ANTIBIOTIC RESISTANCE PROFILE OF BACTERIAL PATHOGENS IN THE GUT OF P. AMERICANA

Authors

  • Shini Zacharia
  • Asha Peter
  • Jyothis Mathew

Abstract

The study was conducted to determine the antibiotic sensitivity of various bacterial isolates including L. monocytogenes obtained from the intestinal content of P. americana captured from hospitals, domestic environments, restaurants and market places. The antimicrobial susceptibility of the bacterial isolates was determined by Kirby-Bauer disk diffusion method. Among the different groups of antibiotics, cephalosporins resistance was obvious in all the bacterial isolates under study. E. faecium, the most predominant isolate in the study, showed noticeable resistance to penicillin (39percent), erythromycin (35percent) and cloxacillin (32percent) apart from its cephalosporin resistance. Among the Gram negative isolates, though resistance to quinolones was not as apparent as cephalosprins, tendency to resist nalidixic acid was evident particularly in P. aeruginosa (79percent). Resistance to penicillin, nalidixic acid was noticed in all the Listeria species under study. The multidrug resistant bacteria carried by the omnipresent insect cockroach in their intestine as noticed in this study urges the necessity of further epidemiological studies for revealing the role of this insect in nosocomial infection and food spoilage.

Key words

P. americana, bacterial pathogens, antibiotic sensitivity

References

Pechal JL, Austin J, Gold R, Tomberlin JK. Epidemiology and spatial relationships of bacteria associated with Periplaneta americana (Blattodea: Blattidae) in central Texas. Agric Urban Entomol. 2007; 24: 205–216.

Cotton MF, Wasserman E, Pieper CH, Theron DC, van Tubbergh D, Campbell G, Fang FC, Barnes J. Invasive disease due to extended spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: the possible role of cockroaches. J Hosp Infect. 2000; 44:13-7.

Lemmen SW, Ha¨fner H, Zolldann D, Stanzel S, Lu¨tticken R. Distribution of multi-resistant Gram-negative versus Gram-positive bacteria in the hospital inanimate environment. J Hosp Infect. 2004; 56 (3): 191–197.

Bauer AW, Kirby WM, Sherris J C, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45:493-6.

National committee for clinical laboratory standards NCCLS. Performance standards for antimicrobial susceptibility testing; Eleventh informational supplement M100-S11. 2001; Wayne, PA.

Silbergeld EK, Davis M, Leibler JH, Peterson AE. One Reservoir: Redefining the community origins of antimicrobial - resistant infections. Med Clin N Am. 2008; 92: 1391–1407.

Forbes BA, Sahm DF, Weissfel, AS. Bailey and Scott’s Diagnostic Microbiology, 10th edn. 1998; Mosby Inc., St. Louis Missouri, U. S. A.

Karmarkar MG, Gershom ES, Mehta PR. Enterococcal infections with special reference to phenotypic characterization & drug resistance. Indian J Med Res. 2004;119: 22-25.

Fotedar R, Shriniwas UB, Verma A.. Cockroaches (Blattella germanica) as carriers of microorganisms of medical importance in hospitals. Epidemiol Infect. 1991;107: 181–187.

Tilahun B, Worku B, Tachbele E, Terefe S, Kloos H, Legesse W. High load of multi-drug resistant nosocomial neonatal pathogens carried by cockroaches in a neonatal intensive care unit at Tikur Anbessa specialized hospital, Addis Ababa, Ethiopia. Antimicrob Resist Infect Control. 2012; 1:12.

Moland ES, Sanders CC, Thomson KS. Can results obtained with commercially available microscan microdilution panels serve as an indicator of β-Lactamase production among Escherichia coli and Klebsiella Isolates with hidden resistance to expandedspectrum cephalosporins and aztreonam? J Clin Microbiol. 1998; 36: 2575–2579.

Sirot D, Sirot J, Labia R, Morand A,Courvalin P, Darfeuille-Michaud A, Perroux R, Cluzel R. Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel β-lactamase. J Antimicrob Chemother. 1987; 20 : 323-334.

Subha A, Ananthan S. Extended spectrum beta lactamase (ESBL) mediated resistance to third generation cephalosporins among klebsiella pneumoniae in Chennai. Indian J Med Microbiol. 2002; 20:92-5.

