COMPARATIVE INSIGHTS INTO ULTRAVIOLET-B RADIATION-INDUCED BIOCHEMICAL MODULATIONS IN SENNA AURICULATA: A GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING STUDY

SELVAKUMAR MURUGAN; SHANTHI NATARAJAN; SANGEETHA SOUNDARARAJAN; SATHISH KUMAR BOOBALAN; MURUGESAN SUBBIAH

doi:10.22159/ajpcr.2025v18i2.53568

Authors

SELVAKUMAR MURUGAN Department of Botany, Unit of Plant Stress Physiology, Pachaiyappa’s College, Affiliated to University of Madras, Chennai, Tamil Nadu, India.
SHANTHI NATARAJAN Department of Botany, Unit of Plant Stress Physiology, Pachaiyappa’s College, Affiliated to University of Madras, Chennai, Tamil Nadu, India.
SANGEETHA SOUNDARARAJAN Department of Zoology, Pachaiyappa’s College, Affiliated to University of Madras, Chennai, Tamil Nadu, India
SATHISH KUMAR BOOBALAN Department of Botany, School of Science, Tamil Nadu Open University, Chennai, Tamil Nadu, India.
MURUGESAN SUBBIAH Department of Botany, Pachaiyappa’s College, Affiliated to University of Madras, Chennai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2025v18i2.53568

Keywords:

Ultraviolet-B, Gas chromatography-mass spectrometry, Secondary metabolites, Antioxidant activity, Anticancer properties, Antibacterial properties

Abstract

Objectives: This study explores the transformative effects of ultraviolet B (UV-B) radiation on the phytochemical profile of Senna auriculata leaves, a plant known for its medicinally properties.

Methods: Utilizing gas chromatography-mass spectrometry, we systematically compared the phytochemical profiles of methanolic extracts from untreated leaves (SKC) and those exposed to UV-B radiation (SKT).

Results: The analysis revealed a total of 59 compounds in the untreated leaves (SKC) and 50 in the UV-B exposed leaves (SKT). Among these, 36 metabolites were common to both samples, while 23 were unique to SKC, and 14 were exclusive to SKT. Notable compounds induced by UV-B radiation included Cystamine, 2-Methyl-6-(5-methyl-2-thiazolin-2-ylamino) pyridine, and 1,2-Benzisothiazol-3-amine tbdms. These compounds exhibited significant bioactivities, demonstrating antioxidant, anticancer, and antibacterial properties.

Conclusion: The findings highlight the role of UV-B radiation as a modulator of secondary metabolism, reshaping the phytochemical profile of S. auriculata to enhance its adaptive resilience and therapeutic potential. This study sheds light on the complex interaction between environmental stressors and phytochemistry, providing valuable insights into how controlled UV-B exposure can optimize the medicinal properties of plants.

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