PHYTOCHEMICAL SCREENING AND GC-MS PROFILING IN THE LEAVES OF SOLANUM INCANUM L

Authors

  • Sundar S Sathyabama university
  • Justin Koil Pillai Y

Abstract

chromatograph-mass spectrometer (GC-MS) method.
Methods: The phytochemicals present in the petroleum ether, methanol, ethanol extract of S. incanum L. leaves was investigated by using GC Shimadzu
QP2010 system and GC interfaced to a MS equipped with Elite-1 fused silica capillary column. The compounds were separated by using helium as the
carrier gas at a constant flow rate of 1.51 ml/minutes. Software adopted to handle mass spectra and chromatograms were GC-MS solution ver. 2.53.
Results: Preliminary studies showed the presence of carbohydrates, coumarins, flavonoid, phenol, steroids, and phytosteroids. In the GC-MS analysis
of petroleum ether, ethanol, and methanol extract of S. incanum L. reported the presence of 12 components. The major chemical constituents are
docosahexaenoic acid, 1,2,3 propanetriyl ether (retention time [RT] 9.18), ergoline-8-carboxylic acid, 10-methoxy-6-methyl-methyl ester, (8a) (RT
10.63), ascorbic acid 2,6-dihexadecanoate (RT 11.9), propanoic acid, 2-(3-acetoxy-4,4, 14 trimethylandrost-8-en-17-yl) (RT 12.44).
Conclusion: This type of GC-MS analysis is the first step towards understanding the nature of active principles in this plant. The isolation of individual
bioactive components and their biological activity are necessary for future studies and help to find new drugs.

Keywords: Gas chromatograph-mass spectrometer profiling, Phyto components, Solanum incanum L., Ascorbic acid 2,6-dihexadecanoate.

References

Azaizeh H, Fulder S, Khalil K, Said O. Ethnobotanical knowledge

of local Arab practitioners in the Middle Eastern region. Fitoterapia

;74(1-2):98-108.

Beaman-Mbaya V, Muhammed SI. Antibiotic action of Solanum

incanum Linnaeus. Antimicrob Agents Chemother 1976;9(6):920-4.

Chevallier A. The Encyclopedia of Medicinal Plants. London: Dorling

Kindersley; 1996.

Britto SJ, Senthinkumar S. Antimicrobial activities of Solanum incanum

leaf extract. Asian J Microbiol Biotechnol Environ Sci 2001;3(1-

:65-6.

Lin CN, Lu CM, Cheng MK, Gan KH, Won SJ. The cytotoxic principles

of Solanum incanum. J Nat Prod 1990;53(2):513-6.

Kupchan SM, Barboutis SJ, Knox JR, Cam CA. Beta-solamarine:

Tumor inhibitor isolated from Solanum dulcamara. Science

;150(3705):1827-8.

Mukherjee PK. Quality Control of Herbal Drugs: An Approach

of Evaluation of Botanicals. New Delhi: Business Horizons

Pharmaceuticals Publishers; 2002.

Khandelwal KR. Practical Pharmacognosy Technique and Experiments.

rd ed. Vol. 15-29. Pune: Nirali Prakashan; 2005. p. 149-56.

Kokate CK. Practical Pharmacognosy. Delhi: Vallabh Prakashan; 2000.

p. 107-11.

Arunkumar S, Muthuselvam M. Analysis of phytochemical constituents

and antimicrobial activities of Aloe vera L. against clinical pathogens.

World J Agric Sci 2009;5(5):572-6.

Patel DK, Patel K, Duraiswamy B, Dhanabal SP. Phytochemical

analysis and standardization of Strychnos nux-vomica extract through

HPTLC techniques. Asian Pac J Trop Dis 2012;2(1):S56-60.

Published

2015-05-01

How to Cite

Sundar S, and J. K. Pillai Y. “PHYTOCHEMICAL SCREENING AND GC-MS PROFILING IN THE LEAVES OF SOLANUM INCANUM L”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 3, May 2015, pp. 179-88, https://innovareacademics.in/journals/index.php/ajpcr/article/view/5246.

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