• MADHUMITA GHOSH DASTIDAR Department of Microbiology, Vijaya College, Bengaluru, Karnataka, India.
  • NIVEDITHA BS Department of Microbiology, Vijaya College, Bengaluru, Karnataka, India.
  • POOJA R Department of Microbiology, Vijaya College, Bengaluru, Karnataka, India.




Biofilm, Iron Nanoparticles, Scanning electron microscopy, Fourier transform infrared, Energy-dispersive X-ray analysis


Objective: The objective of this study was to observe the effects of iron nanoparticles (FeNPs) synthesized from plant source of biofilm-forming bacteria.

Methods: FeNPs were synthesized from Pongamia pinnata leaf extracts and it was characterized using ultraviolet–visible spectrophotometer, scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, and energy-dispersive X-ray analysis (EDAX). The synthesized FeNPs were evaluated against biofilm-forming Gram-negative Pseudomonas, sewage organisms, and Gram-positive hay Bacillus, Bacillus subtilis. These biofilm-forming microorganisms were evaluated for antibiotic sensitivity. The extracellular and intracellular proteins of biofilm-forming bacteria were estimated in the presence of FeNPs.

Results: All these biofilm-forming microorganisms were found to be antibiotic resistant. The green FeNPs showed potential antimicrobial effectiveness against hay Bacillus followed by Pseudomonas and sewage bacteria. These nanoparticles inhibited the intracellular protein formation more than extracellular proteins of biofilm-forming microorganisms.

Conclusions: It can be concluded that the FeNPs synthesized from plant sources were effectively inhibited the biofilm-forming microorganisms by obstructing the intracellular protein synthesis. These nanoparticles can be used as an eco-friendly, cost-effective, and alternative molecule to treat the antibiotic-resistant biofilm-forming microorganisms.


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How to Cite

GHOSH DASTIDAR, M., N. BS, and P. R. “EFFECTS OF GREEN IRON NANOPARTICLES ON BIOFILM-FORMING BACTERIA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 6, June 2020, pp. 93-97, doi:10.22159/ajpcr.2020.v13i6.37304.



Original Article(s)