• CHANCHAL GARG Department of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, Haryana, India.
  • RAVINDER SINGH Department of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, Haryana, India.
  • MUNISH GARG Department of Pharmaceutical Sciences, Maharshi Dayanand University Rohtak, Haryana, India.



Anti-aging, Anti-wrinkle, Cucumis sativus, Antioxidant, Hyaluronidase, Elastase, Matrix metalloproteinase-1, Tyrosinase


Objective: The present study was designed to screen the anti-aging and anti-wrinkle potential of Cucumis sativus fruit through in vitro estimation of antioxidant, anti-hyaluronidase, anti-elastase, anti-collagenase/anti-matrix metalloproteinase (MMP)-1, and anti-tyrosinase activity.

Methods: Raw juice of cucumber was taken, filtered and fractionated with ethyl acetate and n-butanol. The obtained extracts were then evaluated for their antioxidant potential through 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay taking ascorbic acid as positive control and other enzymatic activities in reference to hyaluronidase inhibition, MMP-1/collagenase inhibition, and elastase inhibition taking catechin as reference standard whereas for tyrosinase inhibition the standard used was quercetin.

Results: All the evaluations were performed in triplicates and results were noted down. It was observed that aqueous extract of C. sativus fruits showed a maximum DPPH radical scavenging activity (p<0.0001), half-maximal inhibitory concentration (IC50) at a concentration of 122.67 μg/ml. The ethyl acetate fraction of C. sativus fruits exhibited maximum hyaluronidase (p<0.0001), MMP-1/collagenase (p<0.04), and tyrosinase (p<0.04) inhibitory activity, IC50 at a concentration of 59.54, 45.79, and 24.46 μg/ml, respectively. The elastase (p<0.0001) inhibitory activity by n-butanol fraction of C. sativus fruits extract was maximum, IC50 at a concentration of 52.76 μg/ml.

Conclusion: A potent anti-aging and anti-wrinkle properties were well demonstrated by C. sativus, as depicted from the results obtained.


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Markrantonaki E, Zouboulis CC. Molecular mechanisms of skin aging: State of the art. Ann N Y Acad Sci 2007;1119:40-50.

Nema NK, Maity M, Sarkar BK, Mukherjee PK. Matrix metalloproteinase, hyaluronidase and elastase inhibitory potential of standardized extract of Centella asiatica. Pharm Biol 2013;51:1182-7.

Widowati W, Fauziah N, Herdiman H, Afni M, Afifah E, Sari H, et al. Antioxidant and anti-aging assays of Oryza sativa extracts, vanillin and coumaric acid. J Nat Remedies 2016;16:2320-58.

Ndlovu G, Fouche G, Tselanyane M, Cordier W, Steenkamp V. In vitro determination of the anti-aging potential of four Southern African medicinal plants. BMC Complement Alter Med 2013;13:304.

Ganceviciene R, Liakou AI, Theodoridis A, Makrantonaki E, Zouboulis CC. Skin anti-aging strategies. Dermato Endocrinol 2012;4:308-19.

Manuskiatti W, Maibach HI. Hyaluronic acid and skin: Wound healing and aging. Int J Dermatol 1996;35: 2397-404.

Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: A key molecule in skin aging. Dermato Endocrinol 2012;4:253-8.

Briganti S, Camera E, Picardo M. Chemical and instrumental approaches to treat hyperpigmentation. Pigm Cell Res 2003;16:101-10.

Sturm RA, Teasdale RD, Box NF. Human pigmentation genes: Identification, structure and consequences of polymorphic variation. Gene 2001;277:49-62.

Sahu T, Sahu J. Cucumis sativus (cucumber): A review on its pharmacological activity. J Appl Pharm Res 2015;3:4-9.

Patri G, Silano V, Anton R. Plants in Cosmetics. Strasbourg: Council of Europe Publishing; 2002.

Warrier PK. Indian Medicinal Plants: A Compendium of 500 Species. Chennai: Orient Longman; 1994.

Shah P, Dhande S, Joshi Y, Kadam V. A review on Cucumis sativus (cucumber). Res J Pharm Phytochem 2013;5:49-53.

Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Nat Prod 2001;64:892-5.

Lee BC, Lee SY, Lee HJ, Sim GS, Kim JH, Kim J. Anti-oxidative and photo protective effects of coumarins isolated from Fraxinus chinensis. Arch Dermatol Res 2007;30:1293-301.

Kim YJ, Uyama H, Kobayashi S. Inhibition effects of (+)-catechin-aldehyde polycondensates on proteinases causing proteolytic degradation of extracellular matrix. Biochem Biophys Res Commun 2004;320:256-61.

Pientaweeratch S, Panapisa V, Tansirikongkol Y. Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica, Manilkara zapota and silymarin: An in vitro comparative study for anti-aging applications. Pharm Biol 2016;54:1865-72.

Hsu C, Chang C, Lu H, Chung Y. Inhibitory effects of the water extracts of Lavandula sp. on mushroom tyrosinase activity. Food Chem 2007;105:1099-105.

Wang BS, Chang LW, Wu HC, Huang SL, Chu HL, Huang MH. Antioxidant and antityrosinase activity of aqueous extracts of green asparagus. Food Chem 2011;127:141-6.

Jiménez M, Chazarra S, Escribano J, Cabanes J, García-Carmona F. Competitive inhibition of mushroom tyrosinase by 4-substituted benzaldehydes. J Agric Food Chem 2001;49:4060-3.

Kubo I, Kinst-Hori I. Tyrosinase inhibitory activity of the olive oil flavor compound. J Agric Food Chem 1999;47:4574-8.

Sumathi T, Ponnuswami V, Senthamizh BS. Anatomical changes of cucumber (Cucumis sativus L.) leaves and roots as influenced by shade vand fertigation. Res J Agric Biol Sci 2008;4:630-8.

Mallik J, Akhter R. Phytochemical screening and in vitro evaluation of reducing power, cytotoxicity and anti-fungal activities of ethanol extracts of Cucumis sativus. Int J Pharm Biol Sci Arch 2012;3:555-60.

Tiwari, P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Int Pharm Sci 2011;1:98-106.

Belitz HD, Grosch W. Química de los Alimentos. Zaragoza: Editorial Acribia S.A; 1997. p. 825-60.

Musmade AM, Desai UT. Cucumber and melon. In: Handbook of Vegetable Science and Technology: Production, Composition, Storage, and Processing. New York: Marcel Dekker, Inc.; 1998. p. 245-72.



How to Cite

GARG, C., R. SINGH, and M. GARG. “IN VITRO SCREENING OF ANTIOXIDANT AND ANTIAGING POTENTIAL OF CUCUMIS SATIVUS FRUIT EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 5, May 2020, pp. 187-90, doi:10.22159/ajpcr.2020.v13i5.37258.



Original Article(s)