DIABETOGENIC INFLUENCE OF HYPERDIET AND CYCLOPHOSPHAMIDE ON THE NON OBESE DIABETIC (NOD) MOUSE.
Objective:Â The objective of the study is to evaluate the hyper diet and cyclophosphamide accelerated diabetes related metabolic changes in the male NOD mice stock.
Materials and Methods:Â Different groups of NOD male mice were fed with hyperdiet and injected intraperitoneally with cyclophosphamide single dose and multiple split doses. Hepatic Hexokinase
Glucose 6 phosphatase, Glucose-6-phosphate dehydrogenase, Glutathione reductase and
Glutathione s Transferase activities were determined.
Results:Â Significance differences showed in hyperglycemia in hyperdiet, cyclophosphamide single dose and cyclophosphamide multiple split dose treated animals. Glycosylated hemoglobin showed that there was a significant difference in all the three groups when compared with that of the control group. Whereas, hyperdiet treated and the cyclophosphamide multiple split dose treated animals showed significant similarity. The altered activities of hexokinase, glucose 6 phosphatase, and glucose 6 phosphate dehydrogenase in the liver of NOD male mice resulted in the hyperglycemic state of the treated animals.
Â Conclusion:Â Hyperdiet and cyclophosphamide treated animals showed significant hyperglycemia and also altered few enzyme activity of carbohydrate metabolism. Hence, the non diabetic NOD male mice treated with hyper diet and cyclophosphamide may be a useful model to test therapeutic approaches for amelioration of chronic diabetic complications in humans.
Key words: NOD mice, Diabetes, Hyperdiet, Cyclophosphamide
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