BIO-PROSPECTING OF CLEISTANTHUS COLLINUS AND ITS ANTIBACTERIAL ACTIVITY

Authors

  • Suman.T
  • R. Elangomathavan

Keywords:

Cleistanthus collinus, Phytochemistry, Bioprospecting, Antibacterial activity, MIC, MBC

Abstract

Objective: This present study was planned to resolve the phytochemical profile of the plant leaf extracts prepared by two different (hot and cold) extraction methods and to determine the bactericidal efficiency of Cleistanthus collinus.

Methods: Preliminary phytochemical analysis of C. collinus leaf crude extracts (methanol, ethanol, ethyl acetate and aqueous) were performed according to the Odebiyi method. The bactericidal efficiency of C. collinus leaf crude extracts were tested by standard microbiological methods against Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus, Listeria monocytogenes (Gram-positive).

Results: The presence of secondary metabolites like alkaloids, flavonoids, steroids, terpenoids, tannins, phlobatannins, glycosides and saponins were observed in preliminary phytochemical analysis of C. collinus leaf crude extracts. Analysis of bactericidal efficiency for the various solvent based extracts revealed that methanol extract of C. collinus was found to be intensive antibacterial activity [> 18 mm inhibition zone; MIC (0.27 - 0.42 mg/ml); MBC (0.35 – 0.50 mg/ml)] which is followed by ethanol extract and other extracts.  

Conclusion: From this study, it is obvious that the C. collinus leaf crude extract could be exploited for the preparation of new and efficient green medicine to treat various infectious diseases for both human being and veterinary animals.

Keywords: Cleistanthus collinus, Phytochemistry, Bioprospecting, Antibacterial activity, MIC, MBC.

References

Cowan MM. Plant products as anti-microbial agents. Clinical microbiology reviews 1999; 12:564-82.

Subramanian R, Krishnamurthy G. Thin-layer chromatographic detection of the lignin lactones of Cleistanthus collinus (Roxb.). J Chromatogr 1975; 107: 230-3.

Annapoorani KS, Periakali P, Illangovan S, Damodaran C, Chabdrasekharan P. Spectrofluorometric Determination of the Toxic constituents of Cleistanthus collinus. J Anal Toxicol 1984; 8:182-6.

Eswarappa S, Chakraborty AR, Palatty BU, Vasnaik M. Cleistanthus collinus poisoning: case report and review of the literature. J Toxicol 2003; 41:369-72.

Nandakumar NV, Pagala MKD, Venkataciiari SAT, Namba T, Grob D. Effect of Cleistanthus collinus leaf extract on neuromuscular function of the isolated mouse phrenic nerve-diaphragm. Toxicon 1989; 27:1219-28.

Pradheep Kumar CP, Panneerselvam N, Rajesh S, Shanmugam G. Cytotoxic and genotoxic effects of cleistanthin B in normal and tumour cells. Mutagenesis 1996; 11: 543–547.

Bhakuni DS, Dhar ML, Dhar MM, Dhawan BN, Mehrota BN. Screening of Indian plants for biological activity part II. Indian Exp Bio 1969; 7: 20.

Maity S. Ethnobotany of Lateritic West Bengal. Vidyasagar University [Ph. D. Thesis.]. Midnapore. 2002.

Odebiyi OO, Sofowora EA. Phytochemical screening of Nigerian medicinal plants part II. Lloydia 1978; 41: 234-235.

Olutiola PO, Famurewa O. Sonntag HG. An Introduction to General Microbiology - a Practical Approach [2nd ed]. 1991.

Yoo SY, Park SJ, Jeong DK, Kim KW, Lim SH, Lee SH, et al. J Microbiol 2007; 45 (3):246–55.

Govindachari TR, Sathe SS, Viswanathan N, Pai BR, Srinivasan M. Chemical constituents of Cleistanthus collinus (Roxb.) Tetrahedron 1969; 25: 2815-2821.

Elmahmood AM, Amey JM. In vitro antibacterial activity of Parkia biglobosa (Jacq) root bark extract against some microorganisms associated with urinary infections. Afr J Biotechnol 2007; 6(11): 1272-1275.

Levin MD, Vandon-Berghe DA, Marten T, Vilientmick A, Lomwease EC. Screening of higher plants for biological activity. Planta Medica 1979; 36:311-312.

Elmahmood AM, Doughari JH. Phytochemical screening and antibacterial evaluation of the leaf and root extracts of Cassia alata L. Afr J Phar Pharmacol 2008; 2 (7): 124-129.

Mbuh FA, Asika IS, Doughari JH. Studies on antibacterial activities of leaf extracts of Psidium guajava L. Best J. 2008; 5(1): 44-47.

Maji S, Dandapat P, Ojha D, Maity C, Halder SK, Das Mohapatra PK, Pathak TK, Pati BR, Samanta A, Mondal KC. In vitro antimicrobial potentialities of different solvent extracts of ethnomedicinal plants against clinically isolated human pathogens. J Phyto 2010: 2(4): 57–64.

Parekh J, Chanda S. In vitro antimicrobial activity of Trapanatans Linn. Fruit rind extracted in different solvents. Afr J Biotechnol 2007; 6(6): 766-770.

Abhishek K, Tapan Kumar C. In vitro antimicrobial activity of thiophene derivative pitc-2 of Pluchea indica and its mechanism of action. Asian J Pharm Clin Res 2013; 6(2): 115-117.

Mukherjee PK, Wahile A. Integrated approaches towards drug development from Ayurveda and other Indian system of medicines. J Ethnopharmacol 2006; 103: 25- 35.

Shahina N, Samina A, Sheikh AR, Rahmanullah S, Syed AS. In vitro antibacterial activity of the extracts derived from Terminalia catappa. Res J Microbiol 2007; 2: 180-184.

Durga KR, Karthikumar S, Jegatheesan K. Isolation of potential antibacterial and antioxidant compounds from Acalypha indica and Ocimum basilicum. J Med Plant Res 2009; 3: 703-706.

Balakrishnan N, Alka S. Preliminary phytochemical and pharmacological activities of Luffa Cylindrica Fruit. Asian J Pharm Clin Res 2013; 6(2): 113-116.

Prescott LM, Harley PJ, Klein AD. Microbiology. McGraw Hill Publisher [7th ed] Singapore. 2008. p. 94-122.

Published

2013-09-01

How to Cite

Suman.T, and R. Elangomathavan. “BIO-PROSPECTING OF CLEISTANTHUS COLLINUS AND ITS ANTIBACTERIAL ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 8, Sept. 2013, pp. 206-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/390.

Issue

Section

Articles