SCREENING ANTIBACTERIAL ACTIVITY OF VIETNAMESE PLANT EXTRACTS AGAINST HUMAN PATHOGENIC BACTERIA

LONG HOANG NGO; THI HAI YEN NGUYEN; VU KHAC TRAN; VU VAN DOAN; MINH VAN NGUYEN; HIEU TRUNG BUI

doi:10.22159/ajpcr.2021.v14i6.39790

Authors

LONG HOANG NGO VK-Tech Center, Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City.
THI HAI YEN NGUYEN Faculty of Science, Dong Nai University, Bien Hoa City.
VU KHAC TRAN Department of Pharmaceutical Chemistry and Pesticides Technology, School of Chemical Engineering, Hanoi University of Science and Technology.
VU VAN DOAN Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
MINH VAN NGUYEN Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeollabuk-do, Korea.
HIEU TRUNG BUI VK-Tech Center, Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i6.39790

Keywords:

Screening, Antibacterial, Human pathogenic bacteria, Vietnamese plant extracts

Abstract

Objectives: Infectious diseases caused by bacteria are a leading cause of death worldwide. Hence, the objectives of the study are aimed to evaluate the antibacterial activity against five human pathogenic bacteria of methanolic extracts from 66 plants collected from Vietnam.

Methods: The broth microdilution method was used to determine the minimum inhibitory concentration (MIC) of methanol extracts of 66 plant species against five bacterial strains.

Results: In this study, all the plant extracts were active against at least one train with MIC values ranging from 24 to 2048 μg/mL. Twenty-five plant extracts were active against all three Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus). Of these, the extracts of Macaranga trichocarpa (Rchb. f. and Zoll.) Mull. Arg. (Euphorbiaceae), Calophyllum inophyllum L. (Clusiaceae) and Caryodaphnopsis baviensis (Lecomte) Airy Shaw (Lauraceae) exhibited the highest antibacterial activity (MIC =24–128 μg/mL), followed by extracts of Betula alnoides Buch.- Ham. e × . D. Don (Betulaceae), Acronychia pedunculata (L.) Miq. (Rutaceae), Croton alpinus A. Chev. ex Gagnep. (Euphorbiaceae) (MIC =64–256 μg/mL). Furthermore, the extract of Rhus chinensis Mill. (Anacardiaceae) and Annona reticulata L. (Annonaceae) exhibited potent antibacterial activity against the two Bacillus species (MIC =32–64 μg/mL).

Conclusion: Results of this study reveal that plant extracts from Vietnam have highly antibacterial activity against Gram-positive bacteria. These results suggest that Vietnamese plant extracts may be a rich source of antibacterial drugs.

Downloads

Download data is not yet available.

References

Fauci AS. Infectious diseases: Considerations for the 21st century. Clin Infect Dis 2001;32:675-85.

Noumedem JA, Mihasan M, Lacmata ST, Stefan M, Kuiate JR, Kuete V. Antibacterial activities of the methanol extracts of ten Cameroonian vegetables against Gram-negative multidrug-resistant bacteria. BMC Complement Altern Med 2013;13:26.

Kannathasan K, Senthilkumar A, Venkatesalu V. In vitro antibacterial potential of some Vitex species against human pathogenic bacteria. Asian Pac J Trop Med 2011;4:645-8.

Abreu AC, McBain AJ, Simões M. Plants as sources of new antimicrobials and resistance-modifying agents. Nat Prod Rep 2012;29:1007-21.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.

de Barros Machado T, Leal IC, Kuster RM, Amaral AC, Kokis V, de Silva MG, et al. Brazilian phytopharmaceuticals--evaluation against hospital bacteria. Phytother Res 2005;19:519-25.

Tajkarimi MM, Ibrahim SA, Cliver DO. Antimicrobial herb and spice compounds in food. Food Control 2010;21:1199-218.

Vu TT, Kim H, Tran VK, Le Dang Q, Nguyen HT, Kim H, et al. In vitro antibacterial activity of selected medicinal plants traditionally used in Vietnam against human pathogenic bacteria. BMC Complement Altern Med 2016;16:32.

Lesueur D, De Rocca Serra D, Bighelli A, Hoi TM, Thai TH, Casanova J. Composition and antimicrobial activity of the essential oil of Acronychia pedunculata (L.) Miq. from Vietnam. Nat Prod Res 2008;22:393-8.

Ghimire BK, Tamang JP, Yu CY, Jung SJ, Chung IM. Antioxidant, antimicrobial activity and inhibition of α-glucosidase activity by Betula alnoides Buch. bark extract and their relationship with polyphenolic compounds concentration. Immunopharmacol Immunotoxicol 2012;34:824-31.

Auranwiwat C, Trisuwan K, Saiai A, Pyne SG, Ritthiwigrom T. Antibacterial tetraoxygenated xanthones from the immature fruits of Garcinia cowa. Fitoterapia 2014;98:179-83.

Siridechakorn I, Phakhodee W, Ritthiwigrom T, Promgool T, Deachathai S, Cheenpracha S, et al. Antibacterial dihydrobenzopyran and xanthone derivatives from Garcinia cowa stem barks. Fitoterapia 2012;83:1430-4.

Negi PS, Jayaprakasha GK, Jena BS. Antibacterial activity of the extracts from the fruit rinds of Garcinia cowa and Garcinia pedunculata against food borne pathogens and spoilage bacteria. LWT Food Sci Technol 2008;41:1857-61.

Jamkhande PG, Wattamwar AS. Annona reticulata Linn. (Bullock’s heart): Plant profile, phytochemistry and pharmacological properties. J Tradit Complement Med 2015;5:144-52.

Jamkhande PG, Wattamwar AS, Kankudte AD, Tidke PS, Kalaskar MG. Assessment of Annona reticulata Linn. leaves fractions for in vitro antioxidative effect and antimicrobial potential against standard human pathogenic strains. Alex J Med 2016;52:19-25.

Ali SM, Perveen S, Rizwani GH, Ahmad VU. Screening for the antimicrobial properties of the leaves of Calophyllum inophyllum Linn. (Guttiferae). J Chem Soc Pak 1999;21:174-8.

Cechinel Filho V, Meyre-Silva C, Niero R. Chemical and pharmacological aspects of the genus Calophyllum. Chem Biodivers 2009;6:313-27.

Saravanan R, Dhachinamoorthi D, Senthilkumar K, Thamizhvanan K. Antimicrobial activity of various extracts from various parts of Calophyllum inophyllum L. J Appl Pharm Sci 2011;1:102-6.

Nguyen Hoang A, Ripperger H, Porzel A, Tran Van S, Adam G. Neolignans from Caryodaphnopsis baviensis. Phytochemistry 1997;46:569-71.

Magadula JJ. Phytochemistry and pharmacology of the genus Macaranga: A review. J Med Plant Res 2014;8:489-503.

Fareza MS, Syah YM, Mujahidin D, Juliawaty LD, Kurniasih I. Antibacterial flavanones and dihydrochalcones from Macaranga trichocarpa. Z Naturforsch C Biosci 2014;69:375-80.

Djakpo O, Yao W. Rhus chinensis and Galla Chinensis--folklore to modern evidence: Review. Phytother Res 2010;24:1739-47.