BRINE SHRIMPS LETHALITY TEST OF ETHANOL EXTRACT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE FRACTION OF BLIGHIA SAPIDA

  • ADEKOLA MB Department of Environmental Management and Toxicology, Federal University of Agriculture, Abeokuta.
  • AREOLA JO Department of Medical Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • ORIYOMI OV Institute of Ecology and Environmental Studies, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • APATA JT Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • APALOWO OE Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • ADESINA AF Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • BABALOLA OO Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.

Abstract

Objective: The objective of the study was to evaluate brine shrimp lethality of Blighia sapida stem-bark extract and its fractions and identify the bioactive constituents in the ethyl acetate fraction (EAF) using gas chromatography-mass spectrometry (GC-MS) technique.


Methods: The ethanol extract (EE) and its fractions were subjected to lethality assay, and GC-MS analysis of EAF was carried out.


Results: The lethality test showed a concentration-dependent mortality rate in the brine shrimp nauplii for the EE and its fractions. GC-MS analysis of EAF of the extract revealed the existence of 13 peaks of the GC-MS chromatogram with only one prominent compound, n-hexadecanoic acid (peak area of 10.13%).


Conclusion: The result revealed the presence of 13 bioactive components in the EAF of the extract, the majority of which have been reported for different biological activities, hence, justifies the use of the plant in the treatment and management of different diseases ethnomedicinally.

Keywords: Brine shrimp, Lethality, Blighia sapida, Crude extract, Ethyl acetate, Gas chromatography-mass spectrometry

Author Biographies

AREOLA JO, Department of Medical Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.

Department of Medical Biochemistry.

ORIYOMI OV, Institute of Ecology and Environmental Studies, Obafemi Awolowo University, Ile-Ife, Nigeria.

Student

APATA JT, Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.

student

APALOWO OE, Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.

Student

ADESINA AF, Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.

Student

BABALOLA OO, Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.

Lecturer

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MB, A., A. JO, O. OV, A. JT, A. OE, A. AF, and B. OO. “BRINE SHRIMPS LETHALITY TEST OF ETHANOL EXTRACT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE FRACTION OF BLIGHIA SAPIDA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 8, Aug. 2020, pp. 208-12, doi:10.22159/ajpcr.2020.v13i8.37613.
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