• Pavithra Ramasamy Department of Botany, School of Life Sciences, Periyar University, Salem, Tamil Nadu, India.
  • LALITHA SUNDARAM Department of Botany, School of Life Sciences, Periyar University, Salem, Tamil Nadu, India.


Objective: The main objective of this study was to determine the effect of biocontrol Trichoderma species agent against Fusarium oxysporum in Solanum lycopersicum L.

Methods: Genus Trichoderma effective biocontrol agent against fungal, bacterial pathogen. The fungal isolates Trichoderma viride (Tr01), Trichoderma harzianum (Tr02), and F. oxysporum (Fu04) were also identified through morphological characterization observed under a light microscope (10x×40x). The isolates (Tr01, Tr02, Fu04) were confirmed through genetic DNA isolation and polymerase chain reaction analysis. After that pot culture study was conducted to test the antagonistic potential (Tr01, Tr02) and against (Fu04). Seeds of tomato (S. lycopersicum L.) were used in this experiment; treatment such as control, Tr01, Tr02, Fu04, Tr01+ Fu04, and Tr02+ Fu04 was given to the seeds sown in pots and maintained in appropriate distances.

Results: In pot culture after 45th day after inoculation of bioinoculated plant (Tr02), significantly enhances plant length, fresh weight, dry weight, chlorophyll content, nitrogen content, NPK content, and soil microbial population.

Conclusion: In this experiment, Trichoderma spp. to control plant pathogen and improve yield and quality of crop. Fungal species belonging to the genus Trichoderma act as a biological agent. Bioinoculated plant (Tr02) significantly enhances plant growth ability to with stand under nutrient deficient conditions.

Keywords: Antagonistic effects, Chlorophyll, NPK conten, Trichoderma harzianum, Fusarium oxysporum, Trichoderma asperellum


1. Wolfe BE, Klironomos JN. Breaking new ground: Soil communities and exotic plant invasion. BioScience 2005;55:477-87.
2. Monte E. Understanding Trichoderma: Between biotechnology and microbial ecology. Int Microbiol 2001;4:1-4.
3. Amraoui B, Biard J, Kbal F, El Wahidi M, Kandil M. Activity of haliscosamine against Fusarium oxysporum F. sp. Melonis: In vitro and in vivo analysis. Springerplus 2015;4:16.
4. Dighton J, White JF, Oudemans P. The Fungal Community; Its Organization and Role the Ecosystem. 3rd ed. United Kingdom: Taylor & Francis; 2005. p. 93-115.
5. Abdullah A, Ahmad S, Kamaluddeen R. Efficacy of Trichoderma spp, neem products and carbendazim against Fusarium wilt of tomato in pot condition. Int J Agric Sci Res 2013;3:73-80.
6. Dwivedi SK. Effectiveness of some antagonistic fungi and botanicals against Fusarium solani and Fusarium oxysporum infecting brinjal and tomato plants. Asian J Plant 2014;8:18-25.
7. Mallick A, Mitra A. Salicylic acid-induced resistance to Fusarium oxysporum in tomato. Plant Physiol Biochem 2009;8:18-25.
8. Ben-David A, Davidson E. Estimation method for serial dilution experiments. J Microbiol Methods 2014;107:214-21.
9. Oiveira AG, Chagas AF Jr., Santos GR, Miller LO, Chagas LF. Potential phosphate solubIlization and AIA production of Trichoderma spp. Green J Agroecol Sust Dev 2012;7:149-55.
10. Skidmore AM, Dickinson CM. Colony internations and hyphal interference between Saprophytic and phyllophance fungi. Transplant Br Mycol Soc 1976;66:57-64.
11. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning; A Laboratory Manual. United States: Cold Spring Harbor Laboratory Press; 1989.
12. Ressing JL, Strominger JL, Leloir LF. A modified colorimetric method for estimation of N-acetylamine sugars. J Biol Chem 1955;217:959-66.
13. Arnon OI. Copper enzymes in isolated chloroplasts. 1. Polyphenol oxidase in Beta vulgaris. Plant 1949;24:1-15.
14. Subbiah BV, Asija GL. A rapid procedure for determination of available nitrogen in soil. Curr Sci 1956;25:259-60.
15. Olsen SR, Cole CV, Watanabe FS, Dean LA. Estimation of available phosphorus. In: Soil Extraction With Sodium Bicarbonate. US: U. S. Department of Agriculture; 1954. p. 939.
16. Jackson ML. Soil Chemical Analysis. New Delhi: Prentice Hall of India Pvt. Ltd.; 1958. p. 498.
12 Views | 22 Downloads
How to Cite
Ramasamy, P., and L. SUNDARAM. “BIOCONTROL POTENTIAL OF TRICHODERMA SPP. AGAINST FUSARIUM OXYSPORUM IN SOLANUM LYCOPERSICUM L.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 5, Mar. 2020, pp. 156-61, doi:10.22159/ajpcr.2020.v13i5.37108.
Original Article(s)