Rasool SA, Ahmad A, Khan S, Wahab A. Plasmid borne antibiotic resistance factors among indigenous Klebsiella. Pak J Bot. 2003; 35: 243–248.

Pagani L, Migliavacca R, Pallecchi L, Matti C, Giacobone E, Amicosante G, Romero E, Rossolini GM. Emerging Extended-Spectrum β-Lactamases in Proteus mirabilis. J Clin Microbiol. 2002; 40: 1549–1552.

O'Hara CM, Brenner FW, Miller JM. Classification, identification, and clinical significance of Proteus, Providencia and Morganella. Clin Microbiol Rev. 2000;13: 534–546.

Carmeli Y, Troillet N, George M, Eliopoulos GM, Samore MH. Emergence of antibiotic-resistant Pseudomonas aeruginosa: Comparison of risks associated with different antipseudomonal agents. Antimicrob. Agents Chemother. 1999; 43 : 1379-1382.

Muller-Premru M, Gubina M. Serotype, antimicrobial susceptibility and clone distribution of Pseudomonas aeruginosa in a University hospital. Zentralbl Bakteriol. 2000 ; 289: 857-867.

Mehta M, Punia JN, Joshi RM. Antibiotic resistance in pseudomonas aeruginosa strains isolated from various clinical specimens - A retrospective study. Indian J Med Microbiol. 2001; 19:232.

Fink MP, Snydman DR, Niederman MS, Leeper KV, Johnson RH, Heard SO, Wunderink R G, Caldwell JW, Schentag JJ, Siami JA, Zameck RL, Haverstock DC, Reinhart HH, Echols RM. Treatment of severe pneumonia in hospitalized patients: results of a multicenter, randomized, double-blind trial comparing intravenous ciprofloxacin with imipenem-cilastin. Antimicrob Agents Chemother. 1994; 38(3):547–557.

Payne DJ, Aymes SG. Transferable resistance to extended-spectrum beta-lactams: a major threat or minor inconvenience? J Antimicrob Chemother. 1991; 27(3):255-261.

John JF, Harvin AM. History and evolution of antibiotic resistance in coagulase-negative staphylococci: susceptibility profiles of new anti-staphylococcal agents. Ther Clin Risk Manag 2007 ; 3:1143–52.

Irlinger F. Safety assessment of dairy microorganisms: Coagulase-negative staphylococci. Int. J Food Microbiol. 2008; 126:302–10.

Prazak MA, Murano EA, Mercado I, Acuff GR. Antimicrobial resistance of Listeria monocytogenes isolated from various cabbage farms and packing sheds in Texas. J Food Prot. 2002; 65:1796-1799.

Srinivasan V, Nam HM, Nguyen LT, Tamilselvam B, Murinda SE, Oliver SP. Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathog Dis. 2005; 2:201-211.

Troxler R, Von Graevenitz A, Funke G, Wiedemann B, Stock I. Natural antibiotic susceptibility of Listeria species: L. grayi, L. innocua, L. ivanovii, L. monocytogenes, L. seeligeri and L. welshimeri strains. Clin Microbiol Infect. 2000; 6:525.

Reis CM, Barbosa AV, Rusak LA, Vallim DC, Hofer E. Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil. Rev Soc Bras Med Trop. 2011; 44:173-6.

Poyart-Salmeron C, Trieu-Cuot P, Carlier C, MacGowan A, McLauchlin J, Courvalin P. Genetic basis of tetracycline resistance in clinical isolates of Listeria monocytogenes. Antimicrob Agents Chemother. 1992; 36:463-466.

Charpentier E, Gerbaud G, Rocourt J, Courvalin P. Incidence of antibiotic resistance in Listeria species. J Infect Dis. 1995; 172:277-81.

Published

2013-10-01

How to Cite

Zacharia, S., A. Peter, and J. Mathew. “ANTIBIOTIC RESISTANCE PROFILE OF BACTERIAL PATHOGENS IN THE GUT OF P. AMERICANA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 4, Oct. 2013, pp. 42-46, https://innovareacademics.in/journals/index.php/ajpcr/article/view/482.

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