• ZAHOOR AHMAD BHAT Department of Botany, Section of Plant Pathology and Mycology, University of Kashmir, Hazaratbal, Srinagar, India.
  • ABDUL HAMID WANI Department of Botany, Section of Plant Pathology and Mycology, University of Kashmir, Hazaratbal, Srinagar, India.
  • JOHN MOHD WAR Department of Botany, Section of Plant Pathology and Mycology, University of Kashmir, Hazaratbal, Srinagar, India.
  • MOHD YAQUB BHAT Department of Botany, Section of Plant Pathology and Mycology, University of Kashmir, Hazaratbal, Srinagar, India.


Ganoderma a white rot fungus has been used as a folk remedy for promoting health and longevity for centuries. The vast amount of study has been performed on the medicinal properties of Ganoderma in general and Ganoderma lucidum in particular. The bioactivities of the metabolites reported from G. lucidum are immense. The main bioactive metabolites of G. lucidum consist of mainly polysaccharides and triterpenoids. The major bioactive polysaccharides isolated from Ganoderma species are β (1→3), β (1→4), and β (1→6)-D glucans. With respect to the pure chemical and structural points of view, G. lucidum polysaccharides are mostly composed of β-glucans, heteropolysaccharides, and glycoptoteins. The major component of this sugar molecule is glucose together with xylose, mannose, galactose, and fructose in different conformations. Many of these bioactive polysaccharides have shown activities against the major diseases of our time and the list of effects shown is huge. Various important bioactivities, namely, antitumor, antioxidant, cytotoxic, immunomodulatory, antibacterial, anti-inflammation, neuroprotective, hepatoprotective, anti-HIV, and so on have been shown by these bioactive polysaccharides. The main purpose of this review is to report the most bioactive polysaccharides from G. lucidum and other species of Ganoderma and to report their potential health benefits.

Keywords: Ganoderma lucidum, β-glucans, Bioactive metabolites, Antitumor, Antioxidant


1. Chang ST, Miles PG. Mushrooms biology a new discipline. Mycologist 1992;6:64-5.
2. Hawksworth DL. The magnitude of fungal diversity. The 1.5 million species estimate revisited. Mycol Res 2001;105:1422-32.
3. Chang ST, Miles PG. Mushroom’s Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. Boca Raton, London: CRC Press; 2004.
4. Chang ST, Mshigeni KE. Mushroom and their Human Health: Their Growing Significance as Potent Dietary Supplements. Windhoek: The University of Namibia; 2001. p. 1-79.
5. Rahi DK, Malik D. Diversity of mushrooms and their metabolites of nutraceuticals and therapeutic significance. J Mycol 2016;2016:7654123.
6. Seo GS, Kirk PM. Ganodermataceae: Nomenclature and classification. In: Flood J, Bridge PD, Holderness P, editors. Ganoderma Disease of Perennial Crops. Wallingford, UK: CABI Publishing; 2000. p. 3-22.
7. Richter C, Wittstein K, Kirk PM, Stadler M. An assessment of the taxonomy and chemotaxonomy of Ganoderma. Fungal Divers 2015;71:1-15.
8. Jong SC, Birmingham JM. Medicinal benefits of the mushroom Ganoderma. Adv Appl Microbiol 1992;37:101-34.
9. Jonathan SG, Kigigha LT, Ohimain E. Evaluation of the inhibitory potentials of eight higher Nigerian fungi against pathogenic microorganisms. Afr J Biomed Res 2008;11:197-202.
10. Leung SW, Yeung KY, Ricky YL, Man YK. Lingzhi (Ganoderma) research: The past, present and future perspectives. In: Lin ZB, editor. Ganoderma: Genetics, Chemistry, Pharmacology and Therapeutics. Beijing: Beijing Medical University Press; 2002. p. 1-9.
11. Paterson RR. Ganoderma a therapeutic fungal biofactory. Phytochemistry 2006;67:1985-2001.
12. Ziegenbein FC, Hanssen HP, Konig WA. Secondary metabolites from Ganoderma lucidum and Spongiporus leucomallellus. Phytochemistry 2006;67:202-21.
13. Sanodiya BS, Thakur GS, Baghel RK, Prasad GB, Bisen PS. Ganoderma lucidum: A potent pharmacological macrofungus. Curr Pharm Biotechnol 2009;10:717-42.
14. Ríos JL, Andújar I, Recio MC, Giner RM. Lanostanoids from fungi: A group of potential anticancer compounds. J Nat Prod 2012;75:2016-44.
15. Rios JL, Andujar I. Lanostanoids from fungi as potential medicinal agents. In: Fungal Metabolites. Cham: Springer; 2017. p. 931-64.
16. Nishitoba T, Oda K, Sato H, Sakamura S. Novel triterpenoids from the fungus Ganoderma lucidum. Agri Biol Chem 1988;52:367-72.
17. Mizushina Y, Hanashima L, Yamaguchi T, Takemura M. A mushroom fruiting body inducing substance inhibits activities of replicative DNA polymerases. Biochem Biophys Res Commun 1988;249:17-22.
18. Wasser SP, Weis AL. Therapeutic effects of substance occurring in higher basidiomycete mushrooms: A modern perspective. Crit Rev Immunol 1999;19:65-96.
