A SYNERGISTIC INSULINOTROPIC EFFECT OF GREEN TEA EXTRACT AND POMEGRANATE EXTRACT WITH ROSIGLITAZONE: BOTH IN VIVO AND IN VITRO EXPERIMENT
Objective: Scientists have growing interest in traditional medicinal plants as they contain active ingredients for the treatment of various diseases. Tea is one of the most popular beverages worldwide. The variety of tea and tea extracts in the market has different polyphenol profiles, which are the bioactive chemical entities. We performed a direct comparison between Thea sinensis, green tea extracts (GTEs), and Punica granatum peel powder (PGPP), which have been chemically well characterized in a type II diabetic mouse model.
Methods: We conducted both in vivo and in vitro experiments in the present paper. In vivo studies were carried out on male Swiss albino rats having type II diabetes, induced by single intravenous injection of streptozotocin (0.7 mg/Kg i.m.) and IDDM rats received either PGPP (200 mg/kg) or GTE (100 mg/kg) as a single oral dose. After the above result, the extracts were further subjected to know the effect of insulin secretion by RIN-5F cells providing confirmation of insulinotropic effect.
Results: The results revealed that both PGPP and GTE substantially lowered blood glucose levels and ameliorated glucose intolerance, both were effective in antihyperglycemic activity and in lowering body weight gain. Serum insulin levels significantly increased in GTE group as well as in PGPP group, suggesting that they were exerting hypoglycemic effects through different pathways.
Conclusion: Synergistic action of PGPP and GTE is an effective alternative for the treatment of type II diabetes through the regeneration of β cells of pancreas.
2. Salim KS. Hypoglycemic property of ginger and green tea and their possible mechanisms in diabetes mellitus. Open Conf Proc J 2014;5:13-9.
3. Fu QY, Li QS, Lin XM, Qiao RY, Yang R, Li XM, et al. Antidiabetic effects of tea. Molecules 2017;22:849-58.
4. Baliga MS, Shivashankara AR, Shetty CB, Thilakchand KR, Periera N, Palatty PL. Antidiabetic effects of Punica granatum (pomegranate): A review. In: Watson RR, Preedy V, editors. Bioactive Food as Dietary Interventions for Diabetes. 1st ed. New York: Academic Press; 2013. p. 355-69.
5. Tanga W, Lia S, Liub Y, Huang MT, Ho CT. Anti-diabetic activity of chemically profiled green tea and black tea extracts in a Type 2 diabetes mice model via different mechanisms. J Func Foods 2013;5:1784-93.
6. Jain A, Pramodkumar PG. In silico comparative molecular docking study and analysis of glycyrrhizin from Abrus precatorius (L.) against antidiabetic activity. Eur J Med Plants 2015;6:212-22.
7. Saad EA, Hassanien MM, El-hagrasy MA, Radwan KH. Antidiabetic, hypolipidemic and antioxidant activities and protective effects of punica granatum peels powder against pancreatic and hepatic tissues injuries in streptozotocin induced iddm in rats. Int J Pharm Pharm Sci 2015;7:397-402.
8. Cheng KH, Fu YC, Rong SL, Moon JY. iGEMDOCK: A graphical environment of enhancing GEMDOCK using pharmacological interactions and post-screening analysis. BMC Bioinformatics 2011;12:33-6.
9. Hassan Z, Yam MF, Ahmad M, Yusof AP. Antidiabetic properties and mechanism of action of Gynura procumbens water extract in streptozotocin-induced diabetic rats. Molecules 2010;15:908-23.
10. Rekha S, Chandrashekhara S. In silico proportional molecular docking study and analysis of insulinotropic activity of TZD derivatives by PPAR? activation. J Pharm Sci Res 2017;9:1799-808.
11. Anindita B, Bithin M, Sandip M, Kaushik C, Tapan S. In-vitro anti-diabetic and anti-oxidant activities of ethanol extract of Tinospora sinesis. Int J Curr Pharm Res 2017;9:379-84.
12. Ahmed AT, Belal SK, Salem AG. Protective effect of pomegranate peel extract against diabetic-induced renal pathological histo-changes in albino rats. IOSR JDMS 2014;13:94-105.
13. David AN, Welsh M, Casadaban MJ, Steiner DF. Control of insulin gene expression in pancreatic ?-cells and in an insulin-producing cell line, RIN-5F Cells. J Biol Chem 1985;260:13585-9.
14. Al Nahdi AM, Annie J, Haider R. Elucidation of molecular mechanisms of streptozotocin-induced oxidative stress, apoptosis, and mitochondrial dysfunction in rin-5F pancreatic ?-cells. Hind Oxid Med Cell Long 2017;2017:7054272.
15. Parka JH, Choa KI, Namb H, Choec NH, Suh JG. Anti-apoptotic effects of silk fibroin hydrolysate in RIN5F cell on high glucose condition. Anim Cells Sys 2015;19:201-7.
16. Sepehr KS, Baradaran B, Mazandarani M, Khori V, Shahneh FZ. Studies on the cytotoxic activities of Punica granatum L. var. Spinosa (apple Punice) extract on prostate cell line by induction of apoptosis. Int Sch Res Net 2012;2012:547942.
17. Polychronopoulos E, Zeimbekis A, Kastorini CM, Papairakleous N, Vlachou I, Bountziouka V, et al. Effect of black and green tea consumption on blood glucose levels in non-obese elderly men and women from mediterranean islands (MEDIS epidemiological study). Eur J Nutr 2008;47:10-6.
18. Kuttan R. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. J Ethnopharmacol 2002;83:109-16.
19. Moghaddam G, Sharifzadeh M, Hassanzadeh G, Khanavi M, Hajimahmoodi M. Anti-ulcerogenic activity of the pomegranate peel (Punica granatum) methanol extract. Food Nut Sci 2013;4:43-8.
20. Nogueira DG, Pereira NA. Effect of an alcoholic extract from the pericarp of roma (Punica grantum L.) on insulin hypoglycaemia mice. Rev Bras Farm 1986;67:59-62.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.