IN VITRO ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF PYRENACANTHA VOLUBILIS (EEPV)

  • ANJALI P Department of Pharmacology, College of Pharmacy, Mother Theresa Post Graduate and Research Institute of Health Sciences, Pondicherry, India.
  • VIMALAVATHINI R Department of Pharmacology, College of Pharmacy, Mother Theresa Post Graduate and Research Institute of Health Sciences, Pondicherry, India.
  • KAVIMANI S Department of Pharmacology, College of Pharmacy, Mother Theresa Post Graduate and Research Institute of Health Sciences, Pondicherry, India.

Abstract

Objectives: The study was undertaken to evaluate the in vitro anti-inflammatory and anti-arthritic activity of the ethanolic extract of leaves of Pyrenacantha volubilis (EEPV) using human red blood cells (HRBCs) membrane stabilization and protein denaturation methods.


Methods: In the present study, the in vitro anti-inflammatory and anti-arthritic activity of EEPV was carried out using HRBC membrane stabilization by hypotonicity-induced hemolysis and protein denaturation using egg albumin methods at various concentrations (100, 200, 400, 800, and 1000) of EEPV. Diclofenac sodium was used as reference standard.


Results: P. volubilis was effective in inhibiting HRBC membrane stabilization and protein denaturation in a dose-dependent manner and was comparable to the standard drug diclofenac sodium.


Conclusion: The study suggests that P. volubilis has potential anti-inflammatory and anti-arthritic activity.

Keywords: Pyrenacantha volubilis, Anti-inflammatory, Anti-arthritic activity

References

1. Anjali P, Vimalavathini R. Role of metalloproteinases in the pathophysiology of rheumatoid arthritis. WJPR 2020;9:1266-70.
2. Suma HK, Kumar V, Senthilkumar U, Kumara PM, Ravikanth G, Santhoshkumar TR, et al. Pyrenacantha volubilis wight, (Icacinaceae) a rich source of camptothecine and its derivatives, from the Coromandel Coast forests of India. Fitoterapia 2014;97:105-10.
3. Anjali P, Vimalavathini R, Kavimani S. In silico docking of camptothec in with the pathological mediators of rheumatoid arthritis. EAS J Pharm Pharmacol 2020;2:18-22.
4. Jackson JK, Higo T, Hunter WL, Burt HM. Topoisomerase inhibitors as anti-arthritic agents. Inflamm Res 2008;57:126-34.
5. Leelaprakash G, Dass SM. In-vitro anti-inflammatory activity of methanolic extract of Enicostemma axillare. IJDDR 2011;3:189-96.
6. Vaijayanthimala P, Sakthipriya M, Sangameswaran B. In-vitro anti-arthritic activity of Cissus quandrangularis stem extract. Asian J Pharm Clin Res 2019;12:250-2.
7. Chandra K, Salman AS, Mohd A, Sweety R, Ali KN. Protection against FCA induced oxidative stress induced DNA damage as a model of arthritis and in-vitro anti-arthritic potential of Costus speciosus rhizome extract. Int J Pharmacogn Phytochem Res 2015;7:383-9.
8. Kumari CS, Yasmin N, Hussain MR, Babuselvam M. In vitro anti-inflammatory and anti-arthritic property of Rhizophora mucronata leaves. Int J Pharma Sci Res 2015;6:482-5.
9. Desphande V, Jadhav VM, Kadam VJ. In-vitro anti-arthritic activity of Abutilon indicum (Linn) sweet. IJPP 2009;2:644-5.
10. Alamgeer, Niazi SG, Uttra A, Qaiser MN, Ahsan H. Appraisal of anti-arthritic and nephroprotective potential of Cuscuta reflexa. Pharm Biol 2017;55:792-8.
11. Karthik EV, Vishnupriya V, Gayathri R. Anti-inflammatory activity of lavender oil using human RBC membrane stabilizing method. Int J Pharm Sci Rev Res 2016;40:254-8.
12. Fawole OA, Ndhlala AR, Amoo SO, Finnie JF, Van Staden JV. Anti-inflammatory and phytochemical properties of twelve medicinal plants used for treating gastro-intestinal ailments in South Africa. Ethnopharmacology 2009;123:237-43.
13. Kang KH, Kong CS, Seo Y, Kim MM, Kim SK. Anti-inflammatory effect of coumarins isolated from Corydalis heterocarpa in HT-29 human colon carcinoma cells. Food Chem Toxicol 2009;47:2129-34.
Statistics
28 Views | 40 Downloads
Citatons
How to Cite
P, A., V. R, and K. S. “IN VITRO ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF PYRENACANTHA VOLUBILIS (EEPV)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 8, June 2020, pp. 156-8, doi:10.22159/ajpcr.2020.v13i8.38017.
Section
Original Article(s)