IN VITRO ANTIOXIDANT ACTIVITY AND FOURIER TRANSFORM INFRARED ANALYSIS OF ELAEAGNUS CONFERTA ROXB. LEAF EXTRACT
Objective: The objective of this study was to carry out the antioxidant potential of Elaeagnus conferta (EC) Roxb. leaves that were worked on various assay 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization, total reducing power was studied by ferric reducing antioxidant power (FRAP) and total radical trapping antioxidant potential (TRAP) method. The spectroscopic technique Fourier transform infrared (FTIR) spectrophotometer was performed to detect the characteristic peak values and their functional groups of EC Roxb.
Methods: Ethanol solvent is used ABTS, DPPH, FRAP, TRAP, and FTIR analysis.
Results: ABTS assay, IC50 value was found to be 60 μg/ml compared with standard ascorbic acid 64 μg/ml. DPPH IC50 value of EC Roxb. compared with standard ascorbic acid was found to be 0.43 μg/ml and 0.62 μg/ml. FRAP IC50 value along with standard ascorbic acid was found to be 0.582 μg/ml and 0.596 μg/ml. The IC50 value of TRAP along with standard ascorbic acid was found to be 0.39 μg/ml and 0.62 μg/ml. In FTIR spectroscopic study, it revealed different characteristic peak values with various functional compounds such as phenols, alcohols, amide, alkanes, carboxylic acids, alkenes, primary amines, aromatics, ester, alkyl halides, and aliphatic amines compounds.
Conclusion: EC Roxb. leaves have a potent rich source of antioxidant. In FTIR analysis, it is confirmed with the presence of phenolic compounds that can be emphasized for efficacy in pharmaceutics.
2. Qorina F, Arsianti A, Fithrotunnisa Q, Tejaputri NA. Phytochemistry and antioxidant activity of soursop (Annona muricata) leaves. Int JAP 2019;11:1-6.
3. Ragahavendra P, Devi AG, Shyma TB. Investigations of anti-diabetic medicinal plants used by tribes of Wayanad district, Kerala. Int J Pharm sci Res 2015;6:3617-25.
4. Kale A, Gawande S, Kotwal S. Cancer phytotherapeutics: Role for flavonoids at the cellular level. Phytother Res 2008;22:567-77.
5. Gross M, Pfeiffer M, Martini M, Campbell D, Slavin J, Potter J. The quantitation of metabolites of quercetin flavonols in human urine. Cancer Epidemiol Biomarkers Prev 1996;5:711-20.
6. Tepe B, Sokmen M, Akpulat HA, Sokmen A. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem 2006;95:200-4.
7. Anggraini T, Wilma S, Syukri D, Azima F. Total phenolic, anthocyanin, catechins, DPPH radical scavenging activity, and toxicity of Lepisanthes alata (Blume) Leenh. Int J Food Sci 2019;2019:9703176.
8. Mariod AA, Matthaus B, Hussein IH. Antioxidant properties of methanolic extracts from different parts of Sclerocarya birrea. Int J Food Sci Technol 2008;43:921-6.
9. Kumar LG, Karthik, Bhaskara Rao KV. Phytochemical composition and in vitro antimicrobial activity of Bauhinia racemosa Lamk (Caesalpinaceae). Int J Pharm Sci Res 2010;1:51-8.
10. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med 1999;26:1231-7.
11. Elizabeth K, Rao MN. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237-40.
12. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem 1996;239:70-6.
13. Deuschla VC, Cruz RD, Flores VC, Denardi LB, Desuchile, Rossi GG, et al. Persea americana: Phenolic profile, antioxidan potential, antimicrobial activity and in silico prediction of pharmacokinetic and toxicological properties. Indian J Pharm Sci 2019;81:766-75.
14. Giardi MT, Rea G, Berra B. Bio-farms for nutraceuticals. Functional food and safety control by biosensors. Preface. Adv Exp Med Biol 2010;698:7-8.
15. Hari N, Nair V, Iyer H. FTIR spectroscopic analysis of leaf extract in hexane in Jasminum azoricum L. Int J Sci Res Sci Technol 2019;4:1-8.
16. Purena R, Bhatt R. Comparative phytochemical investigation, antioxidant and anticancer properties of leave extracts of four medicinal plants from Chhattisgarh, India. Asian J Plant Sci Res 2018;8:1-21.
17. Sujatha S, Sekar T. Free-radical scavenging activity leaf extract of Litsea laevigata Gamble. Int J Pharm Pharm Sci 2019;11:96-103.
18. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.
19. Ruwali P, Adhikari M, Sharma S. Phytochemical and antioxidant properties of various extracts of Michelia champaca leaves. Int J Pharm Pharm Sci 2019;11:56-61.
20. Halvorsen BL, Carlsen MH, Phillips KM, Bøhn SK, Holte K, Jacobs DR Jr., et al. Content of redox-active compounds (ie, antioxidants) in foods consumed in the United States. Am J Clin Nutr 2006;84:95-135.
21. Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, et al. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. J Nutr 2003;133:2812-9.
22. Abramovic H, Grobin B, Ulrih NP, Cigic B. Relevance and standardization of in vitro antioxidant assays: ABTS, DPPH, and Folin Ciocalteu. Hindawi J Chem 2018;2018:1-9.
23. Sucupira NR, Silva AB, Pereira G, Costa JN. Methods for measuring antioxidant activity of fruits. Cient Cienc BiolSaude 2012;14:263-9.
24. Wang M, Zheng Y, Khuong T, Lovatt CJ. Developmental differences in antioxidant compounds and systems in normal and small-phenotype fruit of “Hass” avocado (Persea americana Mill.). Sci Hortic 2016;206:15-23.
25. Gupta R, Singh RL. Comparative study on the antioxidant activities of methanolic and aqueous extracts of Terminalia bellerica. Int J Curr Pharm Res 2019;11:103-10.
26. Elufioye TO, Mada OO. GC-MS, FTIR, UV analysis and in vitro antioxidant activity of a Nigeria poly herbal mixture: Pax herbal bitters. Free Radic Antioxid 2018;8:74-81.
27. Kumar V, Roy BK. Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis. Sci Rep 2018;8:10714.
28. Duraiswamy B, Singanan M, Varadarajan V. Physicochemical, phytochemicals and antioxidant evaluation of Guazuma ulmifolia fruit. Int J Pharm Pharm Sci 2018;10 Suppl 9:87.
29. Pisoschi AM, Pop A, Cimpeanu C, Predoi G. Antioxidant capacity determination in plants and plant-derived products: A review. Oxid Med Cell Longev 2016;2016:9130976.
30. Collins AS, Sumner SC, Borghoff SJ, Medinsky MA. A physiological model for tert-amyl methyl ether and tert-amyl alcohol: Hypothesis testing of model structures. Toxicol Sci 1999;49:15-28.
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