HEPATOPROTECTIVE ACTIVITY OF HYDRO-ALCOHOLIC EXTRACT OF WHOLE PLANT OF SOLANUM DULCAMARA L. AND NEPHROLEPIS CORDIFOLIA (L) C. PRESL AGAINST PARACETAMOL INDUCE HEPATOTOXICITY IN ALBINO RATS
Objective: To investigate the effect of hepatoprotective activity of hydro-alcoholic extract of Solanum dulcamara L. and Nephrolepis cordifolia (L)
C. Presl against paracetamol induced hepatotoxicity in rats.
Methods: Albino rats of either sex were divided into nine groups and treated for 7 days. Group I and II served as normal and toxic control, Group III
were treated with Silymarin (100 mg/kg), and Group IV to IX were treated with 200, 400 and 600 mg/kg hydro-alcoholic (70%v/v) extract of
S. dulcamara (HASD) and hydro-alcoholic (70%v/v) extract of N. cordifolia (HANC) respectively. The biochemical markers like serum glutamicpyruvic
transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), alkaline phosphatase (ALP), bilirubin (total and direct), total protein,
triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very low-density
lipoprotein cholesterol (VLDL-C). The in vivo antioxidant activity was determined by estimating the tissue levels of glutathione (GSH), superoxide
dismutase (SOD), catalase (CAT) and lipid peroxidation (LPO). Histopathology of liver was also carried out.
Results: The HASD and HANC (200, 400 and 600 mg/kg) produced significant effect by decreasing the activity or level of SGOT, SGPT, ALP, billirubin
and total protein, where decrease in total protein level in liver, TG, TC, LDL-C and VLDL-C levels and increase in the HDL-C. And decrease tissue LPO,
while it significantly increased the levels of tissue GSH, SOD and CAT in a dose-dependent manner.
Conclusion: From the present study it can be concluded that hydro-alcoholic extract of S. dulcamara L. and N. cordifolia (L) C. Presl whole plant
possesses hepatoprotective activity against paracetamol induced hepatotoxicity.
Keywords: Hepatoprotective, In-vivo antioxidant activity, Paracetamol, Solanum dulcamara L. and Nephrolepis cordifolia (L) C. Presl, Silymarin.
Linda DF, Sanjay K. Acetaminophen â€“ Induced Fulminant Liver Failure Liver Pathology. 1st ed. New York, USA: Published by Demos Medical Publishing; 2011. p. 217-9.
Handa SS, Sharma A, Chakraborti KK. Natural products and plants as liver protecting drugs. Fitoterapia 1986;57:307-51.
Dienstag JL, Isselbacher KJ. Toxic and drug-induced hepatitis. In: Braunwald E, et al, editors. Harrisonâ€™s Principles of Internal Medicine. 15th ed., Ch. 296. Vol. 2. The McGraw-Hill Companies, Inc.; 2001. p. 1737-42.
Piper DW, et al. Gastrointestinal and hepatic diseases. In: Speight TM, Holford NH, editors. Averyâ€™s Drug Treatment. 4th ed., Ch. 22. New Zealand: Adis International Limited; 1997. p. 937.
Khosla P, Bhanwra S, Singh J, Seth S, Srivastava RK. A study of hypoglycaemic effects of Azadirachta indica (Neem) in normaland alloxan diabetic rabbits. Indian J Physiol Pharmacol 2000;44(1):69-74.
Dass EE, Shah KK. Paracetamol and conventional antimalarial drugs induced hepatotoxicity and its protection by methionine in rats. Indian J Exp Biol 2000;38(11):1138-42.
Saraswathy SD, Suja V, Gurumurthy P, Shyamala Devi CS. Effect of liv.100 against antitubercular drugs (isoniazid, rifampicin and pyrazinamide) induced hepatotoxicity in rats. Indian J Pharmacol 1998;30:233-8.
Davies DM, editor. Text Book of Adverse Drug Reactions. 3rd ed. New York: Oxford University Press; 1985. p. 253-65.
Johnson-Fannin AM. Hepatitis: Viral and druginduced. In: Herfindal ET, Gourley DR, editors. Text Book of Therapeutics Drug and Disease Management. 6th ed., Ch. 27. USA: Williams and Wilkins; 1996. p. 543â€‘4.
Sharma SK, Ali M, Gupta J. Plants having hepatotprotective activity. Phytochem Pharmacol 2002;2:253-70.
Chaterjee TK. Medicinal plants with hepatoprotective properties. Herbal Options. 3rd ed. Calcutta: Books and Allied (P) Ltd.; 2000. p. 155.
Chattopadhyay RR. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: Part II. J Ethnopharmacol 2003;89(2-3):217-9.
OECD Guidelines for the Testing of Chemical. Acute Oral Toxicity â€“ Up and Down Procedure (UDP). 2008 Available from: http://www.iccvam.niehs.nih.gov/SuppDocs/FedDocs/OECD/OECDtg425.pdf. [Last cited on 2011 Sep 25].
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 1982;126:131-8.
Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990;186:407-21.
McComb RB, Bowers GN Jr. Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum. Clin Chem 1972;18(2):97-104.
Bergmeyer HU, HÃ¸rder M, Rej R. International Federation of Clinical Chemistry (IFCC) Scientific Committee, Analytical Section: Approved recommendation (1985) on IFCC methods for the measurement ofcatalytic concentration of enzymes. Part 3. IFCC method for alanine aminotransferase (L-alanine:2-oxoglutarate aminotransferase, EC 126.96.36.199). J Clin Chem Clin Biochem 1986;24(7):481-95.
Bergmeyer HU, HÃ¸rder M, Rej R. International Federation of Clinical Chemistry (IFCC) Scientific Committee, Analytical Section: Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 2. IFCC method for aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 188.8.131.52). J Clin Chem Clin Biochem 1986;24(7):497-510.
Ramachandra Setty S, Quereshi AA, Viswanath Swamy AH, Patil T, Prakash T, Prabhu K, et al. Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats. Fitoterapia 2007;78(7-8):451-4.
Janbaz KH, Saeed SA, Gilani AH. Protective effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents. Fitoterapia 2002;73(7-8):557-63.
Childs JF. Controlling orange decay. Indian J Chem 1946;38:82.
Shon YH, Nam KS. Protective effect of Moutan Cortex extract on acetaminophen-induced hepatotoxicity in mice. J Ethnopharmacol 2004;90(2-3):415-9.
Abraham P. Oxidative stress in paracetamol-induced pathogenesis: (I). Renal damage. Indian J Biochem Biophys 2005;42(1):59-62.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.