EVALUATION OF IN VITRO ANTICANCER ACTIVITY OF AERIAL PARTS OF AVICENNIA ALBA PLANT METHANOLIC EXTRACT AGAINST HELA AND MCF-7 CELL LINES
Keywords:Avicennia alba, Cisplatin, HeLa cell lines, MCF-7cell lines, 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide assay
Objective: The current study emphasizes the effects of stem and leaf methanolic extract of mangrove plant Avicennia alba on human tumor cell lines HeLa and MCF-7.
Method: The collected mangrove plant sample was subjected to extraction with methanol and screened for anticancer activity according to 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay method. The ability of the plant extract to inhibit the proliferation of HeLa and MCF-7 cell lines procured from NCCS Pune was compared parallel to inhibitory effect of standard drug Cisplatin. The spectrophotometric optical densities obtained were plotted on graph and consecutive data were obtained to depict the inhibitory effect and IC50 values. The histopathology studies were also done for a clear insight to understand the depth of inhibition microscopically.
Results: The MTT assay performed illustrates the plant samples with different concentrations (10, 20, 25, 30, and 50 μg) showed significant inhibitory effect on comparison with the standard drug Cisplatin. The IC50 values and the inhibitory OD values elucidate effective projection of plant as a potential source of anticancer activity based on comparative study. The histopathology studies revealed an insight to a clearer picture of how the cells growth was controlled. It was found that the methanolic extract of A. alba exhibited more activity against HeLa cell lines than MCF-7 cell lines.
Conclusion: The synergistic effect of various phytochemicals present in the methanolic plant extract played a significant role in establishing the plant as a significant anticancer source.
Dikshit A, Shahi SK, Pandey KP, Patra M, Shukla AC. Aromatic plants: A source of natural chemo-therapeutants. Natl Acad Sci Lett 2004;27:145-64.
Larkin T. Herbs are Often More Toxic than Magical. United States: Department of Health and Human Services, Public Health Service, Food and Drug Administration; 1984.
Newman DJ, Cragg GM, Snader KM. The influence of natural products upon drug discovery. Nat Prod Rep 2000;17:215-34.
Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981-2002. J Nat Prod 2003;66:1022-37.
Abu-Dahab R, Afifi F. Antiproliferative activity of selected medicinal plants of Jordan against a breast adenocarcinoma cell line (MCF7). Sci Pharm 2007;75:121-46.
Priya K, Krishnakumari S, Vijayakumar M. Cyathula prostrata: A potent source of anticancer agent against daltons ascites in Swiss albino mice. Asian Pac J Trop Med 2013;6:776-9.
Alison MR. Cancer. Encyclopedia of Life Sciences. London: Nature Publishing Group; 2001.
Wu J, Xiao Q, Xu J, Li MY, Pan JY, Yang MH. Natural products from true mangrove flora: Source, chemistry and bioactivities. Nat Prod Rep 2008;25:955-81.
Jain R, Jain SK. Screening of in vitro cytotoxic activity of some medicinal plants used traditionally to treat cancer in Chhattisgarh state, India. Asian Pac J Trop Biomed 2011;1:S147-50.
Mulla SK, Swamy PA. Anticancer activity of ethanol and polyphenol extracts of Portulaca quadrifida Linn. On human colon cancer cell lines. Int J Pharm Biosci 2012;3:488-98.
Duke N, Ball M, Ellison J. Factors influencing biodiversity and distributional gradients in mangroves. Glob Ecol Biogeogr Lett 1998;7:27-47.
Kathiresan K. Biodiversity of Mangrove Ecosystems, Proceedings of Mangrove Workshop. Gujarat, India: GEER Foundation; 2008.
Tomlinson PB. The Botany of Mangroves. London: Cambridge University Press; 1986.
Kathiresan K, Ramanathan T. Medicinal Plants of Parangipettai Coast. Chennai: Annamalai University; 1997.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Doyle A, Griffiths JB, editors. Cell and Tissue Culture for Medical Research. United States: Wiley; 2000.
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