• Thangarajan Starlin
  • Velliyur Kanniappan Gopalakrishnan


Tylophora pauciflora, enzymatic antioxidants, Non-enzymatic antioxidants, Oxidative stress, Free radicals


Objectives: Many oxidative stress related diseases are as a result of accumulation of free radicals in the body. A lot of researches are going on worldwide directed towards finding natural antioxidants of plants origins. In this study, we assessed enzymatic and non-enzymatic antioxidant properties of Tylophora pauciflora.

Method: The enzymatic antioxidants (Superoxide dismutase, Catalase, Glutathione-s-transferase, Glutathione peroxidase, Peroxidase, Ascorbate oxidase and Polyphenoloxidase) and non-enzymatic antioxidants (Total reduced glutathione and Vitamin C) activities were determined.

Results: The present study revealed that Tylophora pauciflora has an excellent source of enzymatic and non-enzymatic antioxidants.

Conclusion: The present study, results has capability to scavenge the free radicals and protect against oxidative stress causing diseases. In future Tylophora pauciflora may serve as a good pharmacotherapeutic agent.

Keywords: Tylophora pauciflora, enzymatic antioxidants, Non-enzymatic antioxidants, Oxidative stress, Free radicals


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Agarwal A, Prabakaran S, Allamaneni S. What an andrologist/urologist should know about free radicals and why. Urology.2006; 67: 2.

Panchawat S, Sisodia SS. In- vitro antioxidant activity of Saraca asoca Roxb. De wilde stem bark extracts from various extraction processes. Asian J Pharm Clin Res. 2010; 3: 231- 233

Agarwal ACM, Abdelrazik H, Sharma RK. Oxidative stress management in patients with male or female factor infertility. Handbook of Chemiluminescent methods in oxidative stress Assessment.2008; 195.

Kothari S, Thompson A, Agarwal, Plessia SS. Free radicals: Their beneficial and detrimental effects on sperm function. Indian J Exp Biol 2010; 48: 425-435.

Cheeseman KH, Slater TF. Free radicals in medicine. Brit Med Bullet. 1993; 49: 479-724.

Cowan MM, Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12: 564-582.

Thevasundari S, Rajendran A. Antibacterial Potential and Phytochemical analysis of Heterostemma tanjorense (WIGHT AND ARN). World J Sci Technol. 2011; 1suppl 11: 39-45.

Arunkumar S, Muthuselvam M. Analysis of phytochemical constituents and antimicrobial activities of Aloe vera (L). against clinical pathogens. World J Agr Sci 2009; 5:572-576.

Starlin T, ArulRaj C, Ragavendran P, Gopalakrishnan VK. Phytochemical screening, functional groups and elemental analysis of Tylophora pauciflora Wight and Arn. Int Res J Pharmacy. 2012; 3: 180-183.

Das K, Samanta L, Chainy GBN. A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radicals. Ind J Biochem Biophys. 2000; 37: 201-204.

Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972; 47: 389-394.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science. 1973; 179: 588-590.

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-Transferases: The First Enzymatic Step in Mercapturic Acid Formation. J Biol Chem 1974; 249: 7130.

Addy SK, Goodman RN. Polyphenol oxidase and peroxidase in apple leaves inoculated with a virulent or an avirulent strain for Ervinia amylovora. Ind Phytopath. 1972; 25: 575-579.

Vines HM, Oberbacher MF. Response of oxidation and phosphorylation in citrus mitochondria to arsenate. Nature. 1965; 206: 319-320.

Sadasivam S, Manickam A. Biochemical method: Polyphenol oxidase. New Age International (P) Limited, New Delhi. 1996; 2, 108-110.

Boyne AF, Ellman GL. A methodology for analysis of tissue sulfhydryl components. Anal Biochem. 1972; 46: 639-53.

Ragavendran P, Arul Raj C, Sophia D, Starlin T, Gopalakrishnan VK. Evaluation of Enzymatic and Non-Enzymatic Antioxidant Properties of Aerva Lanata (L) - An In vitro Study. Int J Pharm Pharm Sci. 2012; 1: 4, 522-526.

