Antidiabetic and antihyperlipidemic activities of a novel polyherbal formulation in streptozotocin - nicotinamide induced diabetic wistar rats

  • KISHOR KUMAR V Department of Pharmacognosy, J K K Nattraja College of Pharmacy, B. Kumarapalayam, Namakkal District, Tamil Nadu, India.
  • LALITHA K G Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai, Tamil Nadu, India


Objective: To investigate the antidiabetic and antihyperlipidemic activities of polyherbal formulation (PHF) containing seven plants namely Cassia auriculata, Cassia fistula, Syzygium cumini, Cyperus rotundus, Saussurea lappa, Terminalia arjuna and Salacia reticulate in streptozotocin (STZ) - nicotinamide (NC) induced diabetic rats by administering oral doses (200 and 400 mg/kg body weight).

Materials and Methods: Animals were divided into diabetic and nondiabetic groups. Rats were fed with normal laboratory diet and induced with a single intraperitoneal injection of 60mg/kg of STZ, and thereafter 120 mg/kg NC was injected after 15min. Diabetic rats were treated with formulation (200 and 400 mg/kg) and glibenclamide 5 mg/kg. Blood glucose levels were measured using blood glucose test strips with ACCU CHEK glucometer. Glycosylated haemoglobin, total haemoglobin, lipid profiles, lipoproteins, hepatic marker enzymes activity were determined in normal and STZ- NC induced diabetic rats after oral administration of the PHF for 28 days. Histopathological changes in normal and diabetic rat pancreas organs were also observed after PHF treatment.The statistical analysis of results was carried out using one-way analysis (ANOVA) followed by post hoc multiple comparison tests.

Results: Treatment of diabetic rats with PHF (200 and 400 mg/kg) and glibenclamide (5 mg/kg) indicate significant decreased blood glucose level and significant improvement in body weight. PHF treated rats showed significant (P < 0.01) decrease in the level of HbA1C, TC, TG, LDL, VLDL, AST, ALT and ALP while a significant increment in the level of Hb, HDL cholesterol was observed. Furthermore, the PHF treated rats has a favourable effect on the histopathological changes of the pancreas in STZ-NC induced diabetes.

Conclusion: These findings suggested the antihyperglycemic and antihyperlipidemic properties of the PHF and thus help in preventing future complications of diabetes.

