EVALUATION OF THE ANALGESIC ACTIVITY OF AQUEOUS AND ALCOHOLIC EXTRACT OF FLOWERS OF PLUMERIA ALBA LINN IN EXPERIMENTAL ANIMALS

  • SATHIYA VINOTHA AT Department of Pharmacology, KMCH Institute of Health Sciences and Research, Coimbatore, Tamil Nadu, India.
  • UMAMAGESWARI MS Department of Pharmacology, KMCH Institute of Health Sciences and Research, Coimbatore, Tamil Nadu, India.
  • UMAMAHESWARI A Department of Pharmacology, PSG Institute of Medical Sciences and Research, Coimbatore.
  • VELARUL S Department of Pharmacology, KMCH Institute of Health Sciences and Research, Coimbatore, Tamil Nadu, India.

Abstract

Objective: Plumeria alba (Apocynaceae), commonly known as “Frangipani” is an important medicinal plant, widely distributed throughout Southern India. In the traditional medicinal system, different parts of this plant have been mentioned to be useful in a wide variety of diseases. In this study, analgesic activity of aqueous and alcoholic extract of flowers of P. alba Linn was evaluated by hot-plate and acetic acid-induced writhing methods to substantiate and expand its clinical applications.


Methods: The flowers of P. alba were collected from the local area in and around Coimbatore (India). The flowers were dried, powdered, and were extracted using soxhlet apparatus for 12 h using water and 95% of ethanol as a solvent. The test extracts were administered orally at a dose of 100 & 200 mg/kg while diclofenac sodium (10 mg/kg) served as standard.


Results: The ethanolic flower extract of 200 mg/kg had significant analgesic activity in acetic acid-induced abdominal writhing response (***p<0.001) when compared to control and standard drug diclofenac. It also exhibited significant analgesic activity (***p<0.001) by increasing the pain reaction time of the rats in comparison to control and standard in hot plate method.


Conclusion: The results suggest that ethanolic flower extracts of P. alba possess potent analgesic properties, which support its use in traditional medicine and suggesting that the plant should be further investigated for its pharmacological active natural products.

Keywords: Analgesic activity, Acetic acid-induced writhing method, Hot-plate, Plumeria alba

References

1. Sherrington S. The Integrative Action of Nervous System. United Kingdom: Yale University Press; 1920.
2. International Association for the Study of Pain, IASP Taxonomy; 2015. Available from: https://www.iasp-pain.org/education/content.aspx. itemnumber=1698. [Last accessed on 2015 Jan 23].
3. Sang CN. NMDA receptor antagonists in neuropathic pain: Experimental methods to clinical trials. J Pain Symptom Manage 2000;19 Suppl 1:21-5.
4. Shinde PR, Patil PS, Bairagi V. A phytopharmacological review of Plumeria species. Sch Acad J Pharm 2014;3:217-27.
5. Devprakash D, Tembare R, Gurav S, Kumar GP, Tamizhmani T. A review of phytochemical constituents and pharmacological activity of Plumeria species. Int J Curr Pharm Res 2012;4:1-6.
6. Gaur K, Rana C, Nema RK, Kori ML, Sharma CS. Anti-inflammatory and analgesic activity of hydro-alcoholic leaves extract of Euphorbia neriifolia Linn. Asian J Pharm Clin Res 2009;2:26-9.
7. Tessou KZ, Lawson-Evi P, Metowogo K, Diallo A, Eklu-Gadegkeku K, Aklikokou K, et al. Acute and sub-acute toxicity studies of Plumeria alba Linn. (Apocynaceae) hydroalcoholic extract in rat. Int J Biomed Sci 2013;9:255-9.
8. Gawade SP. Acetic acid induced painful endogenous infliction in writhing test on mice. J Pharmacol Pharmacother 2012;3:348.
9. Fan SH, Ali NA, Basri DF. Evaluation of analgesic activity of the methanol extract from the galls of Quercus infectoria (Olivier) in Rats. Evid Based Complement Alternat Med 2014;2014:976764.
10. Rajagopal MR. Pain-basic consideration. Indian J Anaesth 2006;50:332-4.
11. Le Bars D, Adam F. Nociceptors and mediators in acute inflammatory pain. Ann Fr Anesth Reanim 2002;21:315-35.
12. Zaheer Z, Konale AG, Patel KA, Khan S, Ahmed RZ. Comparative phytochemical screening of flowers of Plumeria alba and Plumeria rubra. Asian J Pharm Clin Res 2010;3:88-9.
13. Barua CC, Roy JD, Buragohain B, Barua AG, Borah P, Lahkar M. Analgesic and anti-nociceptive activity of hydroethanolic extract of Drymaria cordata Willd. Indian J Pharmacol 2011;43:121-5.
14. Anggoro B, Istyastono E, Hariono M. Future molecular medicine from white frangipani (Plumeria alba L.): A review. J Med Plants Res 2020;14:544-54.
15. Choudhary M, Kumar V, Gupta P, Singh S. Investigation of antiarthritic potential of Plumeria alba L. leaves in acute and chronic models of arthritis. Biomed Res Int 2014;2014:474616.
16. Engineer AS, Pal S, Desai KR, Highland HN. Synergistic antioxidant action of Murraya koenigii and Plumeria rubra extracts enhance hepatoprotective efficacy against methotrexate induced liver injury. Int J Pharm Pharm Sci 2017;9:152-9.
17. Yadav AV, Upasani CD. Nephroprotective activity of Plumeria rubra against cisplatin induced nephrotoxicity in experimental rats. Int J Pharm Pharm Sci 2019;11108-13.
18. Sura J, Dwivedi S, Dubey R. Pharmacological, phytochemical, and traditional uses of Plumeria alba Linn. an Indian medicinal plant. J Pharm Biosci 2016;1:14-7.
19. Radha R, Sivakumar T, Arokiyaraj S. Pharmacognostical evaluation of Plumeria alba Linn. Res J Pharm Technol 2008;1:496-501.
20. Sumeet D, Abhishek D, Dwivedi SN. Folk lore uses of some plants by the tribes of Madhya Pradesh with special reference to their conservation. Ethnobot Leaflets 2008;12:741-3.
Statistics
4 Views | 9 Downloads
Citations
How to Cite
VINOTHA AT, S., U. MS, U. A, and V. S. “EVALUATION OF THE ANALGESIC ACTIVITY OF AQUEOUS AND ALCOHOLIC EXTRACT OF FLOWERS OF PLUMERIA ALBA LINN IN EXPERIMENTAL ANIMALS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 4, Apr. 2021, pp. 172-4, doi:10.22159/ajpcr.2021.v14i4.40644.
Section
Original Article(s)