ACHATINA FULICA MUCUS ATTENUATES ULTRAVIOLET B-INDUCED FIBROBLAST PHOTOAGING THROUGH REDUCING INFLAMMATION, ANGIOGENESIS, AND MATRIX METALLOPROTEINASE

  • CH. TRI NURYANA Doctoral Program of Medicine and Health, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
  • SOFIA MUBARIKA Department of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
  • YOHANES WIDODO WIROHADIDJOJO Department of Dermatology and Venereology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
  • NUR ARFIAN Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
  • PUTU MEGA ADITYA DEVI AYU MARA Undergraduate Program of Medicine and Health, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
  • AHMAD FAIZ HUWAIDI Undergraduate Program of Medicine and Health, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Abstract

Objective: This study aimed to observe the effects of Achatina fulica mucus (AFM) on ultraviolet B (UVB)-induced fibroblast photoaging by assessing monocyte chemotactic protein (MCP)-1, vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-3, and MMP-12 mRNA expressions.


Methods: Cell cultures of normal human dermal fibroblasts (NHDFs) were divided into six groups: Group 1 was normal fibroblasts without UVB irradiation as normal control and Groups 2–5 consisted of 100 mJ/cm2 UVB-induced aged fibroblasts. Group 2 had no treatment as negative control, Group 3 was treated by platelet-rich plasma 10% as positive control group, and Groups 4–6 were treated by various concentrations of AFM (3.9 μl, 15.625 μl, and 62.5 μl). The MCP-1, VEGF, MMP-3, and MMP-12 mRNA expressions in the different NHDF groups were assessed by quantitative polymerase chain reaction.


Results: The mRNA expressions of MCP-1, VEGF, MMP-3, and MMP-12 in the AFM group compared to the UVB group decreased 8, 5, 5, and 4 folds, respectively. AF62 exhibited the highest improvement among the other AFM-treated groups.


Conclusion: AFM treatment attenuates UVB-induced fibroblasts photoaging by reducing inflammation, angiogenesis, and matrix metalloproteinases.

Keywords: Ultraviolet B, Photoaging, Achatina fulica, Matrix metalloproteinase, Inflammation, Angiogenesis

References

1. Amaro-Ortiz A, Yan B, D’Orazio J. Ultraviolet radiation, aging and the skin: Prevention of damage by topical cAMP manipulation. Molecules 2014;19:620-19.
2. Gilchrest BA. Photoaging. J Invest Dermatol 2013;133:E2-6.
3. Scharffetter-Kochanek K, Brenneisen P, Wenk J, Herrmann G, Ma W, Kuhr L, et al. Photoaging of the skin from phenotype to mechanisms. Exp Gerontol 2000;35:307-16.
4. Cho S, Shin MH, Kim YK, Seo JE, Lee YM, Park CH, et al. Effects of infrared radiation and heat on human skin aging in vivo. J Investig Dermatol Symp Proc 2009;14:15-9.
5. Cavinato M, Jansen-Dürr P. Molecular mechanisms of UVB-induced senescence of dermal fibroblasts and its relevance for photoaging of the human skin. Exp Gerontol 2017;94:78-82.
6. Ramdan RD, Sunendar B, Hermawan H. Naturally derived biomaterials and its processing. In: Mahyudin F, Hermawan H, editors. Biomaterials and Medical Devices. Vol. 58. Cham: Springer International Publishing; 2016. p. 23-39.
7. de la Secreción P. Assessment of antimicrobial activity and healing potential of mucous secretion of Achatina fulica. Int J Morphol 2012;30:365-73.
8. Borkow G. Using copper to improve the well-being of the skin. Curr Chem Biol 2014;8:89-102.
9. Raman K, Arungundram S, Kuberan B. Chemogenesis of an antiangiogenic glycosaminoglycan. ACS Med Chem Lett 2014;5:644-6.
10. Kang JS, Kim HN, Kim JE, Mun GH, Kim YS, Cho D, et al. Regulation of UVB-induced IL-8 and MCP-1 production in skin keratinocytes by increasing Vitamin C uptake via the redistribution of SVCT-1 from the cytosol to the membrane. J Invest Dermatol 2007;127:698-706.
11. Cho JM, Lee YH, Baek RM, Lee SW. Effect of platelet-rich plasma on ultraviolet b-induced skin wrinkles in nude mice. J Plast Reconstr Aesthet Surg 2011;64:e31-9.
12. Richarz NA, Boada A, Carrascosa JM. Angiogenesis in dermatology-insights of molecular mechanisms and latest developments. Actas Dermosifiliogr 2017;108:515-23.
13. Hirakawa S, Fujii S, Kajiya K, Yano K, Detmar M. Vascular endothelial growth factor promotes sensitivity to ultraviolet B-induced cutaneous photodamage. Blood 2005;105:2392-9.
14. Yano K, Oura H, Detmar M. Targeted overexpression of the angiogenesis inhibitor thrombospondin-1 in the epidermis of transgenic mice prevents ultraviolet-b-induced angiogenesis and cutaneous photo-damage. J Invest Dermatol 2002;118:800-5.
15. Ghosh AK, Hirasawa N, Lee YS, Kim YS, Shin KH, Ryu N, et al Inhibition by acharan sulphate of angiogenesis in experimental inflammation models. Br J Pharmacol 2002;137:441-8.
16. Jab?o?ska-Trypu? A, Matejczyk M, Rosochacki S. Matrix metalloproteinases (MMPs), the main extracellular matrix (ECM) enzymes in collagen degradation, as a target for anticancer drugs. J Enzyme Inhib Med Chem 2016;31:177-83.
17. Freitas-Rodríguez S, Folgueras AR, López-Otín C. The role of matrix metalloproteinases in aging: Tissue remodeling and beyond. Biochim Biophys Acta Mol Cell Res 2017;1864:2015-25.
18. Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci 2016;17:868.
Statistics
15 Views | 26 Downloads
Citatons
How to Cite
NURYANA, C. T., S. MUBARIKA, Y. W. WIROHADIDJOJO, N. ARFIAN, P. M. A. DEVI AYU MARA, and A. FAIZ HUWAIDI. “ACHATINA FULICA MUCUS ATTENUATES ULTRAVIOLET B-INDUCED FIBROBLAST PHOTOAGING THROUGH REDUCING INFLAMMATION, ANGIOGENESIS, AND MATRIX METALLOPROTEINASE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 5, Apr. 2020, pp. 199-03, doi:10.22159/ajpcr.2020.v13i5.37284.
Section
Original Article(s)