ECLIPTA ALBA (BHRINGRAJ): A PROMISING HEPATOPROTECTIVE AND HAIR GROWTH STIMULATING HERB.

ISHA KUMARI; HEMLATA KAURAV; GITIKA CHAUDHARY

doi:10.22159/ajpcr.2021.v14i7.41569

Authors

ISHA KUMARI Shuddhi Ayurveda, Jeena Sikho Lifecare Pvt. Ltd. Zirakpur, Punjab, India.
HEMLATA KAURAV Shuddhi Ayurveda, Jeena Sikho Lifecare Pvt. Ltd. Zirakpur, Punjab, India.
GITIKA CHAUDHARY Shuddhi Ayurveda, Jeena Sikho Lifecare Pvt. Ltd. Zirakpur, Punjab, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i7.41569

Keywords:

Bhringraj, Rasapanchak, Coumestans, Saponin, Hepatoprotective

Abstract

Plants are the primary source of food, shelter, and various remedial approaches. They are being in use for treating various kinds of human ailments across the world since the ancient times. One of such important plants is Eclipta alba hassk (Asteraceae) which is commonly known as Bhringraj and false daisy. This plant is known for its extra-ordinary therapeutic properties. It is one of most widely used plant in traditional systems of medicines such as Ayurvedic, Unani, Sidha, homeopathy, Chinese, and folk. Each part of this medicinal plant contains many important phytochemical constituents such as coumestans, triterpenes, flavonoids, steroids, polypeptides, and saponins. It is majorly used for enhancing the growth, strength, and blackening of hair. It is used as the main ingredient in many hair oils. It significantly treats problems related to hair such as hair fall, dandruff, premature greying, and baldness. E. alba exhibits many other important biological properties such as anticancer, anti-bacterial, anti-viral, anti-stress, and immunomodulatory. Thus, this medicinal plant is playing a vital role in the medical field and cosmetic industry. The present review is a summary of phytomedicinal importance of E. alba in Ayurveda as well as folk medicine system.

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References

Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol 2005;29:41-7.

Ghorbani A. Studies on pharmaceutical ethnobotany in the region of Turkmen Sahra, north of Iran: (Part 1): General results. J Ethnopharmacol 2005;102:58-68. DOI: https://doi.org/10.1016/j.jep.2005.05.035

Chukwuma EC, Soladoye MO, Feyisola RT. Traditional medicine and the future of medicinal Plants in Nigeria. J Med Plants Stud 2015;3:23-9.

Lifongo LL, Simoben CV, Ntie-Kang F, Babiaka SB, Judson PN. A bioactivity versus ethnobotanical survey of medicinal plants from Nigeria, West Africa. Nat Prod Bioprospect 2014;4:1-9. DOI: https://doi.org/10.1007/s13659-014-0005-7

Akintonwa A, Awodele O, Afolayan G, Coker HA. Mutagenic screening of some commonly used medicinal plants in Nigeria. J Ethnopharmacol 2009;125:461-70. DOI: https://doi.org/10.1016/j.jep.2009.07.013

Ayyanar M, Ignacimuthu S. Ethnobotanical survey of medicinal plants commonly used by Kani tribals in Tirunelveli hills of Western Ghats, India. J Ethnopharmacol 2011;134:851-64. DOI: https://doi.org/10.1016/j.jep.2011.01.029

Yadav RN, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol 2011;3:10-4. DOI: https://doi.org/10.5897/AJB11.1948

Simmler C, Graham JG, Chen SN, Pauli GF. Integrated analytical assets aid botanical authenticity and adulteration management. Fitoterapia 2018;129:401-14. DOI: https://doi.org/10.1016/j.fitote.2017.11.017

Ichim MC. The DNA-based authentication of commercial herbal products reveals their globally widespread adulteration. Front Pharmacol 2019;10:1227. DOI: https://doi.org/10.3389/fphar.2019.01227

Nagabhushan RK, Raveesha A. Ethnobotanical survey and scientific validation of medicinal plants used in the treatment of fungal infections in Agumbe region of Western Ghats, India. Int J Pharm Pharm Sci 2015;7:273-7.

Yorek N, Aydin H, Ugulu I, Dogan Y. An investigation on students’ perceptions of biodiversity. Online Submission 2008;7:175-84.

Ateş DA, Turgay Ö. Antimicrobial activities of various medicinal and commercial plant extracts. Turk J Biol 2003;27:157-62.