19. Wasser SP. Reishi or Ling Zhi (Ganoderma lucidum), Encyclopedia of Dietary Supplements. New York, USA: Marcel Dekker; 2005. p. 603-22.
20. Liew CW, Lee SS, Wang SY. The Effect of Ganoderma lucidum on induction of differentiation in leukemic U937-cells. Anticancer Res 1992;12:1211-6.
21. Adams M, Christen M, Plitzko I, Zimmermann S, Brun R, Kaiser M, et al. Antiplasmodial lanostanes from the Ganoderma lucidum mushroom. J Nat Prod 2010;73;897-900.
22. Cheng CR, Yue QX, Wu ZY, Song XY, Tao SJ, Wu XH, et al. Cytotoxic triterpenoids from Ganoderma lucidum. Phytochemistry 2010;71:1579-85.
23. Mizuno T, Saito H, Nishitoba T, Kawagashi H. Antitumor active substances from mushrooms. Food Rev Int 1995;11:23-61.
24. Wachtel-Galor S, Yuen J, Buswell JA, Benzie IFF. Ganoderma lucidum (Lingzhi or Reishi): A medicinal mushroom. In: Herbal Medicine: Bimolecular and Clinical Aspects. Boca Raton, USA: CRC Press Taylor and Francis; 2011.
25. Wang SY, Hsu ML, Hsu HC, Tzeng CH, Lee SS, Shiao MS, et al. The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T-lymphocytes. Int J Cancer 1997;70:699-705.
26. Yuen JW, Gohel MD. Anticancer effects of Ganoderma lucidum: A review of scientific evidence. Nutr Cancer 2005;53:11-7.
27. Yen GC, Wu JY. Antioxidant and radical scavenging properties of extracts from Ganoderma tsugae. Food Chem 1999;65:375-9.
28. Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 2002;60:258-74.
29. Kim DH, Shim SB, Kim NJ, Jang NJ. Beta glucuronidase-inhibitory activity and hepatoprotective effect of Ganoderma lucidum. Biol Pharm Bull 1999;22:162-4.
30. Hikino H, Konno C, Mirin Y, Hayashi T. Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies. Plant Med 1985;4:339-40.
31. Seto SW, Lam TY, Tam HL, Au AL, Chan SW, Wu JH, et al. Novel hypoglycemic effects of Ganoderma lucidum water-extract in obese/ diabetic (+db/+db) mice. Phytomedicine 2009;16:426-36.
32. Gao Y, Tang W, Gao H, Chan E, Lan J, Li X, et al. Antimicrobial activity of the medicinal mushroom Ganoderma. J Food Rev Int 2005;21:211-29.
33. Mizuno T, Wang G, Zhang J, Kawagishi H, Nishitoba T, Li J. Reishi, Ganoderma lucidum and Ganoderma tsugae: Bioactive substances and medicinal effects. Food Rev Int 1995;11:151-66.
34. Berger A, Rein D, Kratky E, Monnard I, Hajjaj H, Meirim I, et al. Cholesterol-lowering properties of Ganoderma lucidum in vitro, ex vivo, and in hamsters and minipigs. Lipids Health Dis 2004;3:2.
35. Stanley G, Harvey K, Slivova V, Jiang J. Ganoderma lucidum suppresses angiogenesis through the inhibition of secretion of VEGF and TGF-beta1 from prostate cancer cells. Biochem biophys res commun 2005;330:46-52.
36. Hsu SC, Ou CC, Chuang TC, Li JW, Lee YJ, Wang V, et al. Ganoderma tsugae extract inhibits expression of epidermal growth factor receptor and angiogenesis in human epidermoid carcinoma cells: In vitro and in vivo. Cancer Lett 2009;281:108-16.
37. Park EJ, Ko G, Kim J, Sohn DH. Anti-fibrotic effects of a polysaccharide extracted from Ganoderma lucidum, glycyrrhizin, and pentoxifylline in rats with cirrhosis induced by biliary obstruction. Biol Pharm Bull 1997;20:417-20.
38. El-Mekkawy S, Meselhy MR, Nakamura N, Tezuka Y, Hattori M, Kakiuchi N, et al. Anti-HIV-1 and anti-HIV-1- protease substances from Ganoderma lucidum. Phytochemistry 1988;49:1651-7.
39. Noguchi M, Kakuma T, Tomiyasu K, Kurita Y, Kukihara H, Konishi F, et al. Effect of an extract of Ganoderma lucidum in men with lower urinary tract symptoms: A double- blind, placebo-controlled randomized and dose-ranging study. Asian J Androl 2008;10:651-8.
40. Kawagishi H, Fukuhara F, Sazuka M, Kawashima A, Mitsubori T, Tomita T. 50-Deoxy-50-methylsulphinyladenosine, a platelet aggregation inhibitor from Ganoderma lucidum. Phytochemistry 1993;32:239-41.
41. Shiao MS, Lee Kuan R, Lin LJ, Wang CT. Natural products and biological activities of the Chinese medicinal fungus Ganoderma lucidum. In: Food Phytochemicals for Cancer Prevention II. Vol. 547. Springer; 1994. p. 342-54.
42. Wang YY, Khoo KH, Chen ST, Lin CC, Wong CH, Lin CH. Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: Functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities. Bioorg Med Chem 2002;10:1057-62.
