surabaya, Indonesia.
ACHMAD BASORI Department of Pharmacology, Airlangga University, Surabaya, Indonesia.
DAVID S PERDANAKUSUMA Department of Plastic Surgery, Airlangga University, Dr. Soetomo Hospital, Surabaya, Indonesia.

DOI https://doi.org/10.22159/ajpcr.2020.v13i2.36736

Abstract

Objective: The purpose of this research was to observe the bioactive compounds of Karo traditional oil by phytochemistry screening and gas chromatography-mass spectrometry (GC-MS) analysis. Alkaloid,

Results: Terpenoid and flavonoid compounds were found, and no polyphenol compound from phytochemistry screening and 16 constituents were identified from the GC-MS analysis, α-pinene was found as the major component (74.47%), delta-3-carene (9.62%), 1% of octane, dodecane, camphor, undecane, isodecane, sabinene, hexadecane, nonane, and <1% tridecane, cyclohexane, pentacosane, heptadecan, limonene, and camphene.

Conclusion: Karo traditional oil works as a potent anti-inflammation at the inflammation phase of the wound healing process by suppressing the pro-inflammatory cytokines and promote anti-inflammatory cytokines.

Keywords: Traditional medicine, Indonesia traditional medicine, Phytochemistry screening, Gas chromatography-mass spectrometry, Karo oil, Karo traditional oil, Wound healing

Author Biography

DAVID S PERDANAKUSUMA, Department of Plastic Surgery, Airlangga University, Dr. Soetomo Hospital, Surabaya, Indonesia.

Lecturer of Plastic Surgery Department of Airlangga University - dr.Soetomo Hospital Surabaya – Indonesia
Vice dean of Medical Faculty, Airlangga University

References

1. Utami UD. Comparison of quality of karo oil produced by CV. In: Fruits and Refining Methods. Medan: North Sumatera University; 2018.
2. Soni H, Singhai AK. A recent update of botanical for wound healing activity. Int Res J Pharm 2012;3:1-7.
3. Elfahmi, Woerdenbag HJ, Kayser O. Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. J Herb Med 2014;4:51-73.
4. Barus LP. Karo massage oil making ritual in Juma Padang village Barusjahe karo district Buddayah. J Pendidikan Antropologi
5. Sitepu W. Traditional oil from Tanah Karo. kompasiana 2015 February 21st, 2014.
6. Nababan RT. Analisis Kandungan Logam Cu, Fe, Dan Pb Dalam Obat Tradisional Minyak Karo . Medan: North Sumatera University; .
7. Sembiring R, Utomob B, Batubara R. Vegetation diversity of medicinal
plants in the educational forest of the university of North Sumatra, Taman Hutan Raya Tongkoh, Karo Regency, North Sumatra. Peronema For Sci J 2013;2:19-22.
8. Pazyar N, Yaghoobi R, Rafiee E, Mehrabian A, Feily A. Skin wound healing and phytomedicine: A review. Skin Pharmacol Physiol 2014;27:303-10.
9. Pramudya WA. Structural Elucidation of Isolates Resulting from the Chloroform Extract of the Skin of Gowok Plant (Syzygius polycephalum) and Antioxidant Activity Test. Negeri: Mathematics and Science Faculty of Negeri Surabaya University; 2016.
10. Ammar GA. Alkaloid Test. Bandung: Faculty of Math and Science, Bandung Institute of Technology, Department OC; 2014.
11. Kummer R, Estevão-Silva CF, Bastos RL, Rocha BA, Spironello RA, Yamada AN, et al. Alpha-pinene reduces in vitro and in vivo leukocyte migration during acute inflammation. Int J Appl Res Nat Prod 2015;8:12-7.
12. de Cassia da Silveira e Sa R, Andrade LN, de Sousa DP. Areview on anti-inflammatory activity of monoterpenes. Molecules 2013;18:1227-54.
13. Kang E, Lee DH, Jung YJ, Shin SY, Koh D, Lee YH. ?-pinene inhibits tumor invasion through downregulation of nuclear factor (NF)-?B-regulated matrix metalloproteinase-9 gene expression in MDA-MB-231 human breast cancer cells. Appl Biol Chem 2016;59:511-516.
14. Kim DS, Lee HJ, Jeon YD, Han YH, Kee JY, Kim HJ, et al. Alpha-pinene exhibits anti-inflammatory activity through the suppression of MAPKs and the NF-kappaB pathway in mouse peritoneal macrophages. Am J Chin Med 2015;43:731-42.
15. Patil N, Vaishnav RL, Thanusubramanian H, Holla SN, Manohar HD, Bairy KL. Formulation and evaluation of the hydroalcoholic extract of Caesalpinia pulcherrima (Stem bark) on wound healing model in Wistar rats. Int J Adv Res 2015;3:648-54.
16. Krzyszczyk P, Schloss R, Palmer A, Berthiaume F. The role of macrophages in acute and chronic wound healing and interventions to promote pro-wound healing phenotypes. Front Physiol 2018;9:419.
17.Rufino AT, Ribeiro M, Judas F, Salgueiro L, Lopes MC, Cavaleiro C, et al. Anti-inflammatory and chondroprotective activity of (+)-alpha-pinene: Structural and enantiomeric selectivity. J Nat Prod 2014;77:264-9.