• Ashish Kumar Singha
  • Bhaskar Bhattacharjee
  • Ranjit Ghosh
  • Utpal Chandra De
  • Debasish Maiti Tripura University, India


Objective: The persent investigation is carried out to assess in vitro antimicrobial, antioxidant and anti-alphaglucosidase activity of the crude and fraction extracts of both leaf and fruits of the plant Dillenia pentagyna Roxb. (Dilleniaceae).

Methods: Extracts prepared from leaf and fruits by drying and fractionation process. Antibacterial activity assessed by disc diffusion and liquid culture method. Antioxidant activity was assayed by superoxide radical scavenging capability and by DPPH scavenging capability. By using the enzyme α-glucosidase and substrate p-nitrophenyl glucopyranoside (pNPG), the inhibitory activity of the extracts was assayed.

Results:  Crude extracts of fruits from both butanol fraction and chloroform fraction showed promising antibacterial activity in disc diffusion method through measuring inhibition zone. Both butanol and chloroform fraction showed a significant superoxide radical scavenging activity and using DPPH (1,1-diphenyl-2-picryl hydrazyl), butanol fraction extract of fruit showed a significant scavenging property. α-glucosidase (E.C No. enzyme activity is inhibited significantly by the leaf extract of the plant.

Conclusion: The results suggest that the crude plant extract contain some compounds which have antimicrobial activity, more potent antioxidant activity as well as α-glucosidase inhibitory activity.

Keywords: Dillenia pentagyna, antimicrobial, antioxidant, antidiabetic, superoxide, α-glucosidase, DPPH.


Download data is not yet available.


Uniyal SK, Singh KN, Jamwal P, Lal B Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalayan. J. Ethnobiol. Ethnomed 2006; 2: 1-14.

Srivastava J, Lambert J, Vietmeyer N Medicinal plants: An expanding role in development. World Bank Technical Paper 1996; 23: 320.

Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH Natural plant chemicals: Sources of Industrial and Medicinal materials. Science 1985; 228: 1154-1160.

Haque MF, Islam MN, Hossain M, Mohamad AU, Karim MF, Rahman MA Antimicrobial and Cytotoxic Activities of Dillenia pentagyna. Dhaka Univ. J. Pharm. Sci. 2008; 7 suppl 1: 103-105.

Dubey PC, Sikarwar RLS, Khanna KK, Tiwari AP Ethanobotany of Dillenia pentagyna Roxb. in Vindya Region of Madhya Pradesh, India. Natural Product Radiance 2009. 8 suppl 5: 546-548.

Sundarapandian SM, Swamy PS Litter production and leaf-litter decomposition of selected tree species in tropical forests at Kodayar in the Western Ghats, India. Forest Ecology and Management 1999; 123: 231-244.

Charimbu MK, Wagara IN, Otaye DO Antifungal activity of various crude plant extracts against Phaeoisariopsis griseola pathogenic on common bean. African Crop Science Conference Proceedings 2009; 9: 683–685.

Kumar D, Mallick S, Vedasiromoni JR, Pal BC Anti-leukemic activity of Dillenia indica L. fruit extract and quantification of betulinic acid by HPLC. Phytomedicine: International Journal of Phytotherapy & Phytopharmacology 2010; 17 Suppl 6: 431-435.

Duraipandiyan V, Ayyanar M, Ignacimuthu S Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complementary and Alternative Medicine 2006; 6: 35.

Christian K, Mobarak AM, Michael S, Can Eugen I, Stephan P, Torsten H Inhibitory activity of a standardized elderberry liquid extract against clinically-relevant human respiratory bacterial pathogens and influenza A and B viruses. BMC Complementary and Alternative Medicine 2011; 11: 16.

Mondal SK, Chakraborty G, Gupta M, Mazumdar UK In vitro Antioxidant Activity of Diospyros malabarica Kostel Bark. Indian J of Exp Biol 2006; 44: 39-44.

Ogunlana OE, Ogunlana OO. In vitro Assesment of the Free Radical Scavenging Activity of Psidium guajava. Res J of Agri and Bio Sciences 2008; 4(6): 666-671.

Cotelle A, Bernier JL, Catteau JP, Pommery J, Wallet JC Gaydou EM Antioxidant properties of hydroxyl-flavones. Free Radic Biol Med 1996; 20: 35.

Gupta RC, Sharma V, Sharma N, Kumar N, Singh B In vitro Antioxidant Activity from Leaves of Oroxylum indicum (L.) Vent. -A North Indian Highly Threatened and Vulnerable Medicinal Plant. J of Pharm Res 2008; 1 Suppl 1: 65-72.

Singha AK, Bhattacharjee B, Das N, Dinda B, Maiti D Ichnocarpus frutescens (linn) -a plant with different biological activities. Asian J Pharm Clin Res 2013; 6(Suppl 1): 74-77.

Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 1998; 37: 837.

Sreejamole KL, Radhakrishnan CK. Antioxidant and cytotoxic activities of ethyl acetate extract of the indian green mussel Perna viridis. Asian J Pharm Clin Res 2013; 6 Suppl 3: 197-201.

Pari L, Uma MJ Hypoglycemic effect of Musa sapientum L. in alloxan-induced diabetic rats. Journal of Ethnopharmacology 1999; 68: 321–325.

Piedrola G, Novo E, Escober F, Garcia-Robles R White blood cell count and insulin resistance in patients with coronary artery disease. Annual Endocrinology 2001; 62: 7–10.

Yin J, Heo S-II, Wang MH Antioxidant and Antidiabetic Activities of Extracts from Cirsium japonicum roots. Nutrition Research and Practice 2008; 2 Suppl 4: 247-251.

N Festjens1, M Kalai1,2, J Smet3, A Meeus1, R Van Coster3, X Saelens1,4 and P Vandenabeele*,1 Butylated hydroxyanisole is more than a reactive oxygen species scavenger; Cell Death and Differentiation 2006; 13: 166–169



How to Cite

Singha, A. K., B. Bhattacharjee, R. Ghosh, U. Chandra De, and D. Maiti. “ANTIBACTERIAL, ANTI-ALPHA GLUCOSIDASE AND ANTIOXIDANT PROPERTIES OF DILLENIA PENTAGYNA ROXB. (DILLENIACEAE)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 4, Oct. 2013, pp. 173-7,