43. Smania EFA, Monache FD, Smania A Jr., Yunes RA, Cuneo RS. Antifungal activity of sterols and triterpenes isolated from Ganoderma annulare. Fitoterapia 2003;74:375-7.
44. Sun J, He H, Xie BJ. Novel antioxidant peptides from fermented mushroom Ganoderma lucidum. J Agric Food Chem 2004;52:6646-52.
45. Akihisa T, Nakamura Y, Tagata M, Tokuda H, Yasukawa K, Uchiyama E, et al. Anti-inflammatory and anti-tumor-promoting effects of triterpene acids and sterols from the fungus Ganoderma lucidum. Chem Biodivers 2007;4:224-31.
46. Ferreira I, Vaz J, Vasconcelos MH, Martins A. Compounds from wild mushrooms with antitumor potential. Anticancer Agents Med Chem 2010;10:424-36.
47. Ooi VE, Liu F. A review of pharmacological activities of mushroom polysaccharides. Int J Med Mushrooms 1999;1:195-206.
48. Ooi VE, Liu F. Immunomodulation and anti-cancer activity of polysaccharide protein complexes. Curr Med Chem 2000;7:715-29.
49. Konno S, Aynehchi S, Dolin DJ, Schwartz AM, Choudhury MS, Tazaki H. Anticancer and hypoglycemic effects of polysaccharides in edible and medicinal Maitake mushroom (Grifola frondosa (Dicks.:Fr.) S.F.Gray). Int J Med Mushrooms 2002;4:185-95.
50. Ohno N, Harada T, Masuzawa S, Miura NN. Antitumor activity and hematopoietic response of a ?-glucan extracted from an edible and medicinal mushroom Sparassis crispa Wulf.:Fr. Int J Med Mushrooms 2002;4:13-26.
51. Cai X, Pi Y, Zhou X, Tian L, Qiao S, Lin J. Hepatoma cell growth inhibition by inducing apoptosis with polysaccharide isolated from Turkey tail medicinal mushroom, Trametes versicolor (L.: Fr.) Lloyd (Aphyllophoromycetideae). Int J Med Mushrooms 2010;12:257-63.
52. Ren L, Hemar Y, Perera CO, Buchanan PK. Antibacterial and antioxidant activities of aqueous extracts of eight edible mushrooms. Bioact Carbohydr Diet Fibre 2014;3:41-51.
53. Ferreira IC, Heleno SA, Reis FS, Stojkovic D, Queiroz MJ, Vasconcelos MH, et al. Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities. Phytochemistry 2015;114:38-55.
54. Chan WK, Law HK, Lin ZB, Lau YL, Chan GC. Response of human dendritic cells to different immunomodulatory polysaccharides derived from mushroom and barley. Int Immunol 2007;19, 891-9.
55. Chow-Chin T, Yew-Keong C, Mohamed S, Mustapha NM, Umar NA. Efficacy of G. lucidum on plasma lipids and lipoproteins in rats fed with high cholesterol diet. Nutr Food Sci 2008;38:229-38.
56. Lemieszek M, Rzeski W. Anticancer properties of polysaccharides isolated from fungi of the basidiomycetes class. Wspolczesna Onkol 2012;16:285-9.
57. Zhang W, Zhang Q, Deng W, Li Y, Xing G, Shi X, et al. Neuroprotective effect of pre-treatment with G. lucidum in cerebral ischemia/reperfusion injury in rat hippocampus. Neural Regen Res 2014;9:1446-52.
58. Jang SH, Cho SW, Yoon HM, Jang KJ, Song CH, Kim CH. Hepatoprotective evaluation of G. lucidum pharmacopuncture: In vivo studies of ethanol-induced acute liver injury. J Pharmacopuncture 2014;17:16-24.
59. Liu X, Yuan JP, Chung CK, Chen XJ. Antitumor activity of the sporoderm-broken germinating spores of G. lucidum. Cancer Lett 2002;182:155-61.
60. Sun LX, Lin ZB, Duan XS, Qi HH, Yang N, Li M, et al. Suppression of the production of transforming growth factor b1, interleukin-10, and vascular endothelial growth factor in the B16F10 cells by G. lucidum polysaccharides. J Interferon Cytokine Res 2014;34:667-75.
61. Choi YJ, Yang HS, Jo JH, Lee SC, Park TY, Choi BS, et al. Anti-amnesic effect of fermented G. lucidum water extracts by lactic acid bacteria on scopolamine-induced memory impairment in rats. Prev Nutr Food Sci 2015;20:126-32.
62. Wang SQ, Li XJ, Qiu HB, Jiang ZM, Simon M, Ma XR, et al. Anti-epileptic effect of G. lucidum Polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons. PLoS One 2014;9:e102161.
63. Chang CJ, Lin CS, Lu CC, Martel J, Ko YF, Ojcius DM, et al. G. lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun 2015;6:74-89.
64. Nithya M, Ambikapathy V, Panneerselvam A. Studies on antimicrobial potential of different strains of G. lucidum (Curt..: Fr.) P. Karst. Int J Pharm Sci Rev Res 2013;21:317-20.
65. Celik GY, Onbasli D, Altinsoy B, Alli H. In vitro antimicrobial and antioxidant properties of G. lucidum extracts grown in Turkey. Phys Med 2014;4:709-22.
66. Nayak RN, Dixitraj PT, Nayak A, Bhat K. Evaluation of antimicrobial activity of spore powder of G. lucidum on clinical isolates of Prevotella intermedia: A pilot study. Contemp Clin Dent 2015;6:248-52.
