• MD. MOHAIMENUL Department of Pharmacy, Jashore University of Science and Technology, Jashore, Bangladesh.
  • KATHY DUTTA Department of Pharmacy, Jashore University of Science and Technology, Jashore, Bangladesh.
  • NOWRIN FERDIOUSI Department of Pharmacy, Jashore University of Science and Technology, Jashore, Bangladesh.
  • DEBENDRA NATH ROY Department of Pharmacy, Jashore University of Science and Technology, Jashore, Bangladesh.


Objective: The present study was designed to evaluate and compare antidiabetic, analgesic, and cytotoxic properties of Lal shak (Amaranthus gangeticus L.) and Chanchi shak (Alternanthera sessilis L.). We carried out this work to explore the medicinal uses of very common and cheap leafy plant vegetables among the people of all classes.

Methods: The antidiabetic activity was evaluated and compared by studying the effect of ethanolic extract of A. gangeticus (EEAG) and ethanolic extract of A. sessilis (EEAS) against blood glucose level of alloxan-induced diabetic mice in every 6 h for 24 h. To evaluate and compare analgesic and cytotoxic activity, different tests such as acetic acid-induced writhing test, hot plate test, and brine shrimp lethality bioassay test had been performed.

Results: The mice were treated with both plants extract at a dose of 200 mg/kg body weight in case of antidiabetic activity test. Blood glucose level was examined and found that there was a significant reduction of blood glucose level with EEAG (p<0.05) and EEAS (p<0.001) in comparison with their respective diabetic control group. Although both plant extracts reduced the blood glucose level, the glucose reducing effect was higher in EEAS. Both the plants showed significant (p<0.05) peripheral analgesic activity in treated mice but no significant central analgesic activity. EEAG showed higher peripheral analgesic activity than EEAS. In brine shrimp lethality bioassay, both the plants showed higher LC50 value thus cytotoxicity occurs at very higher dose and safe to administer.

Conclusion: In this study, both the plants showed sufficient antidiabetic property and higher LC50 value, thus administration of leafy vegetable Lal shak and Chanchi shak may be useful for diabetic people. Chanchi shak may be more helpful for diabetic people than Lal shak.

Keywords: Amaranthus gangeticus L., Alternanthera sessilis L., Antidiabetic, Analgesic, Cytotoxic


1. Tamilselvan N, Thirumalai T, Elumalai EK, Balaji R, David E. Pharmacognosy of Coccinia grandis: A review. Asi Pac J Trop Biomed 2011;1:S299-302.
2. Dias JS. Nutritional quality and health benefits of vegetables: A review. Food Nutr Sci 2012;3:1354-74.
3. Jerz G, Arrey TN, Wray V, Du Q, Winterhalter P. Structural characterization of 132-hydroxy-(132-S)-phaeophytin-a from leaves and stems of Amaranthus tricolor isolated by high-speed countercurrent chromatography. Innovative Food Sci Emerg Technol 2007;8:413-8.
4. Anjali K, Joshi A, Maloo SR, Sharma R. Assessment of the morphological and molecular diversity in Amaranthus spp. Afr J Agric Res 2013;8:2307-11.
5. Gupta RS, Sharma R. A Review on Medicinal Plants Exhibiting Antifertility Activity in Males. New Delhi: ICMR; 2004. p. 151-7.
6. Jalalpure SS, Agrawal N, Patil MB, Chimkode R, Tripathi A. Antimicrobial and wound healing activities of leaves of Alternanthera sessilis Linn. Int J Green Pharm 2008;2:141-4.
7. Kumar A, Das M, Mohanraj P, Das A. Antimicrobial activity study of ethanolic extract of Alternanthera sessilis Linn. Aerial parts. J Appl Pharm Res 2014;2:1-4.
8. Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: A model for Type 2 diabetes and pharmacological screening. Pharmacol Res 2005;52:313-20.
9. Lanjhiyana S, Garabadu D, Ahirwar D, Bigoniya P. Antidiabetic activity of methanolic extract of stem bark of Elaeodendron glaucum Pers. In alloxanized rat model. Adv Appl Sci Res 2011;2:47-62.
10. Naher S, Aziz MA, Akter MI, Rahman SM, Sajon SR. Analgesic, anti-inflammatory and anti-pyretic activities of methanolic extract of Cordyline fruticosa (L.) A. Chev Leaves J Res Pharm 2019;23:198-207.
11. Khalighi-Sigaroodi F, Ahvazi M, Hadjiakhoondi A, Taghizadeh M, Yazdani D, Khalighi-Sigaroodi S, et al. Cytotoxicity and antioxidant activity of 23 plant species of leguminosae family. Iran J Pharm Res 2012;11:295.
12. Federiuk IF, Casey HM, Quinn MJ, Wood MD, Ward KW. Induction of Type-1 diabetes mellitus in laboratory rats by use of alloxan: Route of administration, pitfalls, and insulin treatment. Comp Med 2004;54:252-7.
13. Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Fed Proc 1959;18:412-8.
14. Turner RA. Analgesics, in Screening Methods in Pharmacology. London, UK: Academic Press; 1965. p. 100-17.
15. Alonso-Castro AJ, Zavala-Sánchez MA, Pérez-Ramos J, Sánchez- Mendoza E, Pérez-Gutiérrez S. Antinociceptive and anti-arthritic effects of kramecyne. Life Sci 2015;121:70-7.
16. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DJ, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med 1982;45:31-4.
17. Deshpande AD, Harris-Hayes M, Schootman M. Epidemiology of diabetes and diabetes-related complications. Phys Ther 2008;88:1254-64.
18. Hossain AI, Faisal M, Rahman S, Jahan R, Rahmatullah M. A preliminary evaluation of antihyperglycemic and analgesic activity of Alternanthera sessilis aerial parts. BMC Complement Alternat Med 2014;14:169.
19. Ramachandran A, Snehalatha C, Ma RC. Diabetes in South-East Asia: An update. Diabetes Res Clin Pract 2014;103:231-7.
20. Saquib N, Khanam MA, Saquib J, Anand S, Chertow GM, Barry M, et al. High prevalence of Type 2 diabetes among the urban middle class in Bangladesh. BMC Public Health 2013;13:1032.
21. Anand S, Khanam MA, Saquib J, Saquib N, Ahmed T, Alam DS, et al. High prevalence of chronic kidney disease in a community survey of urban Bangladeshis: A cross-sectional study. Global Health 2014;10:9.
22. Muhammad N, Saeed M, Khan H. Antipyretic, analgesic and anti-inflammatory activity of Viola betonicifolia whole plant. BMC Complementary Alternat Med 2012;12:59.
23. Ahmed S, Sultana M, Mohtasheem M, Hasan U, Azhar I. Analgesic and antiemetic activity of Cleome viscosa L. Pak J Bot 2011;43:119-22.
24. McLaughlin JL, Chang CJ, Smith DL. Simple Bench-top Bioassays (Brine Shrimp and Potato Discs) for the Discovery of Plant Antitumor Compounds: Review of Recent Progress. United States: In ACS Symposium Series; 1993.
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How to Cite
MOHAIMENUL, M., K. DUTTA, N. FERDIOUSI, and D. NATH ROY. “COMPARATIVE STUDIES ON ANTIDIABETIC, ANALGESIC, AND CYTOTOXIC EFFECT OF ETHANOLIC EXTRACTS OF AMARANTHUS GANGETICUS L. AND ALTERNANTHERA SESSILIS L.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 11, Nov. 2020, pp. 113-7, doi:10.22159/ajpcr.2020.v13i11.39232.
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