EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF ROOT OF COLEUS EDULIS AND ITS CORRELATION WITH IN VIVO HEPATOPROTECTIVE ACTIVITY IN PARACETAMOL INDUCED HEPATOTOXICITY IN RATS
Objective: People of Ethiopia chew the root of Coleus edulis (Lamiaceae) for the treatment of liver disorders. Since free radical mechanism are
reported to be involved in causing such disorders, the aqueous extract of root of C. edulis was screened for its antioxidant activity by in vitro and for
in vivo hepatoprotective activity against paracetamol (PCM) induced liver toxicity in rats.
Methods: The antioxidant activity was evaluated by estimating the lipid peroxidation inhibition, superoxide, and hydroxyl radical scavenging activities.
Hepatotoxicity was induced in rats by oral administration of 2 g/kg body weight of PCM for 3 days. Blood samples were collected and estimated the
alkaline phosphatase (ALP), glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and total bilirubin (T.BIL) in serum.
Results: The amount of extract and ascorbic acid needed for 50% scavenging of superoxide and hydroxyl radicals was found to be 65.56 Î¼g, 42.86 Î¼g,
930.47 Î¼g, and 817.06 Î¼g, respectively. The amount needed for 50% inhibition of lipid peroxide was 773.26 Î¼g (extract) and 807.70 Î¼g (ascorbic acid).
The preventive and curative treatment with the aqueous extract of root of C. edulis (300 mg/kg) found to be decrease the rise in serum ALP, serum
glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase and T.BIL levels due to PCM treatment. The results of the extract are
comparable with standard silymarin (100 mg/kg).
Conclusion: The lipid peroxidation inhibition activity coupled with free radical scavenging activity of the extract might be responsible for its protective
activity against PCM induced hepatotoxicity in rats.
Keywords: Coleus edulis, Hepatoprotective activity, Lipid peroxidation, Paracetamol.
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Table 9: Percentage protection of aqueous extract of C. edulis and silymarin against PCM induced liver damage in rats on 11th day (curative study)
Group E treated with PCM (2 g/kg)+C. edulis (300 mg/kg)
Group F treated with PCM (2 g/kg)+silymarin (100 mg/kg)
C. edulis: Colies edulis, PCM: Paracetamol, SGOT: Serum glutamate oxaloacetate transaminase, SGPT: Serum glutamate pyruvate transaminase, ALP: Alkaline phosphatase, T.BIL: Total bilirubin
Fig. 5: Average percentage protection produced by Colies edulis and silymarin against paracetamol induced hepatotoxicity on 11th day (curative study)
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