STUDY OF THE ANXIOLYTIC ACTIVITY OF ETHANOLIC EXTRACT OF ROOT OF ACORUS CALAMUS IN ALBINO MICE
Keywords:Anxiolytic, Elevated plus maze, Diazepam, Acorus calamus
Objective: Anxiety is classified as a form of sub-acute or chronic fear. Root of Acorus calamus has been traditionally used as an anxiolytic. The aim of the study is to assess the anxiolytic activity of ethanolic extract of A. calamus (EEAC) by elevated plus-maze test on Albino mice.
Methods: Albino mice of either sex were taken and divided into five groups, each consisting of 5 mice. One group was used as control, one as standard (diazepam), and three as test groups treated with 100, 200, and 400 mg/kg of EEAC. The drugs, that is, 10 ml/kg of normal saline for control, diazepam 2 mg/kg (standard), and 100, 200, and 400 mg/kg EEAC (test groups) were injected intraperitoneally (i.p.), 30 min before placing them in the center of the maze. The preferences of the animal to open/enclosed arm, average time spent in open arm, and numbers of entries in open arm were compared in each group. Data were statistically analyzed by one-way analysis of variance followed by multiple Dunnett’s test.
Results: The number of entries in open arm and the average time spent in the open arm by the mice is increased by EEAC in a dose-dependent manner.
Conclusion: EEAC has anxiolytic activity.
Reus VI. Psychiatric disorders. Mental disorders. In: Kasper D, Braunwald E, Fauci A, Hauser SL, Longo DL, Jameson JL, editors. Harrison’s Principles of Internal Medicine. 17th ed. New York: The McGraw-Hill Companies, Inc.; 2008. p. 2717-23.
Baldessarini RJ. Drug therapy of depression and anxiety disorders. In: Brunton L, Parker K, editors. Goodman and Gilman’s the Pharmacological Basis of Therapeutics. 12th ed. New York: The McGraw-Hill Companies, Inc.; 2011:583-607.
Trivedi JK, Gupta PK. An overview of Indian research in anxiety disorders. Indian J Psychiatry 2010;52:210-8.
Elisendranth SJ, Lichtmacher JE. Psychiatric disorders. In: McPhee SJ, Papadakis MA, editors. Current Medical Diagnosis and Treatment. 47th ed. New York: The McGraw-Hill Companies, Inc.;2008. p. 807-902.
Kaur D, Kaur D, Bains N, Chopra A, Arora P. Anti-anxiety evaluation of extracts of stigma maydis (corn silk). Int J Pharm Pharm Sci 2015;8:309-12.
Vyawahare NS, Khandelwal AR, Batra VR, Nikam AP. Herbal Anticonvulsants. J Herb Med Toxicol 2007;1:9-14.
Elaya RA, Vijayalakshmi M, Devalarao G. Acorus calamus Linn, chemistry and biology. Res J Pharmacol 2009;2:256-61.
Meena AK, Rao MM, Singh A, Kumari S. Physiochemical and preliminary phytochemical studies on the rhizome of Acorus calamus Linn. Int J Pharm Pharm Sci 2010;2:130-1.
The Chemist and Druggist. Extracta liquida. In: Pharmaceutical Formulas PF. 11th ed., Vol. 1. London: The Chemist and Druggist; 1950. p. 183-6.
OECD Guideline (Organization for Economic Corporation and Development).OECD Guidelines for testing of chemicals (internet). France: OECD Publishing; 2006. p. 1-27. Available from: http://www. oecdbookshop.org/oecd/index. [Last accessed on 2011 Aug 02].
Ghosh MN. Toxicity studies. In: Fundamentals of Experimental pharmacology. 5th ed. Kolkata, India: Hilton and Company; 2008. p. 173-8.
Kaur S, Sharma A, Bedi PM. Evaluation of anxiolytic effect of Melilotus officinalis extracts in mice. Asian J Pharm Clin Res 2017;10:396-9.
Vogel HG. Psychotropic and neurotropic activity. In: Drug Discovery and Evaluation, Pharmacological Assays. 2nd ed. Berlin: Springer; 2002. p. 401-94.
Agarwal SS, Paridhavi M. Screening methods used for herbal drugs. In: Herbal Drug Technology. 1st ed. Hyderabad: Universities Press; 2007. p. 490-624.
Yende SR, Harle UN, Ittadwar AM. Anxiolytic activity of marine macroalgae Sargassum ilicifolium and Padina tetrastomatica in mice. Int J Pharm Pharm Sci 2016;8:97-101.
Montgomery KC. The relation between fear induced by novel and exploratory behavior. J Comp Psychol 1995;48:254-60.
Kulkarni SK. Experiments on intact preparations. Pharmacology of CNS. In: Hand book of Experimental Pharmacology. 3rd ed. New Delhi: Vallabh Prakashan; 2005. p. 131-9.
Krishna HN, Sangha RB, Mishra N, Pai MR. Antianxiety activity of NR-ANX-C, a polyherbal preparation in rats. Indian J Pharmacol 2006;38:330-5.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.