• Rahul Shivaji Adnaik
  • Shrinivas Krishna Mohite


Objective: The present study was aimed to evaluate the neuroprotective effects of Lagenaria vulgaris seed oil (LVSO) in hypoxic neurotoxicity induced
neurodegeneration in mice.
Methods: Hypoxia was induced by providing sodium nitrite (5 ml/kg) in drinking water to mice for 14 days and mice were evaluated for elevated
plus maze test. On 15th day, mice were sacrificed and brain was isolated for estimation of non-enzymatic antioxidants (glutathione [GSH]), enzymatic
antioxidants superoxide dismutase (SOD), catalase (CAT), total protein (TP) in the brain tissue, and histopathological study.
Results: Treatment with LVSO significantly prevented raise in levels of oxidant enzymes. CAT, GSH, SOD, and TP content have significantly restored in
LVSO treated groups whereas toxic group has shown a significant decrease in levels compare to control group. LVSO (0.5 mg/kg) has shown maximum
protection. The observed results leading to neurodegeneration is also confirmed by the histopathological differences between treatment and hypoxic
Conclusion: LVSO ameliorated antihypoxic effects induced by sodium nitrite and this effect may be mediated by antioxidant mechanism, thereby
enhancing neuroprotection.
Keywords: Neuroprotective, Antioxidants, Hypoxia, Seed oil.


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Fig. 4: Histopathological observations of brain in hypoxic neurotoxicity in mice, (a) Control group, (b) hypoxic group, (c) Lagenaria vulgaris seed oil (LVSO) (0.25 g/kg), (d) LVSO (0.5 g/kg)
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How to Cite
Adnaik, R. S., and S. Krishna Mohite. “NEUROPROTECTIVE EVALUATION OF LAGENARIA VULGARIS EXTRACT HYPOXIC NEUROTOXICITY INDUCED RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 4, July 2015, pp. 121-4,
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