Keywords: nil


1. Yeh GY, Eisenberg DM, Kaptchuk TJ, Phillips RS. Systematic review
of herbs and dietary supplements for glycemic control in diabetes.
Diabetes Care 2003;26:1277-94.
2. Srivastava S, Lal VK, Pant KK. Polyherbal formulations based on Indian
medicinal plants as antidiabetic phytotherapeutics. Phytopharmacology
3. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. New
Delhi: Springer Publication; 2004. p. 127-8, 637.
4. Sutalangka C, Wattanathorn J. Neuroprotective and cognitiveenhancing
effects of the combined extract of Cyperus rotundus and
Zingiber officinale. BMC Complement Altern Med 217;17:135.
5. Birder S, Kangralkar VA, Mandavkar Y, Thakur M, Chougule N. Antiinflammatory,
anti-arthritic, analgesic and anticonvulsant activity of
Cyperus essential oils. Int J Pharm Pharm Sci 2010;2:128-30.
6. Abd Eldaim MA, Tousson E, El Sayed IT, Awd WM. Ameliorative effects
of Saussurea lappa root aqueous extract against ethephon-induced
reproductive toxicity in male rats. Environ Toxicol 2019;34:150-9.
7. Ansari S, Siddiqui MA, Malhotra S, Maaz M. Antiviral efficacy of qust
(Saussurea lappa) and afsanteen (Artemisia absinthium) for chronic
hepatitis B: A prospective single-arm pilot clinical trial. Pharmacogn
Res 2018;10:282-90.
8. Ragavan B, Krishnakumari S. Antidiabetic effect of T. arjuna bark
extract in alloxan induced diabetic rats. Indian J Clin Biochem
9. Biswas M, Kar B, Bhattacharya S, Kumar RB, Ghosh AK, Haldar PK.
Antihyperglycemic activity and antioxidant role of Terminalia arjuna
leaf in streptozotocin-induced diabetic rats. Pharm Biol 2011;49:335-40.
10. Yoshino K, Miyauchi Y, Kanetaka T, Takagi Y, Koga K. Anti-diabetic
activity of a leaf extract prepared from Salacia reticulata in mice.
Biosci Biotechnol Biochem 2009;73:1096-104.
11. Siddha Formulary of India, Part 1.
12. Selvamani P, Latha S, Elayaraja K, Babu PS, Gupta JK, Pal TK,
et al. Antidiabetic activity of the ethanol extract of Capparis sepiaria L
leaves. Indian J Pharm Sci 2008;70:378-80.
13. Organization for Economic Cooperation and Development. OECD
Guidelines for Testing of Chemicals. Section 4, Health Effects: TestNo. 425: Acute Oral Toxicity: Up-and-Down Procedure. France: OECD
Publishing; 2006. p. 1-27. Available from: htt://www.oecdbookshop.
org/oecd/index.asp/langen. [Last accessed on 2006 Mar 23].
14. Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-
Buys D, et al. Experimental NIDDM: Development of a new model in
adult rats administered with streptozotocin and nicotinamide. Diabetes
15. Sam I, Punitha R, Shirwaikar A, Shirwaikar A. Antidiabetic activity of
benzyl tetra isoquinoline alkaloid berberine in streptozotocin-nicotinamide
induced Type 2 diabetic rats. Diabetol Croat 2005;34:117-28.
16. Annie S, Rajendran K, Sam I, Punitha R. Antidiabetic activity of
alcoholic stem extract of Coscinium fenestratum in streptozotocinnicotinamide
induced Type 2 diabetic rats. J Ethnopharmacol
17. Kumar EK, Janardhana GR. Antidiabetic activity of alcoholic stem
extract of Nervilia plicata in streptozotocin-nicotinamide induced Type
2 diabetic rats. J Ethnopharmacol 2011;133:480-3.
18. Kumar S, Kumar V, Prakash O. Antidiabetic and antilipemic effects of
Cassia siamea leaves extract in streptozotocin induced diabetic rats.
Asian Pac J Trop Med 2010;3:871-3.
19. Babu V, Gangadevi T, Subramoniam A. Antidiabetic activity of ethanol
extract of Cassia kleinii leaf in streptozotocin-induced diabetic rats and
isolation of an active fraction and toxicity evaluation of the extract.
Indian J Pharmacol 2003;35:290-6.
20. Reitman S, Frankel S. Colorimetric method for the determination of
serum glutamate oxalo-acetate and glutamate pyruvate transaminase.
Am J Clin Pathol 1957;28:56-63.
21. Selvan VT, Manikandan L, Kumar GP, Suresh R, Kakoti BB,
Gomathi P, et al. Antidiabetic and antioxidant effect of methanol extract
of Artanema sesamoides in streptatozocin-induced diabetic rats. Int J
Appl Res Nat Prod 2008;1:25-33.
22. Adisa RA, Choudhary MI, Olorunsogo OO. Hypoglycemic activity of
Buchholzia coriacea (Capparaceae) seeds in streptozotocin-induced
diabetic rats and mice. Exp Toxicol Pathol 2011;63:619-25.
23. Nastaran JS. Antihyperglycaemia and antilipidaemic effect of Zizipus
vulgaris L on streptozotocin induced diabetic adult male Wister rats.
Physiol Pharmacol 2011;47:219 -23.
24. Arunachalam K, Parimelazhagan T. Antidiabetic activity of Ficus
amplissima Smith bark extract in streptozotocin induced diabetic rats. J
Ethnopharmacol 2013;147:302-10.
25. Swanston-Flatt SK, Day C, Flatt PR, Gould BJ, Bailey CJ. Glycaemic
effects of traditional European plant treatments for diabetes. Studies in
normal and streptozotocin diabetic mice. Diabetes Res 1989;10:69-73.
26. Deguchi Y, Miyazaki K. Anti-hyperglycemic and anti-hyperlipidemic
effects of guava leaf extract. Nutr Metab 2010;7:9.
27. Ananthan R, Latha M, Ramkumar KM, Pari L, Baskar C, Bai VN.
Effect of Gymnema montanum leaves on serum and tissue lipids in
alloxan diabetic rats. Exp Diabesity Res 2003;4:183-9.
28. Kasetti RB, Rajasekhar MD, Kondeti VK, Fatima SS, Kumar EG,
Swapna S, et al. Antihyperglycemic and antihyperlipidemic activities
of methanol: Water (4:1) fraction isolated from aqueous extract of
Syzygium alternifolium seeds in streptozotocin induced diabetic rats.
Food Chem Toxicol 2010;48:1078-84.
29. Lenzen S. The mechanism of alloxan and streptozotocin induced
diabetes. Diabetologia 2008;51:216-26.
30. Shanmugasundaram ER, Gopinath KL, Shanmugasundaram KR,
Rajendran VM. Possible regeneration of the islets of langerhans in
streptozotocin-diabetic rats given Gymnema sylvestre leaf extracts. J
Ethnopharmacol 1990;30:265-79.
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KISHOR KUMAR V, and LALITHA K G. “Antidiabetic and Antihyperlipidemic Activities of a Novel Polyherbal Formulation in Streptozotocin - Nicotinamide Induced Diabetic Wistar Rats”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 4, Apr. 2021, pp. 81-86, doi:10.22159/ajpcr.2021v14i4.41523.
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