67. Pan D, Zhang D, Wu J, Chen C, Xu Z, Yang H, et al. Antidiabetic, antihyperlipidemic and antioxidant activities of a novel proteoglycan from G. lucidum fruiting bodies on db/db mice and the possible mechanism. PLoS One 2013;8:e68332.
68. Matsuzaki H, Shimizu Y, Iwata N, Kamiuchi S, Suzuki F, Iizuka H, et al. Antidepressant-like effects of a water-soluble extract from the culture medium of G. lucidum mycelia in rats. BMC Complement Altern Med 2013;13:1-8.
69. Li LF, Liu HB, Zhang QW, Li ZP, Wong TL, Fung HY, et al. Comprehensive comparison of polysaccharides from Ganoderma lucidum and G. sinense: Chemical, antitumor, immunomodulating and gut-microbiota modulatory properties. Sci Rep 2018;8:1-12.
70. Sone Y, Okuda R, Wada N, Kishida E, Misaki A. Structure and antitumor activities of polysaccharides isolated from fruiting body and the growing culture of mycelium of Ganoderma lucidum. Agric Biol Chem 1985;49:2641-53.
71. Miyazaki T, Nishijima M. Studies on fungal polysaccharides. XXVII. Structural examination of water-soluble, anti tumor polysaccharide of Ganoderma lucidum. Chem Pharm Bull 1981;29:3611-6.
72. Ukai S, Yokoyama S, Hara C, Kiho T. Structure of an alkali soluble polysaccharide from the fruit body of Ganoderma japonicum Lloyd. Carbohy Res 1982;105:237-45.
73. Gao B, Yang GZ. Immunoregulatory effect and antitumor, antiviral, antivirus activity of Ganoderma applanatum polysaccharide. Int J Immunopharmacol 1991;13:731.
74. Wang G, Zhang J, Mizuno T, Zhuang C. Antitumor active polysaccharides from the Chinese mushroom Songshan lingzhi, the fruiting body of Ganoderma tsugae. Biosci Biotechnol Biochem 1993;57:894-900.
75. Bao XF, Wang XS, Dong Q, Fang JN, Li XY. Structural features of immunologically active polysaccharides from Ganoderma lucidum. Phytochemistry 2002;59:175-81.
76. Ye LB, Zhang JS, Yang Y, Zhou S, Liu Y, Tang Q, et al. Structural characterisation of a heteropolysaccharide by NMR spectra. Food Chem 2009;112:962-6.
77. Pan Y, Hao Y, Chu TW, Li CQ. Ultrasonic-assisted extraction, chemical characterization of polysaccharides fromYunzhi mushroom and its effect on osteoblast cells. Carbohydr Polym 2010;80:922-6.
78. Kozarski M, Klaus A, Niksic M, Vrvic MM, Todorovic N, Jakovljevic D, et al. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. J Food Composit Anal 2012;26:144-53.
79. Zhang H, Li WJ, Nie SP, Chen Y, Wang YX, Xie MY. Structural characterisation of a novel bioactive polysaccharide from Ganoderma atrum. Carbohydr Polym 2012;88:1047-54.
80. Peng Y, Zhang L, Zeng F, Kennedy JF. Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium. Carbohydr Polym 2005;59:385-92.
81. Bao XF, Liu CP, Fang JN, Li XY. Structural and immunological studies of a major polysaccharide from spores of Ganoderma lucidum (Fr.) Karst. Carbohydr Res 2001;332:67-74.
82. Duggan C, Gannon J, Walker WA. Protective nutrients and functional foods for the gastrointestinal tracts. Am J Clin Nutr 2002;75:789-808.
83. Benkeblia N. Polysaccharides: Natural Fibers in Food and Nutrition. London, Boca Raton, (FL): CRC Press, Taylor and Francis Group; 2014.
84. Giavasis I. Bioactive fungal polysaccharides as potential functional ingredients in food and nutraceuticals. Curr Opin Biotechnol 2014;26:162-73.
85. Wasser SP. Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Appl Microbiol Biotechnol 2011;89:1323-32.
86. Chang ST, Wasser SP. The role of culinary-medicinal mushrooms on human welfare with a pyramid model for human health. Int J Med Mushrooms 2012;14:95-134.
87. Mizuno M, Nishitani Y. Immunomodulating compounds in basidiomycetes. J Clin Biochem Nutr 2013;52:202-7.
88. Shaoping N, Zhang H, Li W, Xie M. Current development of polysaccharides from Ganoderma: Isolation, structure and bioactivities. Bioact Carbohydr Diet Fibre 2013;1:10-20.
89. Lin YL, Liang YC, Lee SS, Chiang BL. Polysaccharide purified from Ganoderma lucidum induced activation and maturation of human monocyte-derived dendritic cells by the NF-?B and p38 mitogen activated protein kinase pathways. J Leukoc Biol 2005;78:533-43.
90. Zhang M, Cui SW, Cheung PC, Wang Q. Antitumor polysaccharides from mushrooms: A review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Technol 2007;18:4-19.
91. Chen Y, Xie MY, Nie SP, Li C, Wang YX. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem 2008;107:231-41.
92. Wang JG, Zhang LN. Structure and chain conformation of five water soluble derivatives of a b-D-glucan isolated from Ganoderma lucidum. Carbohydr Res 2009;344:105-12.
