EVALUATION OF THE EFFECT OF PIPER BETLE L. LEAVES EXTRACT AGAINST CLONIDINE-INDUCED CATALEPSY AND MILK-INDUCED LEUKOCYTOSIS AND EOSINOPHILIA IN MICE
Objective: The objective of the study was to evaluate the effect of Piper betle L. leaves extract against clonidine-induced catalepsy and milk-induced leukocytosis and eosinophilia in mice.
Methods: Methanolic extract of P. betle L. leaves was prepared using Soxhlet apparatus. Preliminary phytochemical screening of the prepared extract was carried out using standard chemical tests. Effect of the prepared extract was evaluated against clonidine-induced catalepsy and milk-induced leukocytosis and eosinophilia in mice model.
Results: Maximum duration of catalepsy was observed at 90 min after the clonidine administration. There was a significant inhibition (p˂0.05) of clonidine-induced catalepsy in the animals pretreated with chlorpheniramine maleate and extract of P. betle leaves. Administration of milk (4 mg/kg) subcutaneous route exhibited a significant increase in leucocytes and eosinophil count after 24 h of administration. Methanolic extract of P. betle L. leaves showed significant inhibition (p˂0.05) of milk-induced leukocytosis and eosinophilia.
Conclusion: These results suggest that P. betle leaves extract may have the potential therapeutic value in the treatment of allergic diseases.
2. Kumar N, Misra P, Dube A, Bhattacharya S, Dikshit M, Ranade S. Piper betle Linn. a maligned Pan-Asiatic plant with an array of pharmacological activities and prospects for drug discovery. Curr Sci 2010;99:922-32.
3. Bhattacharya S, Banerjee D, Bauri AK, Chattopadhyay S, Bandyopadhyay SK. Healing property of the Piper betel phenol, allylpyrocatechol against indomethacin-induced stomach ulceration and mechanism of action. World J Gastroenterol 2007;13:3705-13.
4. Sapna S, Anju D, Sanju N. Pharmacognostical and phytochemical studies of piper betle Linn leaf. Int J Pharm Pharm Sci 2016;8:222-6.
5. Gundala SR, Aneja R. Piper betel leaf: A reservoir of potential xenohormetic nutraceuticals with cancer-fighting properties. Cancer Prev Res (Phila) 2014;7:477-86.
6. Arif B, Risris K, Tazyinul QA. Wound-healing test of piper betle leaf extract and Aloe vera in gel preparation. Int J Appl Pharm 2018;10:86-91.
7. Marlia S, Sophi D, Natalia P. Antimicrobial activity of standardized piper betel extract and its mouthwash preparation. Int J Pharm Pharm Sci 2014;6:243-6.
8. Agarwal T, Singh R, Shukla AD, Waris I, Gujrati A. Comparative analysis of antibacterial activity of four Piper betel varieties. Adv Appl Sci Res 2012;3:698-705.
9. Jaiswal SG, Patel M, Saxena DK, Naik SN. Antioxidant properties of Piper betel (L) leaf extracts from six different geographical domain of India. J Bioresour Eng Technol 2014;2:12-20.
10. Majumdar B, Chaudhuri SG, Ray A, Bandyopadhyay SK. Effect of ethanol extract of Piper betle Linn leaf on healing of NSAID-induced experimental ulcer--a novel role of free radical scavenging action. Indian J Exp Biol 2003;41:311-5.
11. Chan EW, Wong SK. Phytochemistry and pharmacology of three Piper species: An update. Int J Pharmacogn 2014;1:534-44.
12. Singh M, Shakya S, Soni VK, Dangi A, Kumar N, Bhattacharya SM. The n-hexane and chloroform fractions of Piper betle L. trigger different arms of immune responses in BALB/c mice and exhibit antifilarial activity against human lymphatic filarid Brugia malayi. Int Immunopharmacol 2009;9:716-28.
13. Pal M, Chandrashekar K. Mosquito repellent activity of Piper betel Linn. Int J Pharm Life Sci 2010;1:313-5.
14. Alam B, Akter F, Parvin N, Sharmin Pia R, Akter S, Chowdhury J, et al. Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna J Phytomed 2013;3:112-25.
15. Sarma C, Rasane P, Kaur S, Singh J, Singh J, Gat Y, et al. Antioxidant and antimicrobial potential of selected varieties of Piper betle L. (Betel leaf). An Acad Bras Cienc 2018;90:3871-8.
16. Fathima Begam KM, Ravichandran P, Manimekalai V. Phytochemical analysis of some selected varieties of piper betle L. Int J Curr Pharm Res 2018;10:89-93.
17. Khandelwal KR. Practical Pharmacognosy Technique and Experiments. 23rd ed. Pune: Nirali Prakashan; 2005. p. 15-29.
18. National Research Council. Guide for the Care and Use of Laboratory Animals. Washington, DC: Institute for Laboratory Animal Research, National Academics Press; 2011. p. 11-40.
19. Ferré S, Guix T, Prat G, Jane F, Casas M. Is experimental catalepsy properly measured? Pharmacol Biochem Behav 1990;35:753-7.
20. Taur DJ, Nirmal SA, Patil RY. Effect of various extracts of Ficus bengalensis bark on clonidine and haloperidol-induced catalepsy in mice. Pharmacologyonline 2007;3:470-7.
21. Ghaisas MM, Bulani VD, Suralkar AA, Limaye RP. Effect of Calotropis gigantea on clonidine and haloperidol induced catalepsy. Pharmacologyonline 2009;3:484-8.
22. Bhargava KP, Singh N. Anti-stress activity of Ocimum sanctum Linn. Indian J Med Res 1981;73:443-51.
23. Jadhav JH, Balsara JJ, Chandorkar AG. Involvement of histaminergic mechanisms in the cataleptogenic effect of clonidine in mice. J Pharm Pharmacol 1983;35:671-3.
24. Lakdawala AD, Dadkar NK, Dohadwalla AN. Action of clonidine on the mast cells of rats. J Pharm Pharmacol 1980;32:790-1.
25. Brekhman LI, Dardymov IV. New substances of plant origin which increase nonspecific resistance. Annu Rev Pharmacol 1969;9:419-28.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.