HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ESTIMATION METHOD FOR TRANSDERMAL DIFFUSION STUDY OF GRANISETRON
Keywords:Reversed-phase high-performance liquid chromatography, Flux, Transdermal, Porcine ear, Granisetron
Objective: The study is aimed to develop a simple, isocratic and sensitive reversed-phase high-performance liquid chromatography method for the analysis of granisetron during transdermal permeation studies through porcine ear epithelium.
Methods: A reversed-phase (C18) column was used with ultraviolet detection at 210 nm. Selected mobile phase contained 25% (v/v) of acetonitrile and 75% (v/v) of 0.25 mM potassium dihydrogen orthophosphate solution pH adjusted to 3.0 using 1% orthophosphoric acid solution.
Results: Calibration curve showed good linearity over 0.1–30 μg/mL concentration range of porcine ear permeates in 80% ethanol and 20% water (blank permeates). The applicability of the method was demonstrated by the analysis of diffusion study samples of granisetron through porcine ear epithelium and the steady-state permeability flux (J) from ethanolic solution was found to be 4.707 µg/square cm/h.
Conclusion: The developed method can be used in the analysis of diffusion study samples of granisetron transdermal formulations through the porcine ear epithelium.
Hsu ES. A review of granisetron, 5-hydroxytryptamine3 receptor antagonists, and other antiemetics. Am J Ther 2010;17:476-86.
Schnell FM. Chemotherapy-induced nausea and vomiting: The importance of acute antiemetic control. Oncologist 2003;8:187-98.
Julie LR. Treatment of chemotherapy-induced nausea in cancer patients. Eur Oncol 2010;6:14-6.
Jacobi U, Kaiser M, Toll R, Mangelsdorf S, Audring H, Otberg N, et al. Porcine ear skin: An in vitro model for human skin. Skin Res Technol 2007;13:19-24.
Dick IP, Scott RC. Pig ear skin as an in-vitro model for human skin permeability. J Pharm Pharmacol 1992;44:640-5.
Ohtaka R, Maeda M, Iwagami T, Ueda T, Kimura Y, Imai K, et al. Precision of internal standard method in HPLC analysis. Yakugaku Zasshi 2003;123:349-55.
Krishnaiah YS, Al-Saidan SM, Chandrasekhar DV, Rama B. Effect of nerodilol and carvone on in vitro permeation of nicorandil across rat epidermal membrane. Drug Dev Ind Pharm 2008;32:423-35.
Therapeutic Goods Administration. Australian Public Assessment Report for Granisetron. Australia: Therapeutic Goods Administration; 2011. p. 1-60. Available from: https://www.tga.gov.au/node/799.
Ranade VV. Drug delivery systems. 6. Transdermal drug delivery. J Clin Pharmacol 1991;31:401-18.
Johnson ME, Blankschtein D, Langer R. Evaluation of solute permeation through the Stratum corneum: Lateral bilayer diffusion as the primary transport mechanism. J Pharm Sci 1997;86:1162-72.
Lee CK, Uchida T, Kitagawa K, Yagi A, Kim NS, Goto S. Skin permeability of various drugs with different lipophilicity. J Pharm Sci 1994;83:562-5.
Assessment Report: Sancuso, INN-granisetron. Vol. 44; 2012. Available from: http://www.ema.europa.eu/docs/en_GB/document_library/ EPAR_-_Public_assessment_report/human/002296/WC500127130.pdf.
Alkilani AZ, McCrudden MT, Donnelly RF. Transdermal drug delivery: Innovative pharmaceutical developments based on disruption of the barrier properties of the Stratum corneum. Pharmaceutics 2015;7:438-70.
Woo JS. Nasal absorption studies of granisetron in rats using a validated high-performance liquid chromatographic method with mass spectrometric detection. Arch Pharm Res 2006;30:778-84.
Zheng WW, Zhao L, Wei YM, Ye Y, Xiao SH. Preparation and the in vitro evaluation of nanoemulsion system for the transdermal delivery of granisetron hydrochloride. Chem Pharm Bull (Tokyo) 2010;58:1015-9.
Zhao N, Cun D, Li W, Ma X, Sun L, Xi H, et al. In vitro percutaneous absorption enhancement of granisetron by chemical penetration enhancers. Drug Dev Ind Pharm 2013;39:561-8.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.