93. Nie S, Zhang H, Li W, Xie M. Current development of polysaccharides from Ganoderma: Isolation, structure and bioactivities. Bioact Carbohydr Diet Fibre 2013;1:10-20.
94. Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA. Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). Int Immunopharmacol 2007;7:701-24.
95. Jia J, Zhang X, Hu YS, Wu Y, Wang QZ, Li NN, et al. Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ diabetic rats. Food Chem 2009;115:32-6.
96. Zhang J, Tang Q, Zimmerman-Kordmann M, Reutter W, Fan H. Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum. Life Sci 2002;71:623-38.
97. Zong A, Cao H, Wang F. Anticancer polysaccharides from natural resources: A review of recent research. Carbohydr Polym 2012;90:1395-410.
98. Gao YH, Gao H, Chan E, Tang WB, Xu A, Yang H, et al. Antitumor activity and underlying mechanisms of ganopoly, the refined polysaccharides extracted from Ganoderma lucidum, in mice. Immunol Invest 2005;34:171-98.
99. Liu YJ, Shena J, Xia YM, Zhang J. The polysaccharides from Ganoderma lucidum: Are they always inhibitors on human hepatocarcinoma cells? Carbohydr Polym 2012;90:1210-5.
100. Zhao L, Dong Y, Chen G, Hu Q. Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum. Carbohydr Polym 2010;80:783-9.
101. Kim BK, Cho HY, Kim JS, Kim HW, Choi EC. Studies on constituents of higher fungi of Korea (LXVIII). Antitumor components of the cultured mycelia of Ganoderma lucidum. Korean J Pharmacol 1993;24:203-12.
102. Joseph S, Sabulal B, George V, Antony K, Janardhanan K. Antitumor and anti-inflammatory activities of polysaccharidesisolatedfrom Ganoderma lucidum. Acta Pharm 2011;61:335-42.
103. Cao QZ, Lin ZB. Antitumor and anti-angiogenic activity of Ganoderma lucidum polysaccharides peptide. Acta Pharmacol Sin 2004;25:833-8.
104. Lowe E, Rice P, Ha T. A(1-->3)-b-D-linked heptasaccharide is the unitligand for glucan pattern recognition receptors on human monocytes. Microbes Infect 2001;3:789-97.
105. Yan J, Vetvicka V, Xia Y, Coxon A, Carrol MC, Mayadas TN, et al. Beta-glucan, a ‘‘specific’’ biologic response modifier that uses antibodies to target tumors for cytotoxic recognition by leukocyte complement receptor Type 3 (CD11b/CD18). J Immunol 1999;163:3045-52.
106. Ooi LS, Ooi VEC, Fung MC. Induction of gene expression of immunomodulatory cytokines in the mouse by a polysaccharide from Ganoderma lucidum (Curt.: Fr.) P. Karst. (Aphyllophoromycetideae). Int J Med Mushrooms 2002;4:27-35.
107. Pan K, Jiang Q, Liu G, Miao X, Zhong D. Optimization extraction of Ganoderma lucidum polysaccharides and its immunity and antioxidant activities. Int J Biol Macromol 2013;55:301-6.
108. Zhang J, Liu YJ, Park HS, Xia YM. Antitumor activity of sulfated extracellular polysaccharides of Ganoderma lucidum from the submerged fermentation broth. Carbohydr Polym 2012;87:1539-44.
109. Ma CW, Feng M, Zhai X, Hu M. Optimization for the extraction of polysaccharides from Ganoderma lucidum and their antioxidant and antiproliferative activities. J Taiwan Inst Chem Eng 2013;44:886-94.
110. Cao QZ, Lin ZB. Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell. Life Sci 2006;78:1457-63.
111. Kumar DS, Senthilkumar P, Surendran L, Sudhagar B. Ganoderma lucidum oriental mushroom mediated synthesis of gold nanoparticles conjugated with doxorubicin and evaluation of its anticancer potential on human breast cancer MCF-7/DOX cells. Int J Pharm Pharm Sci 2017;9:267-74.
112. Liu W, Wang HY, Pang XB, Yao WB. Characterization and antioxidant activity of two low- molecular-weight polysaccharides purified from the fruiting bodies of Ganoderma lucidum. Int J Biol Macromol 2010;46:451-7.
113. Kozarski M, Klaus A, Niksic M, Jakovljevic S, Helsper JP, Van Griensven LJ. Antioxidative and immunomodulating activities of polysaccharide extract of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. Food Chem 2011;129:1667-75.
114. Ping CX, Yan C, Shuibing L, YouGou C. Free radical scavenging of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes and immunity activities in cervical carcinoma rats. Carbohydr Polym 2009;77:389-93.
115. Mau JL, Tsai SY, Tseng YH, Huang SJ. Antioxidant properties of hot water extracts from Ganoderma tsugae Murrill. LWT Food Sci Technol 2005;38:589-97.
116. Mau JL, Tsai SY, Tseng YH, Huang SJ. Antioxidant properties of methanolic extracts from Ganoderma tsugae. Food Chem 2005;93:641-9.
