• SENTHIL KUMARI C Department of Pharmacology, K.K. College of Pharmacy, The Tamil Nadu Dr. M.G.R Medical University, Chennai, Tamil Nadu, India.
  • DHANASEKHAR KESAVELU Department of Paediatric Gastroenterology, Apollo Children’s Hospital, Tamil Nadu, India.



Mirabilis jalapa, Hematological parameters, Histological changes, Toxicity studies


Objective: The objective of the study was to evaluate the toxicological potential of the ethanolic extract of leaves of Mirabilis jalapa linn through acute and subacute toxicity studies in albino Wistar rats.

Methods: For acute toxicity studies, the ethanolic extract of M. jalapa was given up to 2000 mg/kg and then the animals were observed for 14 days to find out any adverse effect or death. For sub-acute toxicity studies, the exact was given for 28 days and the following parameters were observed such as changes in body weight, food intake, water intake, hematological parameters, biochemical parameters, lipid profile, urine analysis, and histopathological studies were undertaken.

Results: Single oral administration of 2000 mg/kg of the ethanolic extract of M. jalapa produced no mortality or signs of toxicity. During subacute toxicity there were no changes in body weight, food intake and water intake were observed. There were no changes in lipid profile, hematological parameters, and biochemical parameters. In histopathological changes, there were no structural changes in treated groups when compared to control.

Conclusion: The leaves of ethanolic extract of M. jalapa is safe when administered for 28 days.

There were no deaths or signs of toxicity in treated rats during acute toxicity studies and subacute toxicity studies.


Download data is not yet available.


Liya FI, Yasmin MF, Chowdhury NS. Mirabilis jalapa: A review of ethno and pharmacological activities. Adv Med Plant Res 2021;9:1-10.

Nidavani RB, Mahalakshmi AM. An ethanopharmacological review of four o’clock flower plant (Mirabilis jalapa Linn). J Biol Sci Opin 2014;6:344-8.

Saha S, Deb J, Deb NK. Review on Mirabilis jalapa L., (nyctaginaceae): A medicinal plant. Int J Herbal Med 2020;8:14-8.

Singh M, Garg A, Mittal SK, Kalia AN. Mirabilis jalapa-a review. Int J Pharm Chem Appl Sci 2012;1:1-22.

Satheeskumar KG. Significant role of Soxhlet extraction process in phytochemical. Mintage J Pharm Med Sci 2018;7:43-7.

Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromatic Plants 2015;4:2-4.

Ekanayake CP, Thammitiyagodage MG, Padumadasa S, Seneviratne B, Padumadasa C. Abeysekera AM. Acute and subacute toxicity studies of the ethyl acetate soluble proanthocyanidins of the immature inflorescence of Cocos nucifera L. in female Wistar rats. Biomed Res Int 2019;2019:8428304.

OECD Ilibrary, OECG Guidelines for the Testing of Chemicals Section 4; 2008.

Hazel AM, Pattarayan R, Banumathy V. Acute and sub-acute (28-days) oral toxicity studies of Eraippu noi chooranam. Int J Adv Res Biol Sci 2016;3:106-12.

Kharchoufa L, Bouhrim M, Bencheikh N, El Assri S, Amirou A, Yamani A, et al. Acute and sub-acute toxicity studies of the aqueous extract from Haloxylon scoparium pomel (Hammada scoparia (Pomel)) by oral administration in rodents. Biomed Res Int 2020;2020:4020647.

Olaniyan JM, Muhammad HL, Makun HA, Busari MB, Abdullah AS. Acute and sub-acute toxicity studies of aqueous and methane extracts of Nelsonia campestris in rats. J Acute Dis 2016;1:62-70.

Gandhare B, Kavimani S, Rajkapoor B. Acute and sub-acute toxicity study of methanolic extract of Ceiba pendtandra (Linn) Gaertn. On rats. J Sci Res 2013;5:315-24.

Ghadirkhomi A, Safaeian L, Zolfaghari B, Agha Ghazvini MR, Rezaei P. Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats. Avicenna J Phytomed 2016;6:558-66.

Patel SS, Verma S, Nayak G, Singhai AK, Ganesh N. Acute and sub-acute toxicity studies of Passiflora nepalensis in rats. Rev Bras Farmacogn 2011;21:730-6.



How to Cite

C, S. K., and D. KESAVELU. “ACUTE AND SUBACUTE TOXICITY STUDIES OF ETHANOLIC EXTRACT OF MIRABILIS JALAPA LINN IN WISTAR ALBINO RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 14, no. 6, July 2021, pp. 80-85, doi:10.22159/ajpcr.2021.v14i6.40979.



Original Article(s)