ANTIMOTOLITY EFFECT OF ORGANIC EXTRACTS OF ANTHEMIS MAURITIANA MAIRE & SENNEN FLOWERS ON THE RODENT ISOLATED JEJUNUM

Authors

  • Ahmed Karim
  • Hassan Zrouri
  • Souliman Amrani
  • Mohammed Bnouham
  • Abderrahim Ziyyat
  • Mohammed Aziz

Abstract

 

Objective: The aim of the present study was to investigate the effect of the organic extracts of Anthemis mauritiana (AM) Maire and Sennen (Asteraceae)
flowers on the contractile responses of the rabbit and rat jejunums and its possible mechanisms.
Methods: Soxhlet extraction with different successive solvents (petroleum ether, dichloromethane, ethyl acetate, and methanol) were prepared and
evaluated by preliminary phytochemical test. Antispasmodic effects of the extracts were evaluated against spontaneous contractions of rabbit and rat
jejunum contracted by carbachol and high K+ concentration.
Results: The phytochemical screening analysis revealed that the extracts of AM flowers contain flavonoids and tannins with variable quantities.
Petroleum ether and dichloromethane extracts showed the most relaxant effects in the rabbit jejunum at concentrations with IC50 value of 9.31±2.65 μg/ml
and 25.04±5.87 μg/ml respectively. Ethyl acetate showed a total inhibition at 1 mg/ml, but the methanol extract at 5 mg/ml. In contrast, the residual
aqueous extract was a reverse activity. And also all extracts caused an inhibitory effect on both K+ (75 mM) and CCh (10−6 M )induced contractions in
the rat jejunum except aqueous fraction. The extracts that have antispasmodic activity induced a marked depression on cumulative concentrationresponse
curve for carbachol and CaCl2.
Conclusions: The results indicate that the antispasmodic effect decreased with the polarity of the organic extracts, but the aqueous fraction had
a reverse effect. These results explain and confirm the popular use of genus of this plant for the treatment of gastrointestinal disturbances, and
encourage studies on new compounds, in order to obtain new antispasmodic agents.

Keywords: Anthemis mauritiana, Organic extract, Antispasmodic, Smooth muscle.

 

References

Bremer K. Asteraceae, Cladistics & Classification. Portland, Oregon: Timber Press; 1994. p. 435-78.

Oberprieler C. Phylogenetic relationships in Anthemis L. (Compositae, Anthemideae) based on nrDNA ITS sequence variation. Taxon 2001;50:745-62.

Bruno M, Bondi ML, Vassalo N, Gedris TE, Herz W. Guaianolides and other terpenoids from Anthemis aetnensis. Phytochemistry 1997;45:375-7.

Bruno M, Maggio A, Arnold NA, Díaz JG, Herz W. Sesquiterpene lactones from Anthemis plutonia. Phytochemistry 1998;49(6):1739‑740.

Bruno M, Rosselli S, Bondi ML, Gedris TE, Herz W. Sesquiterpene lactones of Anthemis alpestris. Biochem Syst Ecol 2002;30(9):891-5.

Vajs V, Bulatović V, Šavikin-Fodulović K, Menković N, Macura S, Juranić N, et al. Highly oxygenated guaianolides from Anthemis cretica subsp. Cretica. Phytochemistry 1999;50:287-91.

Vajs V, Todorovic N, Bulatovic VB, Menkovic N, Macura S, Juranic N, et al. Further sesquiterpene lactones from Anthemis carpatica. Phytochemistry 2000;54(6):625-33.

Harborne JB, Heywood VH, Saleh NA. Chemosystematics of the composiate: Flavonoid patterns in the chrysanthemum complex of the tribe Anthemideae. Phytochemistry 1970;9:2011-7.

Harborne JB, Baxter H, editors. The Handbook of Natural Flavonoids. Vol. 1. Chichester, UK: John Wiley and Sons Ltd.; 1999.

Harborne JB, Williams CA. Advances in flavonoid research since 1992. Phytochemistry 2000;55(6):481-504.

Williams CA, Greenham J, Harborne JB. The role of lipophilic and polar flavonoids in the classification of temperate members of the Anthemideae. Biochem Syst Ecol 2001;29(9):929-45.

Christensen L. Acetylenes and related compounds in Anthemidae. Phytochemistry 1992;31:7-49.

