THE PHYTOCHEMICAL SCREENING HEXANE AND METHANOL EXTRACT OF SINYO NAKAL (DURANTA REPENS)

Authors

  • PURWANTININGSIH SUGITA Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia.
  • RIZKI AMILIA Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia.
  • BUDI ARIFIN Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia.
  • DYAH UTAMI CAHYANING RAHAYU Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia.
  • HANHAN DIANHAR Chemistry Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i8.38165

Keywords:

Duranta repens, Unsaponifiable hexane fraction, Methanol extract

Abstract

Objective: Phytochemicals are naturally present in every part of plants range from leaves, stem bark, and fruits to roots. The use of plant-based medicine is popular among individuals and communities in developing countries. This study evaluated that the phytochemical profile of Duranta repens fruits was collected from Jombang, East Java, Indonesia.

Methods: Air-dried D. repens fruits were macerated by n-hexane and methanol, a separate flask. n-Hexane extract, then underwent saponification to remove fat. Then, the unsaponifiable of n-hexane and methanol extract was tested through phytochemical screening, respectively.

Results: The methanol extract showed the presence of alkaloids, flavonoids, tannins, and steroids, but the unsaponifiable fraction only contained steroids. The unsaponifiable fraction was found to be a mixture of hydrocarbons ranging from C13-C20, fatty acids (palmitic and linoleic acid), squalene, and Vitamin E.

Conclusion: The phytochemical screening of the plant plays an important role for pharmaceutical studies especially discovering new potential drugs for the treatment of various diseases.

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References

Puri AV. Duranta repens linn. (Verbenaceae): A comprehensive review of pharmacognostic, ethnomedicinal, pharmacological, and phytochemical aspects. Asian J Pharm Clin Res 2018;11:91-6.

Wiart C. Medicinal Plants of Asia and The Pacific. Boca Raton, US: Taylor and Francis; 2011.

Andreu MG, Friedman MH, McKenzie M, Quintana HV, Northrop RJ. Duranta erecta, Golden Dewdrop. Florida, US: University of Florida IFAS Extension; 2010.

Jayalakshmi B, Raveesha KA, Amruthesh KN. Phytochemical investigations and antibacterial activity of some medicinal plants againts pathogenic bacteria. J Appl Pharm Sci 2011;1:124-8.

Nikkon F, Habib MR, Karim MR, Hossain MS, Mosaddik MA, Haque ME. Antishigellosis and cytotoxic potency of crude extracts and isolated constituents from Duranta repens. Mycobiology 2008a;36:173-7.

Sharma P, Khandelwal S, Singh T, Vijayvergia R. Phytochemical analysis and antifungal potential of Duranta erecta against some phytopathogenic fungi. Int J Pharm Sci Res 2012;3:2686-9.

Hiradate S, Yada H, Ishii T, Nakajima N, Ohnishi-Kameyama M, Sugie H, et al. Three plant growth inhibiting saponins from Duranta repens. Phytochemistry 1999;52:1223-8.

Nikkon F, Hasan S, Rahman MH, Hoque MA, Mosaddik MA, Haque ME. Biochemical, hematological and histiphatological effects of Duranta repens stem on rats. Asian J Biochem 2008b;2:366-72.

Nikkon F, Saud ZA, Hossain K, Parvin MS, Haque ME. Larvicidal effects of stem and fruits of Duranta repens against the mosquito Culex quinquefasciatus. J Pharm Tech Res 2009;4:1709-13.

Serena MM, Balasubraman M, Rajan K, Gerald IA. Evaluation of the larvicidal activity of the leaf extracts of Duranta erecta Linn. (Verbenaceae) on the larvae of Culex quinquefascitatus (Say) (Culicidae). J Biopestic 2010;3:582-5.

Adu F, Gbedema SY, Brown P, Annan K, Boamah VE. Antibacterial and free radical scavenging activity of Duranta plumieri Linn. J Pharm Sci Res 2011;2:282-7.

Shahat AA, Nazif NM, Abou-Setta LM, Ibrahim NA, Cos P, Miert SV, et al. Phytochemical investigation and antioxidant activity of Duranta repens. Phytother Res 2005;19:1071-3.

Abou-Setta LM, Nazif NM, Shahat AA. Phytochemical investigation and antiviral activity of Duranta repens. J Appl Sci Res 2007;3:1426-33.

