• Dipti Kaspate AISSMS COLLEGE OF PHARMACY, PUNE-411001.
  • Tina Saldanha
  • Swati Narkhede
  • Bhagyarshree Karmarkar
  • Karan Shah


The objective of this study to investigate the acute oral toxicity effects of Sinapis alba seeds in swiss albino female mice based on OECD guideline 423.
The ethanolic extract was administered orally at two different doses of 300 mg/kg and 2000 mg/kg of body weight. The female mice were observed
continuously for 4 hrs for behavioural and autonomic profiles and for any other sign of toxicity or mortality up to a period of 14 days. In between
14 days observation; body weight, feed, and water intake were also observed on 1st, 7th and 14th day. At the end of 14 day's observation, female mice
from each group were sacrificed by cervical dislocation and vital organs were removed, weighed and made histological sections. All results were
shown that there were no any toxic effects, feed and water intake were maximum on 7th day. Body weight, organ weight and histological sections were
also normal.
Keywords: Acute oral toxicity study, Sinapis alba seeds ethanolic extract, Feed and water intake, Body weight, Vital organ weight, Histology.


Author Biography





Dipalma JR. Drills Pharmacology Medicine. 4th ed. London: McGraw Hill Book Ltd.; 1971. p. 21-43.

Farnsworth NR, Akerele O, Bingel AS, Soejarto DD, Guo Z. Medicinal plants in therapy. Bull World Health Organ 1985;63(6):965-81.

Padua LS, Bunyaprapkatsara N, Lemmens RH. Medicinal and Poisonous Plants. Leiden: Blachuys Publisher; 1999. p. 711.

Jamaran I. The role of science and technology on the development of medicinal plants agro industry. Proceeding of Coordination and Strategic Consultation on the Development of Medicinal Plant Agroindustry, Bogor: Bogor Agriculture University; 1995. p. 233.

Balke D. Rapid aqueous extraction of mucilage from whole white mustard seed. Food Res Int 2000;33 Suppl 5:347-56.

Adams M, Schneider SV, Kluge M, Kessler M, Hamburger M. Epilepsy in the Renaissance: A survey of remedies from 16th and 17th century German herbals. J Ethnopharmacol 2012;143(1):1-13.

Nadkarni KR. Indian Materia Medica. 3rd ed. Mumbai: Bombay Popular Prakashan; 1954.

Sujatha, Ravishankar, Mariajancyrani, Chandramohan. Preliminary phytochemical investigation and antimicrobial activity of Sinapis Alba. Scholars J Appl Med Sci 2013;1 Suppl 3:138-41.

Sahjapal B. Kasyapa Samhita. 7th ed. Varanasi: Chaukhambha Sanskrit Santhan; 2000.

Seth SD, Sharma B. Medicinal plants in India. Indian J Med Res 2004;120(1):9-11.

Bent S, Ko R. Commonly used herbal medicines in the United States: A review. Am J Med 2004;116(7):478-85.

Malathi R, Gomaz P. Evaluation of preliminary toxicity studies on the methanolic leave extract of Tylophora asthmatica in experimental rats. J Pharmacol Toxicol 2008;3:34-40.

Sahgal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S, Mansor SM. Brine shrimp lethality and acute oral toxicity studies on Swietenia mahagoni (Linn.) Jacq. seed methanolic extract. Pharmacognosy Res 2010;2(4):215-20.

Chakravarthy BK. Herbal medicine safety and efficacy guidelines. Regul Aff J 1993;4:699-01.

Deng JF, Lin TJ, Kao WF, Chen SS. The difficulty in handling poisonings associated with Chinese traditional medicine: A poison control center experience for 1991-1993. Vet Hum Toxicol 1997;39(2):106-14.

Deng JF. Clinical and laboratory investigations in herbal poisonings. Toxicology 2002;181-182:571-6.

Riaz A, Khan RA, Ahmed S, Afroz S. Assessment of acute toxicity and reproductive capability of a herbal combination. Pak J Pharm Sci 2010;23(3):291-4.

Chan K. Some aspects of toxic contaminants in herbal medicines. Chemosphere 2003;52(9):1361-71.

Dyson A. Discovering indigenous healing plants of the herb and fragrance gardens at Kirstenbosch National Botanical Garden. National Botanical Institute. Cape Town: Printing Press; 1998.

Sangeetha G, Sekar Babu M, Balammal G, Mohan Krishna L. Toxicological studies on ethanolic extract of Garcinea indica Chois in experimental animals. Int J Pharm Biol 2011;1 Suppl 1:15-9.

Reddy UB. Acute and sub acute toxicity of amalakyadi churna. Pharmacologyonline 2010;1:625-33.



How to Cite

Kaspate, D., T. Saldanha, S. Narkhede, B. Karmarkar, and K. Shah. “ACUTE ORAL TOXICITY OF SINAPIS ALBA IN SWISS ALBINO MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 2, Mar. 2015, pp. 182-4,



Original Article(s)