AN ASSESSMENT OF THE MICROBIOLOGICAL QUALITY OF ANTI-VIRAL CHURNA SOLD IN INDIA BY AYURVEDIC MEDICAL PRACTITIONERS
Keywords:Herbal formulation, Viral disease, Microbial load
Objectives: The objectives behind of this study are to analyze the unregistered herbal products dispensed by Ayurvedic practitioners, as per the WHO guidelines for determination of potentially hazardous contaminants. To aware the registered medical practitioners regarding contamination of herbal product, the patients are using unregistered herbal products from registered medical practitioners.
Methods: The method employed in this study was a pour plate method. The specific media plates were incubated for 24 h at 37°C. On a colony counter, the plate was placed and observed for the presence of micro-organisms. The media used were nutrient agar medium and cetrimide nutrients, agar, saline agar, and MacConkey agar.
Result: The present study found microbial contamination in herbal products that are widely available across the country. The formulations coded AV5, AV6, and AV7 were found to be contaminated by Pseudomonas aeruginosa and AV5 and AV6 were found to be contaminated by Escherichia coli, whereas the formulations coded AV1 and AV2 were found to be contaminated by Staphylococcus aureus in above permissible limit, posing a risk of infection if consumed.
Conclusion: According to the findings of this study, it shows that few herbal formulations were showing the presence of micro-organism above the permissible of given by the WHO.
Choi DW, Kim JH, Cho SY, Kim DH, Chang SY. Regulation and quality control of herbal drugs in Korea. Toxicology 2002;181-182:581-6. doi: 10.1016/s0300-483x(02)00487-0, PMID 12505370
Khanyile ZC, Singh N, Smith M, Shode FO, Mngomezulu S, Dewir YH. Camparative assesment of Bacterial contamination in commercial herbal product of Lessertia futescens. Am Eur J Agric Environ Sci 2009;5:494-9.
Dubey NK, Kumar A, Singh P, Shukla R. Microbial contamination of raw materials: A major reason for the decline of India’s share in the global herbal market. Curr Sci 2008;95:25.
Chan K. Some aspects of toxic contaminants in herbal medicines. Chemosphere 2003;52:1361-71. doi: 10.1016/S0045-6535(03)00471-5, PMID 12867165
Available from: https://www.researchgate.net/publication/320306420_ Quality_Control_of_Herbal_Drugs
Vestbo J, Hurd SS, Agustí AG, Jones PW, Vogelmeier C, Anzueto A, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: Gold executive summary. Am J Respir Crit Care Med 2013;187:347-65. doi: 10.1164/rccm.201204- 0596PP, PMID 22878278
Mathers CD, Loncar D. Projections of global mortality and burden of disease from 2002 to 2030. PLoS Med 2006;3:e442. doi: 10.1371/ journal.pmed.0030442, PMID 17132052
Sethi S. Bacterial infection and the pathogenesis of COPD. Chest 2000;117;286S-91. doi: 10.1378/chest.117.5_suppl_1.286s, PMID 10843957
Mallia P, Johnston SL. How viral infections cause exacerbation of airway diseases. Chest 2006;130:1203-10.
Proud D, Chow CW. Role of viral infections in asthma and chronic obstructive pulmonary disease. Am J Respir Cell Mol Biol 2006;35:513-8. doi: 10.1165/rcmb.2006-0199TR, PMID 16778148
Tan WC, Xiang X, Qiu D, Ng TP, Lam SF, Hegele RG. Epidemiology of respiratory viruses in patients hospitalized with near-fatal asthma, acute exacerbations of asthma, or chronic obstructive pulmonary disease. Am J Med 2003;115:272-7. doi: 10.1016/s0002-9343(03)00353-x, PMID 12967691
Mallia P, Contoli M, Caramori G, Pandit A, Johnston SL, Papi A. Exacerbations of asthma and chronic obstructive pulmonary disease (COPD): Focus on virus induced exacerbations. Curr Pharm Des 2007;13:73-97. doi: 10.2174/138161207779313777, PMID 17266589
Ko FW, Ip M, Chan PK, Chan MC, To KW, Ng SS, et al. Viral etiology of acute Exacerbations of COPD in Hong Kong. Chest 2007;132:900-8. doi: 10.1378/chest.07-0530, PMID 17573516
Martinello RA, Esper F, Weibel C, Ferguson D, Landry ML, Kahn JS. Human Metapneu-movirus and exacerbations of chronic obstructive pulmonary disease. J Infect 2006;53:248-54. doi: 10.1016/j. jinf.2005.11.010, PMID 16412516
Mukharji PK, Rai S, Bhattacharya S, Wahile A, Pada Saha B. Marker analysis of poly herbal formulation Triphala-A well-known Indian traditional medicine. Indian J Trad Knowl 2008;7:379-83.
Okunlola A, Adewoyin BA, Odeku OA. Evaluation of pharmaceutical and microbial qualities of some herbal medicinal products in South Western Nigeria. Trop J Pharm Res 2007;6:661-70. doi: 10.4314/tjpr. v6i1.14644
Waterman RF, Sumner ED, Baldwin JN, Warren FW. Survival of Staphylococcus aureus on pharmaceutical oral solid dosage forms. J Pharm Sci 1973;62:1317-20. doi: 10.1002/jps.2600620818, PMID 4725177
Vandoorne H, Claushuis EP. The quantitative determination of Enterobacteriaceae in pharmaceutical preparations. Int J Pharm 1979;4:119-25. doi: 10.1016/0378-5173(79)90059-0
Abba D, Inabo HI, Yakubu SE, Olonitola OS. Contamination of herbal medicinal products marketed in Kaduna metropolis with selected pathogenic bacteria. Afr J Tradit Complement Altern Med 2009;6:70-7.
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