Newman DJ, Cragg GM, Snader KM. The influence of natural products upon drug discovery. Nat Prod Rep 2000;17:215-34. DOI: https://doi.org/10.1039/a902202c

Abu-Shanab B, Adwan GM, Abu-Safiya D, Jarrar N, Adwan K. Antibacterial activities of some plant extracts utilized in popular medicine in Palestine. Turk J Biol 2005;28:99-102.

Hill AF. Economic botany. In: A Textbook of Useful Plants and Plant Products Economic Botany. London: McGraw-Hill Publishing Co. Ltd.; 1937.

Foster BC, Arnason JT, Briggs CJ. Natural health products and drug disposition. Annu Rev Pharmacol Toxicol 2005;45:203-26. DOI: https://doi.org/10.1146/annurev.pharmtox.45.120403.095950

Akinyemi B. Recent concept in plaque formation. J Clin Pathol 2000;30:13-6.

Calixto JB. Twenty-five years of research on medicinal plants in Latin America: A personal view. J Ethnopharmacol 2005;100:131-4. DOI: https://doi.org/10.1016/j.jep.2005.06.004

Sahito SR, Memon MA, Kazi TG, Kazi GH, Jakhrani MA, Haque QU, et al. Evaluation of mineral contents in medicinal plant Azadirachta indica (Neem). J Chem Soc Pak 2003;25:139-43.

Sukumaran S, Raj AD. Medicinal plants of sacred groves in Kanyakumari district Southern Western Ghats. Vol. 9. Council of Scientific and Industrial Research; 2010. p. 294-9.

Abd El-Ghani MM. Traditional medicinal plants of Nigeria: An overview. Agric Biol J North Am 2016;7:220-47.

Verma S, Singh SP. Current and future status of herbal medicines. Vet World 2008;1:347. DOI: https://doi.org/10.5455/vetworld.2008.347-350

Singh AP. Promising phytochemicals from Indian medicinal plants. Ethnobot Leaf 2005;2005:18.

Bussmann RW, Gilbreath GG, Solio J, Lutura M, Lutuluo R, Kunguru K, et al. Plant use of the Maasai of Sekenani Valley, Maasai Mara, Kenya. J Ethnobiol Ethnomed 2006;2:1-7. DOI: https://doi.org/10.1186/1746-4269-2-22

Okoli RI, Aigbe O, Ohaju-Obodo JO, Mensah JK. Medicinal herbs used for managing some common ailments among Esan people of Edo State, Nigeria. Pak J Nutr 2007;6:490-6. DOI: https://doi.org/10.3923/pjn.2007.490.496

Moerman DE. Medicinal Plants of Native America. Ann Arbor: Museum of Anthropology, University of Michigan Museum; 1986.

Yang L, Yang C, Li C, Zhao Q, Liu L, Fang X, et al. Recent advances in biosynthesis of bioactive compounds in traditional Chinese medicinal plants. Sci Bull 2016;61:3-17. DOI: https://doi.org/10.1007/s11434-015-0929-2

Gan RY, Kuang L, Xu XR, Zhang Y, Xia EQ, Song FL, et al. Screening of natural antioxidants from traditional Chinese medicinal plants associated with treatment of rheumatic disease. Molecules 2010;15:5988-97. DOI: https://doi.org/10.3390/molecules15095988

Zuo GY, Zhang XJ, Yang CX, Han J, Wang GC, Bian ZQ. Evaluation of traditional Chinese medicinal plants for anti-MRSA activity with reference to the treatment record of infectious diseases. Molecules 2012;17:2955-67. DOI: https://doi.org/10.3390/molecules17032955

Gen XP. Medicinal Plants: The Chinese Approach. Vol. 7. Geneva: World Health Organization Forum; 1986. p. 84-5.

Hostettmann K, Marston A. Twenty years of research into medicinal plants: Results and perspectives. Phytochem Rev 2002;1:275-85. DOI: https://doi.org/10.1023/A:1026046026057

Green EC, Makhubu L. Traditional healers in Swaziland: Toward improved cooperation between the traditional and modern health sectors. Soc Sci Med 1984;18:1071-9. DOI: https://doi.org/10.1016/0277-9536(84)90167-9

Chokotia LS, Vashistha P, Sironiya R, Matoli H. Pharmacological activities of Eclipta alba (L.). Int J Res Dev Pharm Life Sci 2013;2:499- 502.

Puri HS. Rasayana: Ayurvedic herbs for longevity and rejuvenation: Volume 2 of traditional herbal medicines for modern times. J Altern Complement Med 2003;2:331-2. DOI: https://doi.org/10.1089/10755530360623446

Dalal S, Rana S, Sastry K, Kataria S. The Wealth of India. Vol. 3. New Delhi: Raw Materials, Council of Scientific and Industrial Research; 1952. p. 127-8.

