HISTOGENESIS OF HUMAN FETAL STOMACH AT VARIOUS GESTATIONAL AGES – AN OBSERVATIONAL STUDY
Keywords:Chief cells, Histogenesis, Mucus neck cells, Zymogen Cells
Objective: Knowledge on timelines of structural and histological development of fetal stomach helps in the diagnosis of abnormalities (duplication cyst of the pylorus, heterotopic pancreatic tissue, etc.,) and weighing interventions as an option. The present study was undertaken to establish the timelines of the developmental process of stomach (e.g., appearance of curvatures, gastric pit, gastric glands, and various cell types) correlating with the gestational age.
Methods: Fifty normal, aborted and unclaimed fetuses were obtained from local private and government hospitals observing all formalities. The tissues of 3–5 mm thickness were taken and preserved in 10% formalin for 72 h, followed by tissue processing and staining with hematoxylin eosin and periodic acid Schiff and observations were made under microscope.
Results: Lesser and greater curvatures appeared in the first trimester. All the four layers and gastric pits were observed at 16th week, cuboidal epithelium turns columnar and nidus of gastric glands, circular muscular layer submucuosa noted by 18th week, longitudinal layer of muscle appeared at 20th week, parietal, zymogenand mucus neck cells observed by 24th week in fundus, and body oblique muscle was evident by 28th week and near adult pattern seen by 32nd week of gestation.
Conclusion: From the available literature, very few studies have reported regarding histogenesis of human fetal stomach in India. The present study is one such to supplement the known data and knowledge regarding histogenesis of human fetal stomach and help in diagnosis and treatment of related congenital anomalies.
Williams PL, Bannister LH, Berry MM, Collins P, Dyson M, Dusseck JE, et al. Gray’s Anatomy: The Anatomical Basis of Medicine and Surgery. 38th ed. London, United Kingdom: ELBS, Churchill Livingstone; 1995. p. 183-6.
Arey LB. Developmental Anatomy. 7th ed. Philadelphia: WB Saunders; 1974.
Grand RJ, Watkins JB, Torti FM. Development of the human gastrointestinal tract-a review. Gastroenterology 1976;70:790-810.
Moore KL, Persaud TV. The Developing Human Clinically Oriented Embryology. 5th ed. Philadelphia, PA: WB Saunders Company; 1993. p. 237-48.
Sharples W. The histogenesis of the argentaffin cells in the stomach and duodenum of the rat. The biological laboratories Radcliffe college, Cambridge, Massachusctts. Anat Rec 1954;91:107-241.
Aitchison M, brown IL. Intrinsic factor in the human fetal stomach immunocytochemical study. J Anat 1998;160:211-7.
Montella A, Dessi AL. The gastric mucosa of the human fetus. II. Ontogenesis of the gastric glands in the strict sense. Boll Soc Ital Biol Sper 1991;67:279-86.
Otani H, Yoneyama T, Hashimoto R, Hatta T, Tanaka O. Ultra structure of the developing stomach in human embryos. Anat Embryol (Berl) 1992;187:145-51.
Chénard M, Basque JR, Chailler P, Tremblay E, Beaulieu JF, Ménard D. Expression of integrin subunits correlates with differentiation of epithelial cell lineages in developing human gastric mucosa. Anat Embryol (Berl) 2000;202:223-33. doi: 10.1007/s004290000104, PMID 10994995
Jit I. The development of the muscular coats in the human oesophagus, stomach and small intestine. J Anat Soc India 1956;5:1-13.
Singh I. A note on entrochromoffin cells in islets of ectopic intestinal mucosa in the human stomach. J Anat Soc India 1962;11:57-9.
Chimmalgi M, Sant SM. Study of fetal stomach under light microscope. J Anat Soc India 2005;54:1-9.
Hamilton WJ, Mossman HW, Boyd JD. Human Embryology: Prenatal Development of Form and Function. 4th ed., Vol. 175. United States: Williams and Wilkins Co; 1978. p. 291-377.
Ménard D, Arsenault P. Cell proliferation in developing human stomach. Anat Embryol (Berl) 1990;182:509-16. doi: 10.1007/BF00178918, PMID 2291496
Montella A, Dessi AL. The gastric mucosa of the human fetus. I. The surface epithelium. Boll Soc Ital Biol Sper 1990;66:315-22.
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