Bulatović VM, Menković NR, Vajs VE, Milosavljević SM, Äoković DD. The Essential oil from Anthemis carpatica. J Essent Oil Res 1997;9:397-400.

Bulatović VM, Menković NR, Vajs VE, Milosavljević SM, Äoković DD. The Essential oil of Anthemis montana. J Essent Oil Res 1998;10:223-6.

Uzel A, Guvensen A, Cetin E. Chemical composition and antimicrobial activity of the essential oils of Anthemis xylopoda O. Schwarz from Turkey. J Ethnopharmacol 2004;95(2-3):151-4.

Saroglou V, Dorizas N, Kypriotakis Z, Skaltsa HD. Analysis of the essential oil composition of eight Anthemis species from Greece. J Chromatogr A 2006;1104(1-2):313-22.

Melegari M, Albasini A, Pecorari P, Vampa G, Rinaldi M, Rossi T, et al. Chemical characteristics and pharmacological properties of the essential oils of Anthemis nobilis. Fitoterapia 1988;59:445-9.

Rossi T, Melegari M, Bianchi A, Albasini A, Vampa G. Sedative, anti-inflammatory and anti-diuretic effects induced in rats by essential oils of varieties of Anthemis nobilis: A comparative study. Pharmacol Res Commun 1988;20 Suppl 5:71-4.

Karim A, Berrabah M, Mekhfi H, Ziyyat A, Legssyer A, Bouali A, et al. Effect of essential oil of Anthemis mauritiana Maire & Sennen flowers on intestinal smooth muscle contractility. J Smooth Muscle Res 2010;46(1):65-75.

Aziz M, Karim A, El Ouariachi el M, Bouyanzer A, Amrani S,Mekhfi H, et al. Relaxant effect of essential oil of Artemisia herba-alba asso. on rodent jejunum contractions. Sci Pharm 2012;80(2):457-67.

Hagerman A, Harvey-Mueller I, Makkar HP. Quantification of Tannins in Tree Foliage-A Laboratory Manual. Vienna: FAO/IAEA; 2000. p. 4-7.

Hagerman AE, Butler LG. Protein precipitation method for the quantitative determination of tannins. J Agric Food Chem 1978;26:809‑12.

Jay M, Gonnet JF, Wollenweber E, Voirin B. Sur l’analyse qualitative des aglycones flavoniques dans une optique chimiotaxonomique. Phytochemistry 1975;14:1605-12.

Karaki H, Weiss GB. Calcium release in smooth muscle. Life Sci 1988;42(2):111-22.

Bolton TB. Mechanisms of action of transmitters and other substances on smooth muscle. Physiol Rev 1979;59(3):606-718.

Bolton TB, Prestwich SA, Zholos AV, Gordienko DV. Excitation-contraction coupling in gastrointestinal and other smooth muscles. Annu Rev Physiol 1999;61:85-115.

Goyal RK. Identification, localization and classification of muscarinic receptor subtypes in the gut. Life Sci 1988;43(26):2209-20.

Boegge SC, Kesper S, Verspohl EJ, Nahrstedt A. Reduction of ACh-induced contraction of rat isolated ileum by coptisine, (+)-caffeoylmalic acid, Chelidonium majus, and Corydalis lutea extracts. Planta Med 1996;62(2):173-4.

Pietta P. Flavonoids in medicinal plants. In: Rive-Evans CV, Packer L, editors. Flavonoids in Health and Disease. New York: Marcel Dekker; 1998. p. 61-110.

Heinrich M, Rimpler H, Barrera NA. Indigenous phytotherapy of gastrointestinal disorders in a low and mix community (Oaxaca, Mexico): Ethnopharmacologic evaluation. J Ethnopharmacol 1992;36:63-80.

Published

2015-03-01

How to Cite

Karim, A., H. Zrouri, S. Amrani, M. Bnouham, A. Ziyyat, and M. Aziz. “ANTIMOTOLITY EFFECT OF ORGANIC EXTRACTS OF ANTHEMIS MAURITIANA MAIRE & SENNEN FLOWERS ON THE RODENT ISOLATED JEJUNUM”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 2, Mar. 2015, pp. 154-8, https://innovareacademics.in/journals/index.php/ajpcr/article/view/4218.

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