BREAST CANCER ACTIVITY OF ISOLATED COMPOUND FROM TINOSPORA CORDIFOLIA AERIAL
Keywords:Column chromatography, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Michigan Cancer Foundation-7 cell line, Breast cancer, Tinospora cordifolia
Objectives: The present study undertaken to explore antioxidant and the cell line study of isolated compound from ethanol extract from Tinospora cordifolia belongs to the family Menispermaceae.
Methods: The air dried powdered sample of aerial parts of T. cordifolia was extracted in a Soxhlet using five different solvents. Most active ethanol extracts were purified using silica gel column chromatography. Characterized the structure of the isolated compound using Fourier transform infrared spectrum, 1H nuclear magnetic resonance, and liquid chromatography–mass spectrometry studies. Antioxidant and anticancer activity of isolated compound was determined using 2,2-diphenyl-1-picrylhydrazyl and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: Magnoflorine was isolated from most active ethanol extract from aerial parts of T. cordifolia which shows maximum antioxidant activity 64% at the tested concentration 500 μg/ml. The percentage of cell viability varied from 53.3% at the minimum tested concentration 3.12 μg/ml to 1.9% at the maximum tested concentration 100 μg/ml.
Conclusion: The isolated characterized compounds would be useful to prepare plant-based pharmaceutical preparation to treat various diseases linked with human diseases.
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