117. Tseng YH, Yang JH, Mau JL. Antioxidant properties of polysaccharides from Ganoderma tsugae. Food Chem 2008;107:732-8/
118. Shi M, Zhang Z, Yang Y. Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide. Carbohydr Polym 2013;95:200-6.
119. Kao PF, Wang SH, Hung WT, Liao YH, Lin CM, Yang WB. Structural characterization and antioxidative activity of low-molecular-weights Beta-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies. J Biomed Biotechnol 2012;2012:1-8.
120. Kan Y, Chen T, Wu Y, Wu J. Antioxidant activity of polysaccharide extracted from Ganoderma lucidum using response surface methodology. Int J Biol Macromol 2015;72:151-7.
121. Zhonghui Z, Xiaowei Z, Fang F. Ganoderma lucidum polysaccharides supplementation attenuates exercise-induced oxidative stress in skeletal muscle of mice. Saudi J Biol Sci 2014;21:119-23.
122. Li XL, Zhou AG, Li XM. Inhibition of Lycium barbarium polysaccharides and Ganoderma lucidum polysaccharides against oxidative injury induced by ?- irradiation in rat liver mitochondria. Carbohydr Polym 2007;69:172-8.
123. Chen TQ, Wu JZ. Efficient extraction technology of antioxidant crude polysaccharides from Ganoderma lucidum (Linghzi), ultrasonic-circulating extraction integrating with superfine-pulverisation. J Taiwan Inst Cheml Eng 2014;45:57-62.
124. Gao Y, Zhou S, Jiang W, Huang M, Dai X. Effects of Ganopoly (a Ganoderma lucidum polysaccharide extract) on the immune functions in advanced-stage cancer patients. Immunol Invest 2003;32:201-15.
125. Bach JP, Deuster O, Balzer-Geldsetzer M, Meyer B, Dodel R, Bacher M. The role of macrophage inhibitory factor in tumorigenesis and central nervous system tumors. Cancer 2009;115:2031-40.
126. Mantovani A, Sica A. Macrophages, innate immunity and cancer: Balance, tolerance, and diversity. Curr Opin Immunol 2010;22:231-7.
127. You YH, Lin ZB. Protective effects of Ganoderma lucidum polysaccharides peptide on injury of macrophages induced by reactive oxygen species. Acta Pharmacol Sin 2002;23:789-91.
128. Zhu XL, Chen AF, Lin ZB. Ganoderma lucidum polysaccharides enhance the function of immunological effect or cells in immune-suppressed mice. J Ethnopharmacol 2007;111:219-26.
129. Sun LX, Lin ZB, Li XJ, Li M, Lu J, Duan XS, et al. Promoting effects of Ganoderma lucidum polysaccharides on B16F10 cells to activate lymphocytes. Basic Clin Pharmacol Toxicol 2011;108:149-54.
130. Zhang QH, Lin ZB. The antitumor activity of Ganoderma lucidum (Curt Fr) P Karst (Ling Zhi) (Aphyllophoromycetideae) polysaccharides sis related to tumor necrosis factor-a and interferon-g. Int J Med Mushrooms 1999;1:207-15.
131. Cao LZ, Lin ZB. Regulation on maturation and function of dendritic cells by Ganoderma lucidum polysaccharides. Immunol Lett 2002;83:163-9.
132. Gao YH, Tang WB, Dai XH, Gao H, Chen G, Ye J, et al. Effects of water-soluble Ganoderma lucidum polysaccharides on the immune functions of patients with advanced lung cancer. J Med Food 2005;8:159-68.
133. Huang SQ, Ning ZX. Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity. Int J Biol Macromol 2010;47: 336-41.
134. Miles PG, Chang ST. Mushroom Biology: Concise Basics and Current Developments. Singapore: World Scientific; 1997. p. 194.
135. Gao Y, Zhou S, Huang M, Xu A. Antibacterial and antiviral value of the genus Ganoderma P. Karst species (Aphyllophoromycetideae): A review. Int J Med Mushrooms 2003;5:235-46.
136. Bhattacharyya C, De S, Basak A, Banerjee M, Snigdha M, Samajpati N. Antimicrobial activities of some basidiomycetous fungi. J Mycopathol Res 2006;44:129-35.
137. Wang JG, Ma ZC, Zhang LN, Fang YP, Jiang F, Phillips GO. Structure and chain conformation of water-soluble heteropolysaccharides from Ganoderma lucidum. Carbohydr Polym 2011;86:844-51.
138. Sullivan R, Smith JE Rowan NJ. Medicinal mushrooms and cancer therapy. Translating a traditional practice into Western medicine. Perspect Biol Med 2006;49:159-70.
139. Bai D, Chang NT, Li DH, Liu JX, You XY. Antiblastic activity of Ganoderma lucidum polysaccharides. Acta Agric Boreali Sin 2008;23:282-5.
140. Yoon SY, Eo SK, Kim YS, Lee CK, Han SS. Antimicrobial activity of Ganoderma lucidum extract alone and in combination with some antibiotics. Arch Pharm Res 1994;17:438-42.
141. Quereshi S, Pandey AK, Sandhu SS. Evaluation of antibacterial activity of different Ganoderma lucidum extracts. J Sci Res 2010;3:9-13.
142. Sheena N, Ajith TA, Mathew AT, Janardhanan KK. Antibacterial activity of three macrofungi, Ganoderma lucidum, Navesporus floccosa and Phellinus rimosus occurring in South India. Pharm Biol 2003;41:564-7.
