• MOUNA BOUARFA Laboratory of Neuroendocrinology and Nutritional and Climatic Environment. Faculty of Sciences, Sidi Mohamed Ben Abdellah University, B.P. 1796, Atlas, Fez, Morocco.
  • ZOUHAIR SOUIRTI CHU Hassan II - Neurology - 30000 - Fez – Morocco.
  • PIERFRANCESCO CERRUTI 3Institute for Polymers, Composites and Biomaterials (IPCB-CNR), Naples, Italy.
  • MAURIZIO AVELLA Institute for Chemistry and Technology of Polymers (ICTP-CNR), Naples, Italy.
  • ABDELLAH FARAH Laboratory of Applied Organic Chemistry, Faculty of Sciences and Technology, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
  • DALILA BOUSTA Laboratory of Neuroendocrinology and Nutritional and Climatic Environment. Faculty of Sciences, Sidi Mohamed Ben Abdellah University, B.P. 1796, Atlas, Fez, Morocco.


Objective: The objective of the present study was to evaluate the anti-obesity activity of Cannabis sativa seed Flour (CSSF) on obese mice.

Methods: The experiment process was carried out in 8 weeks. In this time, three groups of animals were adopted: High caloric diet control group (HCD), group treated with 100 mg/kg of CSSF, group treated with 200 mg/kg of CSSF, and normal diet control group (ND). Then, the histopathological and biochemical parameters analysis has been realized at the end of the experiment.

Results: As a result, body weight gain, epididymal, and perirenal adipose tissue decreased in the CSSF (Cannabis sativa seed Flour) group in both doses (100 mg/kg and 200 mg/kg) compared to the HCD. CSSF lowered serum glucose level elevated by HCD. For lipid profiles, total cholesterol (TC), HDL-cholesterol, LDL-cholesterol, and triglycerides (TG) significantly decreased in the CSSF group. The histopathological results of this study showed a regenerative effect of CSSF on the kidney and liver tissues.

Conclusion: It is concluded from the present study that CSSF can be used as a natural anti-obesity agent.

Keywords: Cannabis sativa seed flour, Anti-obesity, Mice, Kidney, Liver, Taounate, Kif, Khlalfa


1. de Padua LS, Bunyaprafatsara N, Lemmens RH. Plant Resources of South-East Asia: Medicinal and Poisonous Plants. Leiden: Backhuys Publishers; 1999. p. 167-75.
2. Jiang HE, Li X, Zhao YX, Ferguson DK, Hueber F, Bera S, et al. A new insight into Cannabis sativa (Cannabaceae) utilization from 2500-year-old Yanghai Tombs, Xinjiang, China. J Ethnopharmacol 2006;108:414-22.
3. Callaway JC. Hempseed as a nutritional resource: An overview. Euphytica 2004;140:65-72.
4. Chuan-He T, Zi T, Xian-Sheng W, Xiao-Quan Y. Physicochemical and functional properties of hemp (Cannabis sativa L.) protein isolate. J Agric Food Chem 2006;54:8945-50.
5. Odani S, Odani S. Isolation and primary structure of a methionine and cystine-rich seed protein of Cannabis sativa L. Biosci Biotechnol Biochem 1998;62:650-4.
6. Callaway J, Pate D. Hempseed oil. In: Moreau R, Kamal-Eldin A, editors. Gourmet and Health-promoting Specialty Oils. 1st ed. Urbana: AOCS Press; 2008. p. 215-36.
7. Shou-Wei Y, Chuan-He T, Qi-Biao W, Xiao-Quan Y. Properties of cast films from hemp (Cannabis sativa L.) and soy protein isolates. A comparative study. J Agric Food Chem 2007;55:7399-404.
8. Collin P. New diagnostic findings in coeliac disease. Ann Med 1999;31:399-405.
9. Claudine L. Guide Phyto-Minceur: Comment Maigrir à L’Aide des Plantes. 1st ed. France: Dangles; 2012.
10. Ministère de la Santé, UNICEF. La Stratégie Nationale de la Nutrition 2011-2019. Maroc: Ministère de la Santé, UNICEF; 2011.
11. del Prete A, Iadevaia M, Loguercio C. The role of gut hormones in controlling the food intake. What is their role in emerging diseases? Endocrinol Nutr 2012;59:197-206.
12. Gamboa-Gómez CI, Rocha-Guzmán NE, Gallegos-Infante JA, Moreno- Jiménez MR, Vázquez-Cabral BD, González-Laredo RF. Plants with potential use on obesity and its complications. EXCLI J 2015;14:809-31.
13. Javid M, Archana P. Evaluation of the anti-obesity effect of aqueous extract of Mucuna pruriens seeds on rats. Int J Pharm Pharm Sci 2017;9:111-5.
14. Adnyana IK, Sukandar EY, Yuniarto A, Finna S. Anti-obesity effect of the pomegranate leaves ethanol extract (Punica granatum L.) in high fat diet induced mice. Int J Pharm Pharm Sci 2014;6:626-31.
15. Athesh K, Divakar M, Brindha P. Anti-obesity potential of Cyperus rotundus L. aqueous tuber extract in rats fed on high-fat cafeteria diet. Asian J Pharm Clin Res 2014;7:88-92.
16. Nan-Nong S, Tsung-Yen W, Chi-Fai C. Natural dietary and herbal products in anti-obesity treatment. Molecules 2016;21:1351.
17. Chandrasekaran CV, Vijayalakshmi MA, Prakash K, Bansal VS, Meenakshi J, Amit A. Review article: Herbal approach for obesity management. Am J Plant Sci 2012;3:1003-14.
18. Kaiyala KJ, Prigeon RL, Kahn SE, Woods SC, Porte DJ, Schwartz MW. Reduced beta-cell function contributes to impaired glucose tolerance in dogs made obese by high fat feeding. Am J Physiol Endocrinol Metab 1999;277:659-66.
19. Brown JL, Spicer MT, Spicer LJ. Effect of high-fat diet on body composition and hormone responses to glucose tolerance tests. Endocrine 2002;19:327-32.
20. Morell P, Fiszman S. Revisiting the role of protein-induced satiation and satiety. Food Hydrocoll 2017;68:199-210.
21. Bendtsen LQ, Lorenzen JK, Bendsen NT, Rasmussen C, Astrup A. Effect of dairy proteins on appetite, energy expenditure, body weight, and composition: A review of the evidence from controlled clinical trials. Adv Nutr 2013;4:418-38.
22. van der Klaauw A, Keogh J, Henning E, Trowse V, Dhillo W, Ghatei M. High protein intake stimulates postprandial GLP1 and PYY release. Obesity (Silver Spring) 2013;21:1602-7.
23. Slavin JL. Dietary fiber and body weight. Nutrition 2005;21:411-8.
24. Astrup A, Kristensen M, Gregersen NT, Belza A, Lorenzen JK, Due A, et al. Can bioactive foods affect obesity? Ann NY Acad Sci 2010;1190:25-41.
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How to Cite
BOUARFA, M., Z. SOUIRTI, P. CERRUTI, M. AVELLA, A. FARAH, and D. BOUSTA. “ANTI-OBESITY EFFECT OF CANNABIS SATIVA SEED FLOUR FROM KHLALFA OF TAOUNATE REGION (NORTHERN OF MOROCCO) IN HIGH CALORIC DIET-INDUCED OBESE MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 11, Sept. 2020, pp. 139-44, doi:10.22159/ajpcr.2020.v13i11.39304.
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