EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY OF CITRUS MACROPTERA MONTR

  • SONAM BHUTIA Department of Pharmacognosy, Government Pharmacy College, Government of Sikkim, Sikkim University, Rumtek, Sajong, East Sikkim.

Abstract

Objective: The use of naturally occurring medicines dependent on essential oils (EOs) is nowadays of great interest. In addition, within the human body, EO shows high efficacy as antioxidants and anti-inflammatory drugs. The present experiment was conducted to access the anti-inflammatory activity of EO obtained from the fruit peels of Citrus macroptera Montr. (Rutaceae) against the denaturation of protein in vitro model.


Methods: The test sample (EO) was incubated under controlled laboratory conditions at varying concentrations with egg albumin and was subjected to absorbance determination for the anti-inflammatory property analysis. Diclofenac sodium was used as the standard reference drug for the experiment.


Results: The results show a concentration-dependent inhibition of protein (albumin) denaturation by the test oil. This was concluded by comparing their IC50 average values. Citrus macroptera Montr. EO possessed IC50 average value 54.6+0.07 μg/mL, whereas that of diclofenac sodium was found to be 52.89+0.06 μg/ml. The result shows that the test oil is more effective than the standard drug.


Conclusion: From the above experimental finding, it can be concluded that Citrus macroptera Montr. EO has significance anti-inflammatory effect against the denaturation of the protein in vitro model. The activity may be due to the presence of terpene polyphenolic component or some other active compound present in the oil. The provided information was first of its kind of knowledge to keep the scientific data for future reference.

Keywords: Anti-inflammatory, essential oil, Citrus macroptera, Protein denaturation, Polyphenolic compounds

References

1. Leelaprakash G, Dass SM. In vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2011;3:189-96.
2. Lalrinzuali K, Vabeiryureilai M, Jagetia GC. Investigation of the anti-inflammatory and analgesic activities of ethanol extract of stem bark of sonapatha Oroxylum indicum in vivo. Int J Inflamm 2016.
3. De Almeida Barros TA, De Freitas LA, Filho JM, Nunes XP, Giulietti AM, De Souza GE, et al. Antinociceptive and anti-inflammatory properties of 7-hydroxycoumarin in experimental animal models: Potential therapeutic for the control of inflammatory chronic pain. J Pharm Pharmacol 2010;62:205-13.
4. Divya TS, Latha PG, Usha K, Anuja GI, Suja SR, Shyamal S, et al. Anti-inflammatory, analgesic and anti-lipid peroxidative properties of Wattakaka volubilis (Linn. F.) Stapf. Natl Prod Rad 2009;8:137-41.
5. Heidelberger M, Kendall FE. A quantitative theory of the precipitin reaction: III. The reaction between crystalline egg albumin and its homologous antibody. J Exp Med 1935;62:697-720.
6. Vinueza D, Lopez E, Acosta K, Abdo S. Assessment of anti-inflammatory activity and cytotoxicity of freeze dried hydroalcoholic extract of Bidens andicola on isolated neutrophils. Asian J Pharma Clin Res 2017;10:160-3.
7. Fraternale D, Flamini G, Ascrizzi R. In vitro anticollagenase and antielastase activities of essential oil of Helichrysum italicum subsp. Italicum (Roth) G. Don. J Med Food 2019;22:1041-6.
8. Williams LA, O’Connar A, Latore L, Dennis O, Ringer S, Whittaker JA, et al. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med J 2008;57:327-31.
9. Biradar S, Kangralkar VA, Mandavkar Y, Thakur M, Chougule N. Antiinflammatory, antiarthritic, analgesic and anticonvulsant activity of Cyperus essential oils. Int J Pharm Pharm Sci 2010;2:112-5.
10. Perrone MR, Artesani MC, Viola M, Gaeta F, Caringi M, Quaratino D, et al. Tolerability of rofecoxib in patients with adverse reactions to nonsteroidal anti-inflammatory drugs: A study of 216 patients and literature review. Int Arch Allergy Immunol 2003;132:82-6.
11. Lourens AC, Reddy D, Ba?er KH, Viljoen AM, Van Vuuren SF. In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species. J Ethnopharmacol 2004;95:253-8.
12. Chou ST, Lai CP, Lin CC, Shih Y. Study of the chemical composition, antioxidant activity and anti-inflammatory activity of essential oil from Vetiveria zizanioides. Food Chem 2012;134:262-8.
13. Siani AC, Ramos MD, Menezes-de-Lima O Jr., Ribeiro-dos-Santos R, Fernadez-Ferreira E, Soares RO, et al. Evaluation of anti-inflammatory-related activity of essential oils from the leaves and resin of species of Protium. J Ethnopharmacol 1999;66:57-69.
14. Nongalleima KH, Ajungla T, Singh CB. GC-MS based metabolic profiling of essential oil of Citrus macroptera montruz leaves and peel, assessment of in vitro antioxidant and anti-inflammatory activity. Int J Pharm Pharm Sci 2017;9:107-4.
15. Manjamalai A, Valavil S, Grace V. Evaluation of essential oil of Tridax procumbens L. For anti-microbial and antiinflammatory activity. Int J Pharm Pharm Sci 2012;4:356-63.
16. Al-Reza SM, Yoon JI, Kim HJ, Kim JS, Kang SC. Anti-inflammatory activity of seed essential oil from Ziziphus jujuba. Food Chem Toxicol 2010;48:639-43.
17. Baricevic D, Sosa S, Loggia RD, Tubaro A, Simonovska B, Krasna A, et al. Topical anti-inflammatory activity of Salvia officinalis L. leaves: The relevance of ursolic acid. J Ethnopharmacol 2001;75:125-32.
Statistics
40 Views | 46 Downloads
Citatons
How to Cite
BHUTIA, S. “EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY OF CITRUS MACROPTERA MONTR”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 8, May 2020, pp. 101-3, doi:10.22159/ajpcr.2020.v13i8.38063.
Section
Original Article(s)