Kumar YA, Kumar KR, Sirisha Y, Gopikrishna B. In vitro anti-cancer activity of ethanolic extract of Eclipta alba (L.). ??? 2018;6:79-87. DOI: https://doi.org/10.46624/ajphr.2018.v6.i9.006

Jahan R, Al-Nahain A, Majumder S, Rahmatullah M. Ethnopharmacological significance of Eclipta alba (L.) hassk. (Asteraceae). Int Sch Res Notices 2014;2014:1-22. DOI: https://doi.org/10.1155/2014/385969

Udayashankar AC, Nandhini M, Rajini SB, Prakash HS. Pharmacological significance of medicinal herb Eclipta alba l. a review. Int J Pharm Sci Res 2019;10:3592-606.

Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol. 3. Dehardun: International Book Distributors; 1999. p. 1616-7.

Saxena AK, Singh B, Anand KK. Hepatoprotective effects of Eclipta alba on subcellular levels in rats. J Ethnopharmacol 1993;40:155-61. DOI: https://doi.org/10.1016/0378-8741(93)90063-B

Pithayanukul P, Laovachirasuwan S, Bavovada R, Pakmanee N, Suttisri R. Anti-venom potential of butanolic extract of Eclipta prostrata against Malayan pit viper venom. J Ethnopharmacol 2004;90:347-52. DOI: https://doi.org/10.1016/j.jep.2003.10.014

Begum S, Lee MR, Gu LJ, Hossain J, Sung CK. Exogenous stimulation with Eclipta alba promotes hair matrix keratinocyte proliferation and downregulates TGF-β1 expression in nude mice. Int J Mol Med 2015;35:496-502. DOI: https://doi.org/10.3892/ijmm.2014.2022

Neamsuvan O, Ruangrit T. A survey of herbal weeds that are used to treat gastrointestinal disorders from Southern Thailand: Krabi and Songkhla Provinces. J Ethnopharmacol 2017;209:318-27. DOI: https://doi.org/10.1016/j.jep.2017.06.039

Tambe R, Patil A, Jain P, Sancheti J, Somani G, Sathaye S. Assessment of luteolin isolated from Eclipta alba leaves in animal models of epilepsy. Pharm Biol 2017;55:264-8. DOI: https://doi.org/10.1080/13880209.2016.1260597

Wagner H, Geyer B, Kiso Y, Hikino H, Rao GS. Coumestans as the main active principles of the liver drugs Eclipta alba and Wedelia calendulacea1. Plant Med 1986;52:370-4. DOI: https://doi.org/10.1055/s-2007-969188

Singh A, Duggal S, Suttee A, Singh J, Katekhaye S. Eclpita alba Linn.-ancient remedy with therapeutic potential. Int J Phytopharmacol 2010;1:57-63.

Jadhav VM, Thorat RM, Kadam VJ, Salaskar KP. Chemical composition, pharmacological activities of Eclipta alba. J Pharm Res 2002;2:1129-231.

Jain PK, Das DE. The wonder of herbs to treat-alopecia. Innov J Med Sci 2016;4:1-6.

Jadhav VM, Thorat RM, Kadam VJ, Sathe NS. Eclipta alba Linn Kesharaja: A review. J Pharm Res 2009;2:1236-41.

Mustafa K, Kanwal J, Musaddiq S, Khakwani S. Ethnobotanical and pharmacological importance of the herbal plants with anti-hair fall and hair growth activities. In: Advanced Pharmacological Uses of Medicinal Plants and Natural Products. ???: ???; 2020. p. 49-66. DOI: https://doi.org/10.4018/978-1-7998-2094-9.ch003

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi: Council of Scientific and Industrial Research; 1956.

Shekokar S, Nayak SU. A Phytopharmacological review of prospective of Bhrungaraj (Eclipta alba Hassk.). Int J Ayurvedic Med 2017;8:1-7. DOI: https://doi.org/10.47552/ijam.v8i1.892

Feng L, Zhai YY, Xu J, Yao WF, Cao YD, Cheng FF, et al. A review on traditional uses, phytochemistry and pharmacology of Eclipta prostrata (L.) L. J Ethnopharmacol 2019;245:112109. DOI: https://doi.org/10.1016/j.jep.2019.112109

Sunita P, Mishra DN. Diagnostic parameters assisted with suitable photomicrographs, pharmacognostical and phytochemical study of Eclipta prostrata (L.) L. Glob J Res Med Plants Indig Med 2016;5:292.