143. Paul S, Sasikumar CS, Singh AR. Fabrication of silver nanoparticles synthesized from Ganoderma lucidum into the cotton fabric and its antimicrobial property. Int J Pharm Pharm Sci 2015;7:53-56.
144. Bhardwaj A, Gupta P, Kumar N, Mishra J, Kumar A, Misra K. Lingzhi or reishi mushroom, Ganoderma lucidum (Agaricomycetes), inhibits Candida biofilms: A metabolic approach. Int J Med Mushrooms 2017;19:685-96.
145. Cheung WM, Hui WS, Chu PW, Chiu SW, Ip NY. Ganoderma extract activates MAP kinases and induces the neuronal differentiation of rat pheochromocytoma PC12 cells. FEBS Lett 2000;486:291-6.
146. Zhao HB, Lin SQ, Liu JH, Lin ZB. Polysaccharide extract isolated from Ganoderma lucidum protects rat cerebral cortical neurons from hypoxia/reoxygenation injury. J Pharmacol Sci 2004;95:294-8.
147. Zhang J, Tang Q, Zhou C, Jia W, Da Silva L, Nguyen LD, et al. GLIS, a bioactive proteoglycan fraction from Ganoderma lucidum, displays antitumour activity by increasing both humoral and cellular immune response. Life Sci 2010;87:628-37.
148. Lai CS, Yu MS, Yuen WH, So KF, Zee SY, Chang RC. Antagonizing ?-amyloid peptide neurotoxicity of the anti-aging fungus Ganoderma lucidum. Brain Res 2008;1190:215-24.
149. Zhou Y, Qu ZQ, Zeng YS, Lin YK. Neuroprotective effect of preadministration with Ganoderma lucidum spore on rat hippocampus. Exp Toxicol Pathol 2012;64:673-80.
150. Selkoe DJ. Alzheimer’s disease: Genotypes, phenotypes, and treatments. Science 1997;275:630-1.
151. Heneka MT, O’Banion MK. Inflammatory processes in Alzheimer’s disease. J Neuroimmunol 2007;184:69-91.
152. Perry VH, Nicoll JA, Holmes C. Microglia in neurodegenerative disease. Nat Rev Neurol 2010;6:193-201.
153. Brown GC, Neher JJ. Microglial phagocytosis of live neurons. Nat Rev Neurosci 2014;15:209-16.
154. Fu R, Shen Q, Xu P, Luo JJ, Tang Y. Phagocytosis of microglia in the central nervous system diseases. Mol Neurobiol 2014;49:1422-34.
155. Gerard C, Rollins BJ. Chemokines and disease. Nat Immunol 2001;2:108-15.
156. Nagata T, Nagano I, Shiote M, Narai H, Murakami T, Hayashi T, et al. Elevation of MCP-1 and MCP-1/VEGF ratio in cerebrospinal fluid of amyotrophic lateral sclerosis patients. Neurol Res 2007;29:772-6.
157. Gao L, Tang H, Nie K, Wang L, Zhao J, Gan R, et al. MCP-1 and CCR2 gene polymorphisms in Parkinson’s disease in a Han Chinese cohort. Neurol Sci 2015;36:571-6.
158. El Khoury JB, Moore KJ, Means TK, Leung J, Terada K, Toft M, et al. CD36 mediates the innate host response to beta-amyloid. J Exp Med 2003;197:1657-66.
159. Hickman SE, El Khoury J. Mechanisms of mononuclear phagocyte recruitment in Alzheimer’s disease. CNS Neuro Disord Drug Targets 2010;9:168-73.
160. Selenica ML, Alvarez JA, Nash KR, Lee DC, Cao C, Lin X, et al. Diverse activation of microglia by chemokine (C-C motif) ligand 2 overexpression in brain. J Neuroinflammation 2013;10:856.
161. Ishizuka K, Kimura T, Igata-yi R, Katsuragi S, Takamatsu J, Miyakawa T. Identification of monocyte chemoattractant protein-1 in senile plaques and reactive microglia of Alzheimer’s disease. Psychiatry Clin Neurosci 1997;51:135-8.
162. Cai Q, Li Y, Pei G. Polysaccharides from Ganoderma lucidum attenuate microglia mediated neuroinflammation and modulate microglial phagocytosis and behavioural response. J Neuroinflammation 2017;14:63.
163. Huang S, Mao J, Ding K, Zhou Y, Zeng X, Yang W, et al. Polysaccharides from Ganoderma lucidum promotes cognitive function and neural progenitor proliferation in mouse model of Alzheimer’s disease. Stem Cell Rep 2017;8:84-94.
164. He CY, Li WD, Guo SX, Lin SQ, Lin ZB. Effect of polysaccharides from Ganoderma lucidum on streptozotocin- induced diabetic nephropathy in mice. J Asian Nat Prod Res 2006;8:705-11.
165. Meng G, Zhlu H, Yang S, Wu F, Zheng H, Chen E, et al. Attenuating effects of Ganoderma lucidum polysaccharides on myocardial collagen cross-linking relates to advanced glycation end product and antioxidant tenzymes in high-fat-diet and streptozotocin-induced diabetic rats. Carbohydr Polym 2011;84:180-5.
166. Gao YH, Lan J, Dai XH, Ye JX, et al. Aphase I/II study of Lingzhi mushroom Ganoderma lucidum (W.Curt:Fr.) Lloyd (Aphyllophoromycetideae) extract in patients with Type II diabetes mellitus. Int J Med Mushrooms 2004;6:33-9.
