ANTIFUNGAL ACTIVITY OF ACTINOBACTERIA WITH A POTENTIAL TO INHIBIT RICE BLAST FUNGUS MAGNAPORTHE ORYZAE (ANAMORPH PYRICULARIA ORYZAE)
Keywords:Secondary metabolites, Streptomyces violaceoruber, Rice blast disease, Magnaporthe oryzae, Bioautography
Objective: The aims of the present study were to screen the actinobacteria with high potential ability to produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, Magnaporthe oryzae. Production of secondary metabolites was analysis by thin-layer chromatography and bioautography assay.
Methods: Screening and selection of potential Streptomyces sp. morphological, cultural, physiological, and biochemical characterization of the screened isolate was carried out. Antifungal compound was confirmed by bioautography assay.
Results: Bioautography method use in this study was found to be antifungal fraction from the crude extract. Antifungal secondary metabolites can be readily located on the plates by observing clear zones where active compounds inhibit fungal growth.
Conclusion: The bioautography assay shows that this isolates can produce antifungal compound. Therefore, this isolate proves to be a promising microbe which can be further studied for its applications a biocontrol agent against rice blast fungi.
Smith RM, Peterson WH, McCoy E. Oligomycin, a new antifungal antibiotic. Antibiot Chemother 1954;4:962-70.
Kim BS, Moon SS, Hwang BK. Isolation, identification, and antifungal activity of a macrolide antibiotic, oligomycin A, produced by Streptomyces libani. Can J Bot 1999;77:850-8.
Yang PW, Li MG, Zhao JY, Zhu MZ, Shang H, Li JR, et al. Oligomycins A and C, major secondary metabolites isolated from the newly isolated strain Streptomyces diastaticus. Folia Microbiol 2010;55:10-6.
Robertson J, Stevens K. Pyrrolizidine alkaloids. Nat Prod Rep 2014;31:1721-88.
Awla HK, Kadir J, Othman R, Rashid TS, Wong MY. Bioactive compounds produced by Streptomyces sp. isolate UPMRS4 and antifungal activity against Pyricularia oryzae. Am J Plant Sci 2016;7:1077-85.
Zhou L, Zhang X, Li C, Christensen MJ, Nan Z. Antifungal activity and phytochemical investigation of the asexual endophyte of Epichloë sp. from Festuca sinensis. Sci China Life Sci 2015;58:821.
Mishra J, Tewari S, Singh S, Arora NK. Biopesticides: Where we stand. In: Plant Microbes Symbiosis: Applied Facets. Vol. 37. 2015. p. 75.
Tyc O, Song C, Dickschat JS, Vos M, Garbeva P. The ecological role of volatile and soluble secondary metabolites produced by soil bacteria. Trends Microbiol 2017;25:280-92.
Adegboye MF, Babalola OO. Taxonomy and ecology of antibiotic producing actinomycetes. Afr J Agric Res 2012;7:2255-61.
Jog R, Nareshkumar G, Rajkumar S. Enhancing soil health and plant growth promotion by actinomycetes. In: Plant Growth Promoting Actinobacteria. Berlin, Germany: Springer 2016. p. 33-45.
El Tarabily KA, Nassar AH, Sivasithamparam K. Promotion of growth of bean (Phaseolus vulgaris L.) in a calcareous soil by a phosphate solubilizing, rhizosphere-competent isolate of Micromonospora endolithica. Appl Soil Ecol 2008;39:161-71.
El-Tarabily KA, Sivasithamparam K. Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Soil Biol Biochem 2006;38:1505-20.
Compant S, Duffy B, Nowak J, Clément C, Barka EA. Use of plant growth-promoting bacteria for biocontrol of plant diseases: Principles, mechanisms of action, and future prospects. Appl Environ Microbiol 2005;71:4951-9.
Mavrodi DV, Peever TL, Mavrodi OV, Parejko JA, Raaijmakers JM, Lemanceau P, et al. Diversity and evolution of the phenazine biosynthesis pathway. Appl Environ Microbiol 2010;76:866-79.
Chin-A-Woeng TF, Thomas-Oate JE, Lugtenberg BJ, Bloemberg GV. Introduction of the phzH gene of Pseudomonas chlororaphis PCL1391 extends the range of biocontrol ability of phenazine-1-carboxylic acid-producing Pseudomonas spp. strains. Mol Plant Microb Interact 2001;14:1006-15.
Bharti A, Kumar V, Gusain O, Bisht GS. Antifungal activity of actinomycetes isolated from Garhwal region. J Sci Eng Tech Mgt 2010;2:3-5.
Soares AC, Sousa CD, Garrido MD, Perez JO, Almeida NS. Soil streptomycetes with in vitro activity against the yam pathogens Curvularia eragrostides and Colletotrichum gloeosporioides. Braz J Microbiol 2006;37:456-61.
Jain PK, Jain PC. Isolation, characterization and antifungal activity of Streptomyces sampsonii GS 1322. Indian J Exp Biol 2007;45:203-6.
Chaudhary HS, Yadav J, Shrivastava AR, Singh S, Singh AK, Gopalan N. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). J Adv Pharm Technol Res 2013;4:118.
Riungu GM, Muthomi JW, Narla RD, Wagacha JM, Gathumbi JK. Management of Fusarium head blight of wheat and deoxynivalenol accumulation using antagonistic microorganisms. Plant Pathol 2008;7:13-9.
Bhavya T, Padma S. Antifungal activity of Cymbopogon citratus leaves extract against herbal drug contaminants. World J Pharm Pharm Sci 2014;3:768-75.
Srinivasan V, Mukkayyan N, Ajitkumar P. Highly deviated asymmetric division in very low proportion of mycobacterial mid-log phase cells. Open Microbiol J 2014;8:40.
Shirling ET, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Evol Microbiol 1966;16:313-40.
Isik K, Chun J, Hah YC, Goodfellow M. Nocardia salmonicida nom. rev., a fish pathogen. Int J Syst Evol Microbiol 1999;49:833-37.
Ellaiah P, Adinarayana G, Saisha V, Vasu P. An oligoglycosidic antibiotic from a newly isolated Streptomyces albovinaceus. Indian J Microbiol 2005;45:33.
Sambrook HC. Molecular Cloning: A Laboratory Manual. Philadelphia, PA: Williams and Wilkins Co., 1961.
Edgar RC. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004;32:1792-7.
Suleiman MM, McGaw LI, Naidoo V, Eloff J. Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species. Afr J Tradit Complement Altern Med 2010;7:64-78.
Waksman SA. The Actinomycetes. Vol. II. Classification, Identification and Descriptions of Genera and Species. London: Baillière, Tindall & Cox, Ltd., 7 & 8, Henrietta Street, W.C.2.1961. pp.ix+363pp.
Devanna NB, Vijayan J, Sharma TR. The blast resistance gene Pi54 of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains. PLoS One 2014;9:e104840.
Piotti E, Rigano MM, Rodino D, Rodolfi M, Castiglione S, Picco AM, et al. Genetic structure of Pyricularia grisea (Cooke) Sacc. isolates from Italian paddy fields. J Phytopathol 2005;153:80-6.
Ashkani S, Rafii MY, Shabanimofrad M, Miah G, Sahebi M, Azizi P, et al. Molecular breeding strategy and challenges towards improvement of blast disease resistance in rice crop. Front Plant Sci 2015;6:886.
Merzaeva OV, Shirokikh IG. Colonization of plant rhizosphere by actinomycetes of different genera. Microbiology 2006;75:226-30.
Thilagam R, Hemalatha N. Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil Actinobacteria. J Appl Microbiol 2019;126:1835-49.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.