Jayathirtha MG, Mishra SH. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine 2004;11:361-5. DOI: https://doi.org/10.1078/0944711041495236

Mithun NM, Shashidhara S, Vivek Kumar R. Eclipta alba (L.) A review on its phytochemical and pharmacological profile. Pharmacologyonline 2011;1:345-57.

Singh A, Singh A, Dwivedi V. Effect of Eclipta alba, Azadirachta indica, Pterocarpus marsupium, Tinospora cordifolia and Mangifera indica extracts on experimentally diabetic induced rats. Int J Sci Res 2017;6:1616-20.

Soni KK, Soni S. Eclipta alba (L.) an ethnomedicinal herb plant, traditionally use in Ayurveda. J Horticult 2017;4:1-4.

Sinha SH, Raghuwanshi RI. Phytochemical screening and antioxidant potential of Eclipta prostrata (L) La valuable herb. Int J Pharm Pharm Sci 2016;8:255-60.

Thenmozhi M, Jayanthi M. Phytochemical screening and antioxidant activity of Eclipta alba L. Asian J Pharm Clin Res 2019;12:215-8. DOI: https://doi.org/10.22159/ajpcr.2019.v12i2.27828

Singh B, Saxena AK, Chandan BK, Agarwal SG, Anand KK. In vivo hepatoprotective activity of active fraction from ethanolic extract of Eclipta alba leaves. Indian J Physiol Pharmacol 2001;45:435-41.

Saxena A, Gautam S, Arya KR, Singh RK. Comparative study of phytochemicals, antioxidative potential and activity of enzymatic antioxidants of Eclipta alba and Plumbago zeylanica by in vitro assays. Free Radic Antioxid 2016;6:139-44. DOI: https://doi.org/10.5530/fra.2016.2.2

Latha BC, Ahalya S, Naidu PD, Mounica K, Kumar AR. Phytochemical evaluation of Andrographis paniculata, Cassia angustifolia and Eclipta alba. Indian J Res Pharm Biotechnol 2017;5:160-3.

Yahara S, Ding N, Nohara T, Masuda K, Ageta H. Taraxastane glycosides from Eclipta alba. Phytochemistry 1997;44:131-5. DOI: https://doi.org/10.1016/S0031-9422(96)00473-6

Zhang JS, Guo QM. Studies on the chemical constituents of Eclipta prostrata (L). Yao xue xue bao Acta Pharm Sin 2001;36:34-7.

Kaushik-Basu N, Bopda-Waffo A, Talele TT, Basu A, Costa PR, Da Silva AJ, et al. Identification and characterization of coumestans as novel HCV NS5B polymerase inhibitors. Nucl Acids Res 2008;36:1482-96. DOI: https://doi.org/10.1093/nar/gkm1178

Charpe TW, Rathod VK. Kinetics of ultrasound assisted extraction of wedelolactone from Eclipta alba. Braz J Chem Eng 2016;33:1003-10. DOI: https://doi.org/10.1590/0104-6632.20160334s20140234

Yuan HX, Zhao YL, Yan Y, Yu ZG. Studies on chemical constituents of herba ecliptae. Chin J Exp Tradit Med Form 2011;17:103-5.

Horn-Ross PL, Barnes S, Lee M, Coward L, Mandel JE, Koo J, et al. Assessing phytoestrogen exposure in epidemiologic studies: Development of a database (United States). Cancer Causes Control 2000;11:289-98. DOI: https://doi.org/10.1023/A:1008995606699

Abdel-Kader MS, Bahler BD, Malone S, Werkhoven MC, van Troon F, David, et al. DNA-damaging steroidal alkaloids from Eclipta alba from the suriname rainforest. J Nat Prod 1998;61:1202-8. DOI: https://doi.org/10.1021/np970561c

Podolak I, Galanty A, Sobolewska D. Saponins as cytotoxic agents: A review. Phytochem Rev 2010;9:425-74. DOI: https://doi.org/10.1007/s11101-010-9183-z

Khanna VG, Kannabiran K. Anticancer-cytotoxic activity of saponins isolated from the leaves of Gymnema sylvestre and Eclipta prostrata on HeLa cells. Int J Green Pharm 2009;3:227-9. DOI: https://doi.org/10.4103/0973-8258.56280

Yildirim I, Kutlu T. Anticancer agents: Saponin and tannin. Int J Biol Chem 2015;9:332-40. DOI: https://doi.org/10.3923/ijbc.2015.332.340

Kannabiran K. Antimicrobial activity of saponin fractions of the leaves of Gymnema sylvestre and Eclipta prostrata. World J Microbiol Biotechnol 2008;24:2737-40. DOI: https://doi.org/10.1007/s11274-008-9758-7

Han Y, Xia C, Cheng X, Xiang R, Liu H, Yan Q, et al. Preliminary studies on chemical constituents and pharmacological action of Eclipta prostrata L. Zhongguo Zhong Yao Za Zhi 1998;23:680-703.

