• ASWATHI P Department of Botany, University of Calicut, Cell and Molecular Biology Division, Malappuram, Kerala, India.
  • JOHN ERNEST THOPPIL Department of Botany, University of Calicut, Cell and Molecular Biology Division, Malappuram, Kerala, India.


Objective: The present study is mainly focused on the antigenotoxicity and antioxidant potential of the fruit extract of an important dye yielding plant Terminalia paniculata Roth.

Methods: Genoprotective activity of the fruit extract was studied by Allium cepa root tip assay. Three modes of treatment were used to perform the antigenotoxicity, that is, pre-treatment, post-treatment, and simultaneous treatment. For the study of antioxidant potential, four different assays were performed.

Results: Antigenotoxicity studies revealed the protective role of the extract in chromosomal aberrations induced by 2% hydrogen peroxide (H2O2) on A. cepa root tip meristem. The fruit extract showed a significant modulatory effect by means of an inhibition percentage and also it showed a characteristic reversing of chromosomal aberrations induced by H2O2. While in the case of antioxidant activity, the plant extract showed an appreciable antioxidant potential. Four different assays were used to determine the antioxidant potential of T. paniculata. Of these 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging activity revealed almost equal effect to that of the standard.

Conclusions: In the present investigation, it has been found that the important dye yielding plant T. paniculata has a significant role in various commercial industries such as food, cosmetics, clothes, and pharmaceutics due to its efficient protective role.

Keywords: Genotoxicity, Oxidative stress, Terminalia paniculata, Antioxidant potential


1. Prajitha V, Thoppil JE. Induction of giant cells by the synthetic food colorants viz. lemon yellow and orange red. Cytotechnology 2016;68:443-50.
2. Vazhangat P, Thoppil JE. Apoptotic induction via membrane/DNA damage and metabolic inactivation by synthetic food colorants in Allium cepa root meristem. Turk J Biol 2016;40:922-33.
3. Halliwell B, Gutteridge JM. The definition and measurement of antioxidants in biological systems. Free Radic Biol Med 1995;18:125-6.
4. Li Y, Trush MA. Reactive oxygen-dependent DNA damage resulting from the oxidation of phenolic compounds by a copper-redox cycle mechanism. Cancer Res 1994;54:1895-8.
5. Nose K. Role of reactive oxygen species in the regulation of physiological functions. Biol Pharm Bull 2000;23:897-903.
6. Valko M, Rhodes C, Moncol J, Izakovic MM, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol 2006;160:1-40.
7. Celik K, To?ar B, Türkez H, Ta?pinar N. In vitro cytotoxic, genotoxic, and oxidative effects of acyclic sesquiterpene farnesene. Turk J Biol 2014;38:253-9.
8. Demir E, Kaya N, Kaya B. Genotoxic effects of zinc oxide and titanium dioxide nanoparticles on root meristem cells of Allium cepa by comet assay. Turk J Biol 2014;38:31-9.
9. Sharma S, Vig AP. Antigenotoxic effects of Indian mustard Brassica juncea (L.) Czern aqueous seeds extract against mercury (Hg) induced genotoxicity. Sci Res Essays 2012;7:1385-92.
10. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem 2001;49:3420-4.
11. Kunchandy E, Rao MN. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237-40.
12. Kumaran A. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem 2006;97:109-14.
13. Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. Antioxidative properties of cardoon (Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. J Agric Food Chem 2002;50:4989-93.
14. Fitzpatrick FA. Inflammation, carcinogenesis and cancer. Int Immunopharmacol 2001;1:1651-67.
15. Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 2006;141:391-6.
16. Maurich T, Pistelli L, Turchi G. Anti-clastogenic activity of two structurally related pterocarpans purified from Bituminaria bituminosa in cultured human lymphocytes. Mutat Res 2004;561:75-81.
17. Rodrigues CR, Dias JH, de Mello RN, Richter MF, Picada JN, Ferraz AB. Genotoxic and antigenotoxic properties of Baccharis trimera in mice. J Ethnopharmacol 2009;125:97-101.
18. Brown EJ, Khodr H, Hider CR, Rice-Evans CA. Structural dependence of flavonoid interactions with Cu2+ ions: Implications for their antioxidant properties. Biochem J 1998;330:1173-8.
19. Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 2001;73:239-44.
20. Chatha SA, Hussain AI, Asad R, Majeed M, Aslam N. Bioactive components and antioxidant properties of Terminalia arjuna L. extracts. Int J Food Proc Technol 2014;5:1.
21. Walia H, Kumar S, Arora S. Comparative antioxidant analysis of hexane extracts of Terminalia chebula Retz. Prepared by maceration and sequential extraction method. J Med Plant Res 2011;5:2608-16.
22. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49:5165-70.
23. Cai Y, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem 2003;51:2288-94.
24. Devi SE, Thoppil JE. Cytotoxic studies and phytochemical analysis of Orthosiphon thymiflorus (Roth) Sleesen. Int J Pharm 2016;8:249-55.
25. Ravindran AE, Thoppil JE. Enigmatic induction of cytomixis in Allium cepa root meristem by Aglaia edulis Roxb. leaf extract and its phytochemical rationale. Asian J Pharm Clin Res 2020;13:168-71.
26. Rubeena M, Thoppil JE. Cytotoxic potential of Cissus quandrangularis on Allium cepa root meristem. Int J Adv Pharm Sci 2018;10:1-6.
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How to Cite
P, A., and J. ERNEST THOPPIL. “ASSESSMENT OF ANTIOXIDANT POTENTIAL AND ANTIGENOTOXICITY ANALYSIS THROUGH HYDROGEN PEROXIDE-INDUCED OXIDATIVE STRESS IN TERMINALIA PANICULATA ROTH”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 8, May 2020, pp. 108-12, doi:10.22159/ajpcr.2020.v13i8.38123.
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