INHIBITION OF ARACHIDONIC ACID-INDUCED EAR EDEMA, MAST-CELL STABILIZING AND FREE RADICAL EFFECT OF DICHLOROMETHANE CRUDE EXTRACTS FROM THE LEAVES OF LABISIA PUMILA

Authors

  • S.O.EKEUKU
  • P. N. OKECHUKWU

Abstract

Objective: To evaluate the ability of dichloromethane leave extract of labisia pumila (DELP) to  inhibit arachidonic acid - induced ear oedema, mast cell stabilizing effect and determination of DPPH,TPC, hydroxyl, nitric oxide and superoxide radical-scavenging activity of the extract.

Material and method: The leaf extract was prepared with dichloromethane filter and rotary evaporated and stored in desiccator. The effect of DELP on arachidonic acid - induced ear oedema, mast cell stabilizing was evaluated. DPPH,TPC, hydroxyl, nitric oxide and superoxide radical-scavenging activity of the extract was also studied.

Result: DELP was able to inhibit arachidonic acid induced ear edema in the mice. The concentration of DELP (mg/ml) needed for 50 percent  inhibition (IC50) of superoxide radicals, DPPH and NO were 1, 1.5, and 1.5 respectively. Total Phenolic Content was 25microgram GAE/mg.

 

Keywords— Anti-inflammation, antioxidant, ear-edema, Labisia pumila, mast-cell stabilization.

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Published

2013-10-01

How to Cite

S.O.EKEUKU, and P. N. OKECHUKWU. “INHIBITION OF ARACHIDONIC ACID-INDUCED EAR EDEMA, MAST-CELL STABILIZING AND FREE RADICAL EFFECT OF DICHLOROMETHANE CRUDE EXTRACTS FROM THE LEAVES OF LABISIA PUMILA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 4, Oct. 2013, pp. 93-95, https://innovareacademics.in/journals/index.php/ajpcr/article/view/499.

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