Mehra PN, Nanda SS. Pharmacognosy of Bhringaraja. Antihepatotoxic drug of Indian origin. Ind J Pharm 1968;30:284.

Kaur N, Chaudhary J, Jain A, Kishore L. Stigmasterol: A comprehensive review. Int J Pharm Sci Res 2011;2:2259.

Gomathy S, Narenderan ST, Meyyanathan SN, Gowramma B. Development and validation of HPLC method for the simultaneous estimation of apigenin and luteolin in commercial formulation. J Crict Rev 2020;7:2020.

Zhang M, Chen YY, Di XH, Liu M. Isolation and identification of ecliptasaponin D from Eclipta alba (L.) hassk. Yao Xue Xue Bao Acta Pharm Sin 1997;32:633-4.

Upadhyay RK, Pandey MB, Jha RN, Pandey VB. Eclalbatin, a triterpene saponin from Eclipta alba. J Asian Nat Prod Res 2001;3:213-7. DOI: https://doi.org/10.1080/10286020108041393

Yahara S, Ding N, Nohara T. Oleanane glycosides from Eclipta alba. Chem Pharm Bull 1994;42:1336-8. DOI: https://doi.org/10.1248/cpb.42.1336

Jain S, Singh P. A dithienylacetylene ester from Eclipta-erecta Linn. Indian J Chem B Org Chem Include Med Chem 1988;27:99-100.

Lans C. Comparison of plants used for skin and stomach problems in Trinidad and Tobago with Asian ethnomedicine. J Ethnobiol Ethnomed 2007;3:1-2. DOI: https://doi.org/10.1186/1746-4269-3-3

Verma RK, Singh HN, Thakur AK, Kohli SJ. Ethnobotanical survey of medicinal and aromatic plants of Bhagalpur Region. Int J Appl Sci Biotechnol 2020;8:216-22. DOI: https://doi.org/10.3126/ijasbt.v8i2.29577

Sharma PV, Sharma G. Dhanvantari nighantu, Karveeradi varga, 4/10-11. 4th ed. Varanasi: Chaukhamba Orientalia Publication; 2005. p. 122-23.

Tripathi I. Raj Nighantu, Shatadvhadi Varga. 2nd ed. Varanasi: Chaukhamda Krushnadasa Academy; 2006. p. 89.

Sharma PV, Sharma G. Kaiyadeva Nighantu, Oshadhi varga, 1/1573- 75. 1st ed. Varanasi: Chaukhamba Orientalia Publication; 1979. p. 637.

Muddgal D. Dravyagun Vijnana. 2nd ed. Jaipur: Ayurvedic Hindi Pustak Bhandar; 2019.

Sharma P. Dravyagun Vigyan. Varanasi: Chaukambha Bharti Academy; 2019.

Saini P. Bhringraj benefits for hair and skin. Skin 2018; ???:???.

Jadhav VM, Thorat RM, Kadam VJ, Gholve SB. Kesharaja: Hair vitalizing herbs. Int J PharmTech Res 2009;1:454-67.

Pathak AP, Misar S. Management of Khalitya (Hair Fall) by Ayurvedic formulations a review. ??? 2017;2:675-80.

Pandey MK, Singh GN, Sharma RK, Lata S. Standardization of Yakrit Plihantak Churna: An ayurvedic polyherbal formulation. Int J Pharm Sci Res 2012;3:171.

Ladda PL, Magdum CS. Vitex negundo Linn.: Ethnobotany, phytochemistry and pharmacology a review. Int J Adv Pharm Biol Chem 2012;1:111-20. DOI: https://doi.org/10.7439/ijpr.v1i1.148

Tewtrakul S, Subhadhirasakul S, Cheenpracha S, Karalai C. HIV‐1 protease and HIV‐1 integrase inhibitory substances from Eclipta prostrata. Phytother Res 2007;21:1092-5. DOI: https://doi.org/10.1002/ptr.2252

Boulos L, Hadidi MN, Gohary M. Weed flora of Egypt. Cairo, Egypt: American University in Cairo Press; 1984.

