EVALUATION OF ANXIOLYTIC POTENTIAL OF VARIOUS EXTRACTS OF TAMARINDUS INDICA FLOWERS
Objective: The objective of this study was designed to gauge the antianxiety activity of varied extracts, namely, n-hexane, chloroform, ethyl acetate, and methanol of the flowers of Tamarindus indica using elevated plus maze (EPM) model in albino mice.
Methods: Coarsely powdered tamarind was powdered and subjected to exhaustive Soxhlet extraction using n-hexane, chloroform, ethyl acetate, and methanol. Solvents were recovered from all extracts using a rotary vacuum evaporator under reduced pressure. Albino mice have ministered orally with different doses of the extracts (i.e., 200 and 400 mg/kg) and behavior was observed on the EPM. The standard (positive control) employed in the study Diazepam (2 mg/kg, P.O).
Results: Results indicate that the methanol extract of T. indica flowers showed maximum and significant dose-dependent effect at 200 and 400 mg/kg on EPM, the results were just like the standard antianxiety agent diazepam (2 mg/kg). Locomotor activity evaluated with two different doses of T. indica (200 and 400 mg/kg) using actophotometer. The results were shown to be decreased in a dose dependent model compared to control.
Conclusion: The methanol extract shows that the presence of polyphenols could be liable for the anxiolytic potential of T. indica. Hence, this plant could also be used as a useful antianxiety agent.
2. Lader M, Morton S. Benzodiazepine problems. Br J Addict 1991;86:823-8.
3. Griffiths RR, Ator NA, Roache JD, Lamb RJ. abuse liability of triazolam: Experimental measurements in animals and humans. Psychopharmacol Ser 1987;3:83-7.
4. Kobayashi A, Adenan ML, Kajiyama SI, Kanzaki H, Kawazu K. A cytotoxic principle of Tamarindus indica, di-n-butyl malate and the structure-activity relationships of its analogs. J Biosci 1996;51:233-42.
5. Ferrara L. Antioxidant activity of Tamarindus indica L. Ingredienti Aliment 2005;4:13-5.
6. Kristensen M, Balslev H. Woody plants availability,perceptions and use among the Gourounsi in Burkina Faso. Biodivers Conserv 2003;12:1715-39.
7. Dagar JC, Singh G, Singh NT. Agroforestry crops evolution was carried with tamarind (Tamarindus indica), teak (Tectoma grandish) and maharukh (Ailanthus excelsa), and on reclaimed salt-affected soils. J Trop For Sci 1995;7:623-34.
8. Balakrishna V, Kumar DS, Lakshmi T. Tamarindus indica L. (Fabaceae): Extent of explored use in traditional medicine. Asian J Pharm Clin Res 2020;13:28-32.
9. Julia MF. Tamarind (Tamarindus indica L.) its Food, Medicinal and Industrial Uses. ???: Florida State Horticultural Society; 1958. p. 288.
10. Trease EG, Evans WC. Textbook of Pharmacognosy. 12th ed. Singapore: Alden Press; 1983.
11. OECD Guidelines for the Testing of Chemicals No. 423, Acute Oral Toxicity-Acute Toxic Class Method; 2001.
12. Paladini AC, Marder M, Viola H, Wolfman C, Wasowaki C, Medina JH. Flavonoids and the central nervous system: From forgotten factors to potent anxiolytic compounds. J Pharm Pharmacol 1999;51:519-26.
13. Halemani D. Evaluation of anti-anxiety activity of methanol extract of Aegle maemelos (Bael fruit tree) leaves in rats. IOSR J Dent Med Sci 2015;14:1-5.
14. Ashwani K, Jitender S, Anupam S. Comparative study of antianxiety activity of Argyreia speciosa Linn. (Roots), Caesalpinia digyna rottler (Roots), and Sphaeranthus indicus Linn. (Flowers). Int J Pharm Sci Res 2015;6:4226-9.
15. Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Clavo D, et al. Neuroactive flavonoids: New ligands for the benzodiazepine receptors. Phytomedicine 1997;5:235-43.
16. Kulkarni SK, Reddy DS.Testing of antianxiety agents by animal behaviour models. Methods Find Exp Clin Pharmacol 1996;18:219-40.
17. Wolfman C, Viola H, Paladini A, Dajas F, Medina JH. Possible anxiolytic effects of chrysin, a central benzodiazepine receptor ligand isolated from Passiflora coerulea. Pharmacol Biochem Behav 1994;47:1-4.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.