• RAMESH BS Department of Botany, St. Joseph’s College (Autonomous), Bengaluru, Karnataka, India.
  • LOKESH RAVI Department of Botany, St. Joseph’s College (Autonomous), Bengaluru, Karnataka, India.



Gas chromatography-mass spectrometry, Protein-ligand docking, Acarbose, Pseuderanthemum bicolor, 2,2-Dibromocholestanone, Pseduosarsasapogenin-5,20-Dien Methyl Ether, Methanol, Chloroform, Ethyl acetate, Phytochemicals


Objective: Aim of this study is to evaluate theanti-diabetic activity of Pseuderanthemum bicolor commonly called limang-sugat by inhibiting alpha-amylase protein.

Methods: Leaves of P. bicolor were extracted with methanol, chloroform, and ethyl acetate. The extracts were subjected for alpha-amylase inhibition assay and gas chromatography–mass spectrometry (GC–MS) analysis. Phytochemical compounds identified by GC-MS were subjected for protein-ligand docking study against alpha-amylase protein. Acarbose was used as a positive standard drug.

Results: The major bioactive compounds obtained from methanol, chloroform, and ethyl acetate extracts were 1,6;2,3-Dianhydro-4-Deoxy-Beta-D-Ribo-Hexopyranose, Pseduosarsasapogenin-5,20-Dien, methyl ether/Hexatriacontane, Di-N-decylsulfone/Octadecanal, and squalene, respectively. A total of 19 secondary metabolites were subjected for protein–ligand docking study against the alpha-amylase protein. The reference drug acarbose demonstrated binding energy of −7.8 Kcal/mol and formed 20 hydrogen bonds with the enzyme. Acarbose signified high polar interaction with the amylase enzyme. Among the 19 test ligands, “2,2-Dibromocholestanone” from ethyl acetate extract exemplified the highest binding energy of −9.3 Kcal/mol. The next highest remarkable inhibition was showed by “Pseduosarsasapogenin-5,20-Dien Methyl Ether” present in the methanol extract, with a binding energy of -9.3 Kcal/mol with the formation of 2 hydrogen bonds.

Conclusion: From the result, it could be concluded that the P. bicolor leaves contain various bioactive compounds which are considered as a good anti-diabetic drug.


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Routh JI. Fundamentals of Inorganic, Organic and Biological Chemistry. Philadelphia, PA: W.B. Saunders Company; 1955.

Poretsky L, editor. Principles of Diabetes Mellitus. Berlin, Germany: Springer Science and Business Media; 2010.

Lati MS, David MG, Kinuthia RN. The clinical significance of potential drug-drug interactions and their targets for minimization among hypertensive diabetic outpatients at a kenyan referral hospital. Int J Pharm Pharm Sci 2020;12:6-11.

Jacob R. Sickening sweet. Distillations 2015;1:12-5.

Narayan KV, Zhang P, Kanaya AM, Williams DE, Engelgau MM, Imperatore G, et al. Diabetes: The pandemic and potential solutions. In: Disease Control Priorities in Developing Countries. 2nd ed. United States: The International Bank for Reconstruction and Development, The World Bank; 2006. p. 591-601.

Sharma R, Amin H, Ruknuddin G, Prajapati PK. Efficacy of Ayurvedic remedies in Type 2 diabetes: A review through works done at Gujarat Ayurved university, Jamnagar. J Med Nutr Nutraceuticals 2015;4:63.

Sugat L. Philipine Medicinal Plants, Pseuderanthemum bicolor (Schrank) Radik. Ex Lindau. Available from: http://www.stuartxchange. org/Limang-sugat.html. [Last accessed on 2018 Nov 10].

Palakkal L, Hukuman NH, Mullappally J. Antioxidant activities and chemical composition of various crude extracts of Lepidagathis keralensis. J Appl Pharm Sci 2017;7:182-9.

Jung M, Park M, Chul HL, Kang Y, Seok-Kang E, Ki-Kim S. Antidiabetic agents from medicinal plants. Curr Med Chem 2006;13:1.

Mc Lafferty FW. Registry of Mass Spectral Data. 4th ed. New York: Wiley; 1986.

Sithara NV, Komathi S, Rajalakshmi G. Identification of bioactive compounds using different solvents through FTIR studies and GC-MS analysis. J Med Plants Stud 2017;5:192-4.

Otuokere IE, Amaku AJ, Igwe KK, Chinedum GC. Medicinal studies on the phytochemical constituents of Justicia carnea by GC-MS analysis. Am J Food Sci Health 2016;2:71-7.

Ravi L, Krishnan K. Benzoyloxy-ethyl-carbamic acid: A novel anticancerous secondary metabolite produced by Streptomyces globosus VITLGK011. Indian J Exp Biol 2017;55:411-20.

Tamil IG, Dineshkumar B, Nandhakumar M, Senthilkumar M, Mitra A. In vitro study on α-amylase inhibitory activity of an Indian medicinal plant, Phyllanthus amarus. Indian J Pharmacol 2010;42:280.

Ravi L. Procyanidin B2 of Cassia fistula a potent inhibitor of COVID19 protease: A molecular dynamic simulation analysis. Asian J Pharm 2020;14:274-81.

Arumugam A, Wong NK. Comparison study of antioxidant, antimicrobial and cytotoxic properties of secondary metabolite and protein extracts from Clinacanthus nutans. Int J Pharm Pharm Sci 2020;12:98-102.



How to Cite

BS, R., and L. RAVI. “IN VITRO AND IN SILICO ALPHA-AMYLASE INHIBITION POTENTIAL (ANTI-DIABETIC ACTIVITY) OF PSEUDERANTHEMUM BICOLOR (SIMS) RADIK”. Asian Journal of Pharmaceutical and Clinical Research, vol. 13, no. 12, Dec. 2020, pp. 157-61, doi:10.22159/ajpcr.2020.v13i12.39824.



Original Article(s)