Dan NV, Nhu DT. Medicinal Plants in Vietnam. Manila: WHO Regional Office for the Western Pacific; 1990.

Rai MB. Medicinal plants of Tehrathum district, Eastern Nepal. Our Nat 2003;1:42-8. DOI: https://doi.org/10.3126/on.v1i1.304

Panthi MP, Singh AG. Ethnobotany of Arghakhanchi district, Nepal: Plants used in dermatological and cosmetic disorders. Int J Appl Sci Biotechnol 2013;1:27-32. DOI: https://doi.org/10.3126/ijasbt.v1i2.8199

Khan AV, Khan AA. Ethnomedicinal uses of Eclipta prostrta Linn. Indian J Tradit Knowl 2008;7:316-20.

Parkash V, Aggarwal A. Traditional uses of ethnomedicinal plants of lower foot-hills of Himachal Pradesh-I. Indian J Tradit Knowl 2010;9:319-21.

Pandey MK, Sharma RK, Lata S. Antibacterial activity of Eclipta alba (L.) Hassk. J Appl Pharm Sci 20111;1:14.

Sardar AA, Khan Z, Perveen A, Zereen A. Appraisal of ethnobotanical uses of the wetland plants of Punjab, Pakistan. Afr J Tradit Complement Altern Med 2015;12:9-13. DOI: https://doi.org/10.4314/ajtcam.v12i4.2

Ali S, Shabbir A, Muhammad S. Ethnobotanical uses of some native and alien plants of The Jhok Reserve Forest, Punjab, Pakistan. Pak J Weed Sci Res 2018;24:89-103. DOI: https://doi.org/10.28941/24-2(2018)-4

Anwer Z, Shabbir S, Iram T, Tariq S, Murad H. Ethnobotanical study of wild flora of Haroonabad, District Bahawalnagar, Punjab, Pakistan. Eur J Biol 2020;5:41-62. DOI: https://doi.org/10.47672/ejb.564

Wariss HM, Ahmad S, Anjum S, Alam K. Ethnobotanical studies of dicotyledonous plants of Lal Suhanra national park, Bahawalpur, Pakistan. Int J Sci Res 2014;3:2452-60.

Kumar S, Das G, Shin HS, Kumar P, Patra JK. Diversity of plant species in the steel city of Odisha, India: Ethnobotany and implications for conservation of urban bio-resources. Braz Arch Biol Technol 2018;61:1-19. DOI: https://doi.org/10.1590/1678-4324-2017160650

Ahmad M, Tripathi AK, Sharma JK. Ethnobotany of important medicinal plants growing as weed and their conservation. Perspect Biodivers India 2018;4:292-6.

Singh A, Singh PK. An ethnobotanical study of medicinal plants in Chandauli district of Uttar Pradesh, India. J Ethnopharmacol 2009;121:324-9. DOI: https://doi.org/10.1016/j.jep.2008.10.018

Thirumalai T, Beverly CD, Sathiyaraj K, Senthilkumar B, David E. Ethnobotanical study of anti-diabetic medicinal plants used by the local people in Javadhu hills Tamilnadu, India. Asian Pac J Trop Biomed 2012;2:S910-3. DOI: https://doi.org/10.1016/S2221-1691(12)60335-9

Bhandari BS. Ethnobotanical plants used in health care and traditional practices by local inhabitants (Gujjars) of Rajaji Tiger Reserve, Uttarakhand, India. Indian J Tradit Knowl 2021;20:91-105. DOI: https://doi.org/10.56042/ijtk.v20i1.27596

Brinda R, Parvathy S. Ethnobotanical medicines of anaimalai union pollachi taluk, Coimbatore district, Tamilnadu. Anc Sci Life 2003;22:166.

Das H, Chakraborty U. Ethnobotanical study of medicinal plants in the Dakshin Dinajpur district. Res Rev 2019;8:18-24. DOI: https://doi.org/10.37591/rrjob.v8i3.2413

Mahajan SK. Traditional herbal remedies among the tribes of Bijagarh of West Nimar district, Madhya Pradesh. Indian J Tradit Knowl 2005;6:375-7.

Hosamani PA, Lakshman HC, Kulkarni SS, Gadi S. Documentation of ethnobotanical medicinal plants growing in rock crevices of river Kali in Dandeli wild life Sanctuary. Life Sci Leaf 2012;3:36-9.

