ENZYME PRODUCTION ABILITY BY BACILLUS SUBTILIS AND BACILLUS LICHENIFORMIS - A COMPARATIVE STUDY

Authors

  • S Vijayalakshmi
  • J Ranjitha
  • V Devi Rajeswari

Abstract

Objective: B.subtilis and B.licheniformis have been reported as one of the potential protease producer. Hence, an attempt has been made to optimize and compare the enzyme production by entrapment and fermentor conditions. Methods: Growth profile of both the strains has been studied. Optimization of culture conditions with respect to pH, temperature, agitation, carbon, nitrogen sources and metals has also been analyzed. Immobilization studies have been conducted for the strains. Enzyme production ability in fermentor has been premeditated. Results: Growth profile for both the strains was found to be similar. Strains were optimized for maximal enzyme production. Fermentation has yielded higher production of enzyme (8080 U/ml) compared to immobilization (850 U/ml). Conclusion: B.subtilis was more stable in enzyme production with immobilization and fermentation when compared to B.licheniformis.

Keywords: B.subtilis, B.licheniformis, Immobilization, Fermentation

References

Shakila Begam M, Stanly Pradeep, F, Pradeep BV. Production, Purification, Characterization and applications of Lipase from Serratia marcescens MBB05, Asian. J. Pharm. Clin. Res, 2012, 5, 237-245.

Ahmed SA, Saleh SA, Abdel-Fattah AF. Stabilization of Bacillus Licheniformis ATCC 21415 alkaline protease by immobilization and modification, Aus. J. Bas. Appl. Sci, 2007, 1, 313-322.

Beg QK, Gupta R, Lorenz P. Bacterial Alkaline Proteases. Molecular Approaches and Industrial Applications, Appl. Microb. Biotechnol, 2002, 59, 15-32.

Longo, MA, Novella IS, Garcia L, Diaz M. Diffusion of proteases in calcium alginate beads. Enzym. Microb. Technol, 1992, 14, 586-590.

Kumar R, Vats R. Protease production by Bacillus subtilis Immobilized on different matrices, New. York. Sci. J, 2010, 3, 20-24.

Prakasham RS, Subba Rao CH, Sharma PN, Green. Gram husk-an inexpensive substrate for alkaline protease production by Bacillus sp. in solid-state fermentation, Bioresour. Technol, 2006, 97, 1449-1454.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein Measurement with with the folin phenol reagent, J. Biol. Chem, 1951, 193, 265–73.

Singh J, Vohra RM, Sahoo DK. Enhanced production of alkaline proteases by Bacillus sphaericus using fed-batch culture, Proces. Biochem, 2004, 39, 1093-1101.

Rahman RNZA, Geok LP, Basri M, Salleh AB. Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K, Bioresour. Technol, 2005, 96, 429–436.

Rao YK, Lu SC, Liu BL, Tzeng YM. Enhanced production of an extracellular protease from Beauveria bassiana by optimization of cultivation processes, J. Biochem. Eng, 2006, 28, 57-66.

Joo HS, Kumar CG, Park GC, Paik SR, Chang CS. Oxidant and SDS-stable alkaline protease from Bacillus clausii-52: production and some properties, J. Appl. Microb, 2003, 95, 267-272 .

Manni L, Jellouli K, Agrebi R, Bayoudh A, Nasri M. Biochemical and molecular characterization of a novel calcium-dependent metalloprotease from Bacillus cereus SV1, Proces. Biochem, 2008, 43, 522–530.

Adinarayana K, Bapi Raju KVVSN, Ellaiah P. Investigation on alkaline protease production with B. subtilis PE-11 immobilized in calcium alginate gel beads, Proces. Biochem, 2004, 39, 1331-1339.

Francisco JU, Adriana L, Luis A, Garcia MD. Fermentation conditions increasing protease production by Serratia marcescens in fresh whey, Rev. Tec. Ing. Univ. Zulia, 2008, 31, 79-89.

Khosravi-Darani K, Falahatpishe HR, Jalali M. Alkaline protease production on date waste by an alkalophilic Bacillus sp. 2-5 isolated from soil, Afr. J. Biotechnol, 2008, 7, 1536-1542.

Kumar CG, Takagi H. Research review paper: microbial alkaline proteases: from a bio-industrial viewpoint, Biotechnol. Adv, 1999, 17, 561-94.

Mussarat Shaheen B, Aamer Ali Shah, Abdul Hameed, Fariha Hasan. Influence of Culture Conditions on Production and Activity of Protease from Bacillus Subtilis, Pak. J. Bot, 2008, 40, 2161-2169.

Potumarthi R, Subhakar Ch, Annapurna Jetty. Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheniformis NCIM-2042: Effect of aeration and agitation regimes, Biochem. Eng. J, 2007, 34, 185-192.

Genckal H, Tari C. Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats, Enzym. Microb. Technol, 2006, 39, 703-710.

Mukherjee AK, Adhikari H, Rai SK. Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: Characterization and application of enzyme in detergent formulation, Biochem. Eng. J, 2008, 39, 353-361.

Sen S, Satyanarayana T. Optimization of alkaline protease production by thermophilic Bacillus licheniformis S-40, Ind. J. Microbiol, 1993, 33, 43-47.

Abidi F, Limam F, Marzouki MN. Purification and characterization of an alkaline protease Prot 1 from Botrytis cinerea. Assay as biodetergent catalyst, Appl. Biochem. Biotechnol, 2007, 141, 361-76.

Sangeeta Negi, Suneel Gupta, Rintu Banerjee. Extraction and Purification of Glucoamylase and Protease produced by Aspergillus awamori in a Single-Stage Fermentation, Food. Technol. Biotechnol, 2011, 49, 310-315.

Published

2013-10-01

How to Cite

Vijayalakshmi, S., J. . Ranjitha, and V. D. Rajeswari. “ENZYME PRODUCTION ABILITY BY BACILLUS SUBTILIS AND BACILLUS LICHENIFORMIS - A COMPARATIVE STUDY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 4, Oct. 2013, pp. 29-32, https://innovareacademics.in/journals/index.php/ajpcr/article/view/479.

Issue

Section

Articles