Lee YL, Yen MT, Mau JL. Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chem., 2007; 104: 1-9.

Hakiman M, Maziah M, Non enzymatic and enzymatic antioxidant activities in aqueous extract of different Ficus deltoidea accessions. J. Med. Plants Res. 2009; 3 suppl 3: 120-131.

Johnson SM, Doherty SJ, Croy RRD, Biphasic superoxide generation in potato tubers: a self amplifying response to stress. Plant Physiol. 2003; 13: 1440-1449.

Gout E, Boisson AM, Aubert S, Douce R, Bligny R. Origin of the cytoplasmic pH changes during anaerobic stress in higher plant cells. Carbon-13 and phosphorus-31 nuclear magnetic resonance studies. Plant Physiology. 2001; 100: 912-925.

Kusvuran S, Ellialtioglu S, Yasar S, Abak K. Antioxidative enzyme activities in the leaves and callus tissues of salt-tolerant and salt-susceptible melon varieties under salinity. African Journal of Biotechnology. 2012; 11suppl 3: 635-641.

Curtis B, Moritz M, Snodgrass PJ. Serum enzymes derived from liver cell fraction and the response to carbon tetrachloride intoxication in rats. Gastroenterology. 1972; 62: 84-92

Scandalios JG. The antioxidant enzyme genes CAT and SOD of maize: regulation, functional significance and molecular biology. Isozymes Curr. Top. Biol. Med. Res. 1987; 14: 19-44.

Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002; 7: 405-410.

Sarvajeet SG, Narendra T. Reactive oxygen species and antioxidant machinery in a biotic stress tolerance in crop plants. Annual Review. Plant Physiology and Biochemistry. 2010 3: 1-22.

Solomon EI, Sundaram UM, Machonkin TE. Multicopper oxidases and oxygenases. Chem. Rev., 1996; 96: 2563-2605.

Weis W. Ascorbic acid and electron transport. Ann NY Acad Sci. 1975; 258: 190-200.

Michiels C, Raes M, Toussaaint O, Remacle J. Importance of selenium glutathione peroxidase, catalase and Cu/Zn-SOD for cell survival against oxidative stress. Free Radical Bio. Med. 1994; 17: 235-248.

Gomathi D, Kalaiselvi M, Ravikumar G,Uma C. Evaluation of enzymatic and non-enzymatic antioxidant potential of Evolvulus alsinoides (L.)L. Asian J Pharm Clin Res. 2012; 5: Suppl 2: 159-163

Pric A, Lucas PW, Lea DJ. Age dependent damage and glutathione metabolism in ozone fumigated barley: A leaf section approach. J. Exp. Bot. 1990; 41: 1309-1317.

Ruegesgger A, Schmutzz D, Brund C. Regulation of glutathione biosynthesis by cadmium in Pisum sativum L. Plant Physiol., 1990 93: 1579-1584.

Kayang H, Tribal knowledge on wild edible plants of Meghala Indian A, Northeast India. J. Traditional Knowledge. 2007; 6 suppl 1: 177-181

Maxwell SRJ. Prospects for use of antioxidant therapies. Drugs.1995; 49: 345-361.

Sies H. Strategies of antioxidant defence. Eur. J. Biochem. 1993; 215: 213-219.

Jacob RA. Passive smoking induces oxidant damage preventable by vitamin C. Nutr. Rev. 2000; 58: 239-241.

Kumar R, Hemalatha S. In-vitro antioxidant activity of alcoholic leaf extract and sub fractions of Alangium lamarckii Thwaites. J. Chem. Pharm. Res. 2011; 3 suppl 1: 259-267.



How to Cite

Starlin, T., and V. K. Gopalakrishnan. “ENZYMATIC AND NON-ENZYMATIC ANTIOXIDANT PROPERTIES OF Tylophora Pauciflora Wight and Arn. – AN IN VITRO STUDY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 8, Sept. 2013, pp. 68-71, https://innovareacademics.in/journals/index.php/ajpcr/article/view/393.