Vijayagiri RC, Mamidala E. Ethnobotanical investigations among traditional healers in Warangal district of Andhra Pradesh, India. Pharmacogn J 2012;4:13-7. DOI: https://doi.org/10.5530/pj.2012.34.2

Patil HM, Bhaskar VV. Medicinal uses of plants by tribal medicine men of Nandurbar district in Maharashtra. Explorer 2006;5:125-30.

Mali PR. Ethnobotanical studies of Peth and Trimbakeshwar district Nashik, Maharashtra, India. Trends Life Sci 2012;1:35-7.

Sudhakar P. Ethnobotanical studies in select parks of Chennai city. India J Environ Educ 2017;17:1-33.

Ghatapanadi SR, Johnson N, Rajasab AH. Documentation of folk knowledge on medicinal plants of Gulbarga district, Karnataka. Indian J Tradit Knowl 2011;10:349-53.

Brishty SR, Setu NI, Anwar M, Jahan R, Mia MM, Kadir MF, et al. Ethnobotanical study on medicinal plants for dermatological disorders at Chittagong Hill Tracts, Bangladesh. Pharm Biomed Res 2020;6:61-90.

Rahman AH, Alam MS, Ahmad S, Naderuzzaman AT, Islam AK. An ethnobotanical portrait of a village: Koikuri, Dinajpur with reference to medicinal plants. Int J Biosci 2012;2:1-0.

Tudu D, Sinha VS. An ethnobotanical survey on medicinal plants used to mitigate anemia by tribes of east and West Singhbhum districts of Jharkhand, India. J Pharmacogn Phytochem 2017;6:2592-5.

Olatokunbo IS, Atinuke DA. Ethnobotanical survey of medicinal plants used by the indigenous people of Bayelsa state, Nigeria. Int J Eng Appl Sci Technol 2020;5:771-84. DOI: https://doi.org/10.33564/IJEAST.2020.v05i01.135

de Boer HJ, Ichim MC, Newmaster SG. DNA barcoding and pharmacovigilance of herbal medicines. Drug Saf 2015;38:611-20. DOI: https://doi.org/10.1007/s40264-015-0306-8

Grazina L, Amaral JS, Mafra I. Botanical origin authentication of dietary supplements by DNA‐based approaches. Compr Rev Food Sci Food Saf 2020;19:1080-109. DOI: https://doi.org/10.1111/1541-4337.12551

Adewunmi CO, Ojewole JA. Safety of traditional medicines, complementary and alternative medicines in Africa. Afr J Tradit Complement Altern Med 2004;1:1-3. DOI: https://doi.org/10.4314/ajtcam.v1i1.31090

Srirama R, Kumar JS, Seethapathy GS, Newmaster SG, Ragupathy S, Ganeshaiah KN, et al. Species adulteration in the herbal trade: Causes, consequences and mitigation. Drug Saf 2017;40:651-61. DOI: https://doi.org/10.1007/s40264-017-0527-0

Yee SK, Chu SS, Xu YM, Choo PL. Regulatory control of Chinese proprietary medicines in Singapore. Health Pol 2005;71:133-49. DOI: https://doi.org/10.1016/j.healthpol.2003.09.013

Miller GM, Stripp R. A study of western pharmaceuticals contained within samples of Chinese herbal/patent medicines collected from New York City’s Chinatown. Legal Med 2007;9:258-64. DOI: https://doi.org/10.1016/j.legalmed.2007.04.001

Joharchi MR, Amiri MS. Taxonomic evaluation of misidentification of crude herbal drugs marketed in Iran. Avic J Phytomed 2012;2:105.

Ernst E, Coon JT. Heavy metals in traditional Chinese medicines: A systematic review. Clin Pharmacol Ther 2001;70:497-504. DOI: https://doi.org/10.1067/mcp.2001.120249

Ernst E. Heavy metals in traditional Indian remedies. Eur J Clin Pharmacol 2002;57:891-6. DOI: https://doi.org/10.1007/s00228-001-0400-y

Ko RJ. Adulterants in Asian patent medicines. N Engl J Med 1998;339:847. DOI: https://doi.org/10.1056/NEJM199809173391214

Obi E, Akunyili DN, Ekpo B, Orisakwe OE. Heavy metal hazards of Nigerian herbal remedies. Sci Total Environ 2006;369:35-41. DOI: https://doi.org/10.1016/j.scitotenv.2006.04.024

Caldas ED, Machado LL. Cadmium, mercury and lead in medicinal herbs in Brazil. Food Chem Toxicol 2004;42:599-603. DOI: https://doi.org/10.1016/j.fct.2003.11.004