Ahmad N, Zeb F, Ahmad I, Wang F. Repenins A-D, four new antioxidative coumarinolignoids from Duranta repens Linn. Bioorg Med Chem Lett 2009;19:3521-4.

Anis I, Anis E, Ahmed S, Mustafa G, Malik A, Amtul Z, et al. Thrombin inhibitory constituents from Duranta repens. Helvchim Acta 2001;84:649-55.

Iqbal K, Malik A, Mukhtar N, Anis I, Khan SN, Iqbal M. Alpha-glucosidase inhibitory constituents from Duranta repens. Chem Pharm Bull (Tokyo) 2004;52:785-9.

Ijaz F, Ahmad N, Ahmad I, Haq AU, Wang F. Two new anti-plasmodial flavonoid glycosides from Duranta repens. J Enzym Inhib Med Chem 2010;25:773-8.

Vivar JL, Reyes MM, Shen CC, Ragasa CY. Chemical constituents of Duranta erecta L. Flowers. Pharm Lett 2016;8:234-6.

Ahmed WS, Mohamed MA, El-Dib RA, Hamed MM. New triterpene saponins from Duranta repens Linn. and their cytotoxic activity. Molecules 2009;14:1952-65.

Ijaz F, Haq A, Ahmad I, Ahmad N, Hussain J, Chen S. Antioxidative iridoid glycosides from the sky flower (Duranta repens Linn). J Enzym Inhib Med Chem 2011;26:88-92.

Kuo YH, Chen ZS, Lin YL. Chemical components of the leaves of Duranta repens Linn. Chem Pharm Bull 1996;44:429-36.

Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: An overview. Int J Chem Stud 2020;8:603-8.

Januar SE, Sugita P, Arifin B. Identification of trans-cinnamic acid in sinyo nakal (Duranta repens) fruits’ methanol extract. Int Res J Pure Appl 2015;8:73-80.

Donkor S, Larbie C, Komlaga G, Emikpe BO. Phytochemical, antimicrobial, and antioxidant profiles of Duranta erecta L. Parts. Biochem Res Int 2019;2019:8731595.

Agawane SB, Gupta VS, Kulkarni MJ, Bhattacharya AK, Koratkar SS, Rao VK. Patho-physiological evaluation of Duranta erecta for the treatment of urolithiasis. J Ayurveda Integr Med 2017;30:1-8.

Agwu EJ, Odo EG, Ekeh F, Uwagbae M, Ngwu G, Ehilegbu C. Bioefficacy of Duranta erecta Leaf extract on yellow fever and dengue vector, Aedes aegypti Linn. In Nigeria. J Med Plants Res 2018;12:124-32.

Johnson E, Udoh A, Etim E, Attih E, Williams I. Phytochemicals studies, proximate and mineral composition of ethanol leaf extract of Duranta repens Linn (Verbenaceae). Res J Phytochem 2018;12:1-6.

Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJ. Factors affecting secondary metabolite production in plants: Volatile components and essential oils. Flavour Fragr J 2008;23:213-26.

Fu Z, Chen R. Study of complexes of tannic acid with Fe (III) and Fe (II). J Anal Methods Chem 2019;2019:3894571.

Katavic PL. Chemical Investigations of the Alkaloids from the Plants of the Family Elaeocarpaceae. Queensland, Australia: Griffith university; 2006. p. 59-60.

Tiwari PK, Kaur M, Kaur H. Phytochemical screening and extraction: A review. Int Pharm Sci 2011;1:98-106.

Coe FG, Anderson GJ. Screening of medicinal plants used by the Garifuna of Eastern Nicaragua for bioactive compounds. J Ethnopharmacol 1996;53:29-50.

Moghimipour E, Handali S. Saponin: Properties, methods of evaluation and applications. Ann Res Rev Biol 2015;5:207-20.

Adu JK, Amengor CD, Kabiri N, Orman E, Patamia SA, Okrah BK. Validation of a simple and robust liebermann-burchard colorimetric method for the assay of cholesterol in selected milk products in Ghana. Int J Food Sci 2019;2019:9045938.

Published

07-08-2020

How to Cite

SUGITA, P., R. AMILIA, B. ARIFIN, D. U. C. RAHAYU, and H. DIANHAR. “THE PHYTOCHEMICAL SCREENING HEXANE AND METHANOL EXTRACT OF SINYO NAKAL (DURANTA REPENS)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 8, Aug. 2020, pp. 196-00, doi:10.22159/ajpcr.2020.v13i8.38165.

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