• SivaramaKrishnan Rajaraman Department of Biomedical Engineering, SSN College of Engineering
  • Guruprakash Subbiahdoss Associate Professor, SSN College of Engineering
  • Gnanaprakash Dhakshinamoorthy Associate Professor, SSN College of Engineering
  • Subashini Rajakannu SSN College of Engineering


Objective: The objective of this work is to evaluate the performance of OS extract as a coating on biomaterial surfaces in preventing bacterial adhesion
and biofilm growth, as an effective measure to combat Biomaterial associated infections.
Methods: Here, we have incorporated the extract from a medicinal plant as a coating to biomaterial surfaces in order to prevent bacterial adhesion
and biofilm growth. To this end, Ocimum sanctum (OS) oil extract is coated on biomaterials (polymethyl methacrylate and polystyrene) and bacteria
such as Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were allowed to adhere and grow for 1 hr, 3 hrs and 24 hrs.
Results: A significant reduction (p<0.01) in number of adherent bacteria on OS extract coated surfaces compared to bare surfaces was observed at
all-time points. The zone of inhibition of OS extract was observed for all the three bacteria and maximum inhibition was observed for P. aeruginosa
(30 mm diameter) compared to S. aureus (25 mm diameter) and E. coli (28 mm diameter).
Conclusion: Thus, OS oil extract could be a promising coating for reduction of bacterial adhesion and biofilm formation.

Keywords: Antibacterial coating, Bacterial adhesion, Biofilm, Biomaterial, Biomaterials-associated infection, Ocimum sanctum.


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Author Biographies

SivaramaKrishnan Rajaraman, Department of Biomedical Engineering, SSN College of Engineering

Assistant Professor,

Department of Biomedical Engineering

Guruprakash Subbiahdoss, Associate Professor, SSN College of Engineering

Associate Professor

Gnanaprakash Dhakshinamoorthy, Associate Professor, SSN College of Engineering

Associate Professor

Subashini Rajakannu, SSN College of Engineering

Assistant Professor


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How to Cite

Rajaraman, S., G. Subbiahdoss, G. Dhakshinamoorthy, and S. Rajakannu. “OCIMUM SANCTUM EXTRACT COATING ON BIOMATERIAL SURFACES TO PREVENT BACTERIAL ADHESION AND BIOFILM GROWTH”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 3, May 2015, pp. 229-33, https://innovareacademics.in/journals/index.php/ajpcr/article/view/5533.



Original Article(s)