167. Zhang HN, He JH, Yuan L, Lin ZB. In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage. Life Sci 2003;73:2307-19.
168. Pan D, Wang L, Chen C, Teng B. Structure characterization of a novel neutral polysaccharide isolated from Ganoderma lucidum fruiting bodies. Food Chem 2012;135:1097-103.
169. Zhang GL, Wang YH, Ni W, Teng H, Lin ZB. Hepatoprotective role of Ganoderma lucidum polysaccharide against BCG-induced immune liver injury in mice. World J Gastroenterol 2002;15:728-33.
170. Gao Y, Huang M, Lin ZB, Zhou S. Hepatoprotective activity and the mechanisms of action of Ganoderma lucidum (Curt.:Fr.) P. Karst. (Ling Zhi, Reishi Mushroom). Int J Med Mushrooms 2003;5:27-35.
171. Pham HN, Hoang LS, Phung VT, Hsu TC. Hepatoprotective activity of Ganoderma lucidium (Curtis) P. Karst against cyclophosphamide-induced liver injury in mice. Cogent Biol 2016;2:1267421.
172. Zhu KX, Nie SP, Tan LH, Li C, Gong DM, Xie MY. Polysaccharide from Ganoderma atrum improves liver function in Type 2 diabetic rats via antioxidant action and short-chain fatty acids excretion. J Agric Food Chem 2016;64:1938-44.
173. Yu Y, Qian L, Du N, Liu Y, Zhao X. Ganoderma lucidum polysaccharide enhances radiosensitivity of hepatocellular carcinoma cell line HepG2 through Akt signaling pathway. Exp Ther Med 2017;14:5903-7.
174. Zhao GM, Zhang LX, Yu TM, Liu SM, Zhang LP. Chemical structure of water soluble polysaccharide from spores of Ganoderma lucidum. J Clin Pharmacol 2005;41:902-04.
175. Chen JH, Zhou JP, Zhang LN. Chemical structure of the water-insoluble polysaccharide isolated from the fruiting body of Ganoderma lucidum. Polym J 1998;30:838-42.
176. Huang SQ, Li JW, Li YQ, Wang Z. Purification and structural characterization of water-soluble neutral polysaccharide GLP-F1- 1from Ganoderma lucidum. Int J Biol Macromol 2011;48:165-9.
177. Zhang LX, Zhao GM, Wu MJ, Cao PY. Purification and characterization of water soluble polysaccharide GLPS 3 from sporderm-unbroken germinting spores of Ganoderma lucidum. J Clin Pharm 2006;41:230-2.
178. Dong Q, Wang Y, Shi L, Yao J, Li J, Ma F, et al. A novel water-soluble ?-D-glucan isolated from the spores of Ganoderma lucidum. Carbohydr Res 2012;353:100-5.
179. Amaral AE, Carbonero ER, Simao CG, Kadowaki MK, Sassaki GL, Osaku CA, et al. An unusual water-soluble b- glucan from the basidiocarp of the fungus Ganoderma resinaceum. Carbohydr Polym 2008;72:473-8.
180. Skalicka-Wozniak K, Szypowski J, ?os R, Siwulski M, Sobieralski K, Glowniak K, et al. Evaluation of polysaccharides content in fruit bodies and their antimicrobial activity of four Ganoderma lucidum (W Curt.: Fr.) P. Karst strains cultivated on different wood type substrates. Acta Soc Bot Pol 2012;81:17-21.
181. Wang CL, Pi CC, Kuo CW, Zhuang YJ, Khoo KH, Liu WH, et al. Polysaccharides purified from the submerged culture of Ganoderma formosanum stimulate macrophage activation and protect mice against Listeria monocytogenes infection. Biotechnol Lett 2011;33:2271-8.
182. YouGuo C, Zongji S, XiaoPing C. Modulatory effect of Ganoderma lucidum polysaccharides on serum antioxidant enzymes activities in ovarian cancer rats. Carbohydr Polym 2009;78:258-62.
183. Ye LB, Li JR, Zhang JS, Pan YJ. NMR characterization for polysaccharide moiety of a glycopeptide. Fitoterapia 2010;81:93-6.
184. Peng YF, Zhang LN, Zeng FB, Xu YX. Structure and antitumor activity of extracellular polysaccharides from mycelium. Carbohydr Polym 2003;54:297-303.
185. Li YQ, Fang L, Zhang KC. Structure and bioactivities of a galactoseric extracellular polysaccharide from submergedly cultured Ganoderma lucidum. Carbohydr Polym 2007;68:323-8.
186. Ye LB, Zhang JS, Ye XJ, Tang QJ, Liu Y, Gong C, et al. Structural elucidation of the polysaccharide moiety of a glycopeptides (GLPCW-II) from Ganoderma lucidum fruiting bodies. Carbohydr Res 2008;343:746-52.
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How to Cite
AHMAD BHAT, Z., A. HAMID WANI, J. M. WAR, and M. Y. BHAT. “MAJOR BIOACTIVE PROPERTIES OF GANODERMA POLYSACCHARIDES: A REVIEW”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 3, Mar. 2021, pp. 11-24, doi:10.22159/ajpcr.2021.v14i3.40390.
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