EVALUATION OF IN VITRO HEPATIC TOXICITY OF LEAVES OF PTEROSPERMUM ACERIFOLIUM (L.) WILLD.

  • RANA DATTA Department of Pharmacology, Gupta College of Technological Sciences, Asansol, West Bengal, India.
  • SANKHADIP BOSE Department of Pharmacognosy, Bengal School of Technology, Hooghly, West Bengal, India.
  • SUDIP KUMAR MANDAL Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Dr. B. C. Roy College of Pharmacy and Allied Health Sciences, Bidhannagar, West Bengal, India.

Abstract

Objective: The objective of the study was to determine the in vitro hepatic toxicity profile of methanolic extract of leaves of Pterospermum acerifolium (L.) Willd. (MEPA) using a mammalian hepatic cell line (HepG2).


Methods: To assess its in vitro hepatic toxicity, 3-(4,5-dimethylthiazol-2-yl)-2,5-2,5-diphenyltetrazolium bromide assay using MEPA at a concentration of 25 μg, 50 μg, 100 μg, 200 μg, and 300 μg was carried out. Sorafenib tosylate was used as the standard agent to assess cytotoxicity.


Results: The inhibitory concentration 50 (IC50) value for HepG2 cell lines was determined after 24 h. Thereafter the cytotoxicity study was again performed with the ½ IC50, IC50, and 2IC50 doses of MEPA. Experimentally, the IC50 was found to be 150.42 μg/ml for HepG2 using MEPA. A dose-dependent cytotoxicity and reduction in optical density were also observed with incremental MEPA administration.


Conclusion: The cytotoxic potential of MEPA was found to be much less than that of sorafenib tosylate. Thus, MEPA shows in vitro cytotoxicity in mammalian hepatic cells (HepG2) at a concentration as low as 100 μg.

Keywords: Pterospermum acerifolium, Cytotoxicity, In vitro, Cell line, Hepatocarcinoma

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DATTA, R., S. BOSE, and S. KUMAR MANDAL. “EVALUATION OF IN VITRO HEPATIC TOXICITY OF LEAVES OF PTEROSPERMUM ACERIFOLIUM (L.) WILLD.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 5, Mar. 2020, pp. 118-20, doi:10.22159/ajpcr.2020.v13i5.36998.
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