García-Rico L, Leyva-Perez J, Jara-Marini ME. Content and daily intake of copper, zinc, lead, cadmium, and mercury from dietary supplements in Mexico. Food Chem Toxicol 2007;45:1599-605. DOI: https://doi.org/10.1016/j.fct.2007.02.027

Ernst E. Adulteration of Chinese herbal medicines with synthetic drugs: A systematic review. J Intern Med 2002;252:107-13. DOI: https://doi.org/10.1046/j.1365-2796.2002.00999.x

Walker KM, Applequist WL. Adulteration of selected unprocessed botanicals in the US retail herbal trade. Econ Bot 2012;66:321-7. DOI: https://doi.org/10.1007/s12231-012-9211-6

Rathore KS. Standardisation and evaluation of herbal drug formulations. J Adv Lab Res Biol 2015; ???:???.

Posadzki P, Watson L, Ernst E. Contamination and adulteration of herbal medicinal products (HMPs): An overview of systematic reviews. Eur J Clin Pharmacol 2013;69:295-307. DOI: https://doi.org/10.1007/s00228-012-1353-z

Sagar BP, Zafar R, Panwar R, Kumar V, Mangla A. Herbal drugs standardization. Indian Pharm 2005;4:19-22.

Chen S, Yao H, Han J, Liu C, Song J, Shi L, et al. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One 2010;5:e8613. DOI: https://doi.org/10.1371/journal.pone.0008613

Singh B, Saxena AK, Chandan BK, Agarwal SG, Bhatia MS, Anand KK. Hepatoprotective effect of ethanolic extract of Eclipta alba on experimental liver damage in rats and mice. Phytother Res 1993;7:154-8. DOI: https://doi.org/10.1002/ptr.2650070212

Naik KS, Gurushanthaiah M, Kavimani M, Prabhu K, Lokanadham S. Hepatoprotective role of Eclipta alba against high fatty diet treated experimental models a histopathological study. Maedica 2018;13:217.

Ahirwar DK, Saxena RC. Hepatoprotective activity of ethanolic extract of Eclipta alba in albino rats. Biomed Pharmacol J 2008;1:235.

Kumar K, Katiyar AK, Swamy M, Sahni YP, Kumar S. Hepatoprotective effect of Eclipta alba on experimentally induced liver damage in rats. Indian J Vet Pathol 2013;37:159-63.

Indhuleka A, Jeyaraj M. Hepatoprotective effect of Eclipta alba on membrane marker enzymes against paracetamol induced liver damage. Orient J Chem 2019;35:1215. DOI: https://doi.org/10.13005/ojc/350343

Begum S, Lee MR, Gu LJ, Hossain M, Kim HK, Sung CK. Comparative hair restorer efficacy of medicinal herb on nude (Foxn) mice. Biomed Res Int 2014;14:1-9. DOI: https://doi.org/10.1155/2014/319795

Chaudhary H, Dhuna V, Singh J, Kamboj SS, Seshadri S. Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: An in vitro study. J Ethnopharmacol 2011;136:363-7. DOI: https://doi.org/10.1016/j.jep.2011.04.066

Gurrapu S, Mamidala E. In vitro antibacterial activity of alkaloids isolated from leaves of Eclipta alba against human pathogenic bacteria. Pharmacogn J 2017;9:573-7. DOI: https://doi.org/10.5530/pj.2017.4.91

Karthikumar S, Vigneswari K, Jegatheesan K. Screening of antibacterial and antioxidant activities of leaves of Eclipta prostrata (L). Sci Res Essay 2007;2:101-4.

Manvar D, Mishra M, Kumar S, Pandey VN. Identification and evaluation of anti hepatitis C virus phytochemicals from Eclipta alba. J Ethnopharmacol 2012;144:545-54. DOI: https://doi.org/10.1016/j.jep.2012.09.036

Banji O, Banji D, Annamalai AR, Manavalan R. Investigation on the effect of Eclipta alba on animal models of learning and memory. Indian J Physiol Pharmacol 2007;51:274.

Syed SD, Deepak M, Yogisha S, Chandrashekar AP, Muddarachappa KA, D’Souza P, et al. Trypsin inhibitory effect of wedelolactone and demethylwedelolactone. Phytother Res 2003;17:420-1. DOI: https://doi.org/10.1002/ptr.1153

Chanu TI, Roy SD, Chadha NK, Rawat KD, Sharma A, Kumar K. Antistress ability of Eclipta alba ethanol extract in Labeo calbasu fingerlings exposed to acid stress. Isr J Aquac Bamidegh 2013;65:1-9.