TOTAL PHENOLIC, FLAVONOID, TANNIN CONTENTS, AND ANTIOXIDANT ACTIVITY OF ORGANIC EXTRACTS OF VEGETATIVE AND FLOWERING STAGES OF MECARDONIA PROCUMBENS (MILL.) SWALL.

Authors

  • CHAITRA B NEGLUR Department of Botany, S. K Arts College and H. S. Kotambri Science Institute, Hubli, Karnataka, India
  • ASHWINI M GAJAKOSH Department of Botany, Govindram Seksaria Science College, Rani Channamma University, Belagavi, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i9.42039

Keywords:

Antioxidant property, Flowering stage, Mecardonia procumbens, Phenols, Tannins

Abstract

Objective: The objective of the study is to assess the total amount of phenols, flavonoid, and tannin content along with its antioxidant property of two physiological different stages such as vegetative and flowering in Mecardonia procumbens (Mill.) Swall.

Methods: subsequent extraction s done by socklet apparatus with solvents of increasing polarity: hexane, acetone, methanol, and to estimated for the biological metabolites.

Results: Stage of the plant significantly affected the quality of the phenols, flavonoids, and tannins. The highest amount of phenol (86.25±1.03 Gallic acid equivalents per gram of dry weight [GAE/g DW]) was reported in acetone extract in flowering sage similar result was with the flavanoid W(14.25±0.52 GAE/g DW). Whereas tannins were high in methanol extract of the flowering stage (49.52±1.02 mg catechin/g DW). The best scavenging activity was found in the flowering stage of acetone extract (3.5±0.06 μg/mL) and total antioxidant property was also in flowering stage of methanol extract (97.48 mg GAE/g DW) the M. procumbens.

Conclusion: Extracts of acetone and methanol were more effective and could be used as preservatives in food or pharmaceuticals.

Downloads

Download data is not yet available.

References

Mazid M, Khan TA, Mohammad F. Role of secondary metabolites in defense mechanisms of plants. Biol Med 2011;3:232-49.

Fratianni F, Tucci M, De-Palma M, Pepe R, Nazzaro F. Polyphenolic composition in different parts of some cultivars of globe artichoke (Cynara cardunculus L. var. Scolymus (L.) Fiori). Food Chem 2007;104:1282-6.

Tosun M, Celik F, Ercisli SO, Yilmaz S. Bioactive contents of commercial cultivars and local genotypes of walnut (Juglansregia L.). In: International Conference on Environmental and Agriculture Engineering. Vol. 15. Bangkok : Kasetsart University; 2011.

Dewanto X, Wu K, Adom K, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 2002;50:3010-4.

Sun B, Richardo-Da-Silvia JM, Spranger I. Critical factors of vanillin assay for catechins and proantho cyanidins. J Agric Food Chem 1998;46:4267-74.

Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root their relative astringency and radical scavenging effect. Chem Pharm Bull 1988;36:2090-7.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phospho-molybdenum complex: Specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-41.

Aisha M, Ahmed A, Fatma M, Abdelkhalek ME, Kalid AK. Morphologica and chemical characters of Petroselinum crispum (Mill) subjected to some biostimulants. Asian J Plant Sci 2018;17:96-106.

Mohammad M, Seyed N, Khaleghi M, Leila M. Total phenolic content and antioxidant activity of Fumaria vaillantii extract at three phenological stages assayed by various methods. Intern J Hort Sci Tech 2018;5:93-102.

Galvaz CJ, Martin-Cordero P, Houghton AM. Antioxidant activity of methanol extracts obtained from Plantago species. J Agric Food Chem 2005;53:1927-33.

Naczk M, Shahidi F. Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. J Pharm Biomed Anal 2006;41:1523-42.

Medini F, Fellah H, Ksouri R, Chedly A. Total phenolic, flavonoid and tannin contents and antioxidant andantimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. J Taibah Uni Sci 2014;8:216-24.

Khadhri A, Bouali I, Aouadhi C, Lagel MC, Masson E, Pizzi A. Determination of phenolic compounds by MALDI-TOF and essential oil composition by GC-MS during three development stages of Origanum majorana L. Biomed Chromatogr 2019;33:e4665.

Ichiho MK, Shiro M, Keijiro N, Yumico T, Minako Y, Tetsuo S, et al. Antioxidant capacity and polyphenols contents of extracts from crops cultivated in Japan and the effect of the cultivation. Environ Food Sci Technol Res 2013;19:69-79.

Naser JK, Layth SA, Ahmed AR, Joseph C. Chemical composition and antioxidant capacity of eggplant parts during vegetative and flowering stage. J Phys 2021;1294:092013.

Lacramioara O, Mihaela I, Marius NG, Maria MZ. Antioxidant activity of Plantago species in vegetative and flowering stage. Iran J Public Health 2015;4:142-4.

Sulas L, Re GA, Bullitta S. Chemical and productive propaerties of two Sarinian milk thistle (Silybum marianum (L.) Gaertn) population as sources of nutrients and antioxidants. Genet Resour Crop Evol 2016;63:315-26.

Spyridon A, Petropoulos A, Ângela F, Antoniadis V, Georgia N, Lillian B, et al. Chemical composition and antioxidant activity of Cichorium spinosum L. leaves in relation to developmental stage. Food Chem 2018;239:946-52.

Adegbaju OD, Otunola GA, Afolayan AJ. Effect of growth stage and season on the phytochemical content and antioxidant activities of crude extract of Celosia argentea L. Heliyon 2020;6:e04086.

Published

07-09-2021

How to Cite

CHAITRA B NEGLUR, and ASHWINI M GAJAKOSH. “) SWALL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 14, no. 9, Sept. 2021, pp. 84-86, doi:10.22159/ajpcr.2021.v14i9.42039.

Issue

Section

Original Article(s)