SCREENING OF NEROLI OIL ON PARACETAMOL INDUCED HEPATOTOXICITY IN RATS
Keywords:Neroli oil, Paracetamol, Silymarin, Hepatotoxicity, Histopathology
Objective: The objective of the study was to evaluate the hepatoprotective effect of Neroli oil against Paracetamol induced liver injury in Wistar rats.
Methods: Changes in the serum biomarker enzymes, namely, alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), direct bilirubin, total bilirubin, total cholesterol, and albumin were estimated. Oxidative stress parameter such as MDA, SOD, GSH, and catalase levels was also estimated along with the liver weight and volume.
Results: Neroli oil showed significant reduction in all elevated biochemical parameters (namely, ALT, AST, ALP, direct bilirubin, total bilirubin, and total cholesterol), and significant increase in reduced level of albumin. Oil showed significant increase in GSH and catalase level and decreased in LPO level. Histopathological determinations confirmed biochemical findings.
Conclusion: Neroli oil showed a significant hepatoprotective effect against Paracetamol induced hepatic damage as depicted by its protective activity on functional, physical, biochemical, antioxidant, and histological changes in liver.
Banothu B, Kusuma P, Alli R, Gorre T, Rani GS. Hepatoprotective studies of Mussaenda raiateensis extracts on carbon tetrachloride induced hepatotoxicity in rats. Int J Pharm Biol 2011;1:25-9.
Kanchana N, Sadiq AM. Hepatoprotective effect of Plumbago zeylanica on paracetamol induced liver toxicity in rats. Int J Pharm Pharm Sci 2011;3:152-4.
El-Reddy MS, Badawi AA, Safar MM, Mohsan AM. Noisome as a novel pharmaceutical formulation encapsulating the hepatoprotective drug silymarin. Int J Pharm Pharm Sci 2012;4:549-59.
Ines E, Hajer D, Rachid C. Aromatic quality of Tunisian Sour Orange essential oils: Comparison between traditional and industrial extraction. Nat Volatiles Essent Oils 2014;1:66-72.
Chen YJ, Shih Y, Chang TM, Wang MF, Lan SS, Cheng FC. Inhalation of Neroli essential oil and its anxiolytic effects in animals. In: Proceedings of the Measuring Behavior. ???: ???; 2008. p. 256-7.
David Gubb Compliance and Reg. Neroli Oil Phase. Material Safety Data Sheet. Revised Document; 2008.
Noorani AA, Kale MK. Pre-treatment of albino rats with methanolic fruit extract of Randia dumetorum (L.) protects against alcohol induced liver damage. Korean J Physiol Pharmacol 2012;16:125-30.
Kit S (A). (Modified IFCC Method). Goa: Crest Biosystems; 2011.
SGPT. (ALAT) KIT (Modified IFCC Method). Goa: Crest Biosystems; 2011.
Alkaline Phosphatase (ALP) KIT. (Adaptation by Wilkinson et al. of the Bessey, Lowry et al. Method). Germany: Erba Diagnostics Mannheim GmbH; 2011.
KIT A (A). (BCG Dye Method). Germany: Erba Diagnostics Mannheim GmbH; 2012.
Cholesterol Kit. (Modified Roeschlau’s Method). Germany: Erba diagnostics Mannheim GmbH; 2011.
Bilirubin (BIT and BID) KIT. (Diazo Method). Germany: Erba Diagnostics Mannheim GmbH; 2012.
Fraga CG, Leibovitz BE, Tappel AL. Lipid peroxidation measured as TBARS in tissue slices: Characterization and comparison with homogenates and microsomes. Free Radic Biol Med 1988;4:155-61. doi: 10.1016 / 0891-5849(88)90023-8, PMID 3356355
Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94. doi: 10.1016 / 0003-2697(72)90132-7, PMID 4556490
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7. doi: 10.1016 / 0003-9861(59)90090-6, PMID 13650640
Nandi A, Chatterjee IB. Scavenging of superoxide radical by ascorbic acid. J Biosci 1987;11:435-41. doi: 10.1007/BF02704692
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