• RAMDAS N KALE Department of Pharmacognosy, SVPM’s College of Pharmacy, Baramati, Pune, India.
  • RAVINDRA Y PATIL Department of Pharmacognosy, PDEA’s Shankarrao Ursal College of Pharmaceutical Sciences and Research Center, Kharadi, Pune, India.


Objective: The objective of the study was to evaluate the effect of Piper betle L. leaves extract against clonidine-induced catalepsy and milk-induced leukocytosis and eosinophilia in mice.

Methods: Methanolic extract of P. betle L. leaves was prepared using Soxhlet apparatus. Preliminary phytochemical screening of the prepared extract was carried out using standard chemical tests. Effect of the prepared extract was evaluated against clonidine-induced catalepsy and milk-induced leukocytosis and eosinophilia in mice model.

Results: Maximum duration of catalepsy was observed at 90 min after the clonidine administration. There was a significant inhibition (p˂0.05) of clonidine-induced catalepsy in the animals pretreated with chlorpheniramine maleate and extract of P. betle leaves. Administration of milk (4 mg/kg) subcutaneous route exhibited a significant increase in leucocytes and eosinophil count after 24 h of administration. Methanolic extract of P. betle L. leaves showed significant inhibition (p˂0.05) of milk-induced leukocytosis and eosinophilia.

Conclusion: These results suggest that P. betle leaves extract may have the potential therapeutic value in the treatment of allergic diseases.

Keywords: Catalepsy, Leukocytosis, Eosinophilia


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How to Cite
N KALE, R., and R. Y PATIL. “EVALUATION OF THE EFFECT OF PIPER BETLE L. LEAVES EXTRACT AGAINST CLONIDINE-INDUCED CATALEPSY AND MILK-INDUCED LEUKOCYTOSIS AND EOSINOPHILIA IN MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 11, Nov. 2020, pp. 118-21, doi:10.22159/ajpcr.2020.v13i11.39321.
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