NEUROPROTECTIVE EVALUATION OF LAGENARIA VULGARIS EXTRACT HYPOXIC NEUROTOXICITY INDUCED RATS
Objective: The present study was aimed to evaluate the neuroprotective effects of Lagenaria vulgaris seed oil (LVSO) in hypoxic neurotoxicity induced
neurodegeneration in mice.
Methods: Hypoxia was induced by providing sodium nitrite (5 ml/kg) in drinking water to mice for 14 days and mice were evaluated for elevated
plus maze test. On 15th day, mice were sacrificed and brain was isolated for estimation of non-enzymatic antioxidants (glutathione [GSH]), enzymatic
antioxidants superoxide dismutase (SOD), catalase (CAT), total protein (TP) in the brain tissue, and histopathological study.
Results: Treatment with LVSO significantly prevented raise in levels of oxidant enzymes. CAT, GSH, SOD, and TP content have significantly restored in
LVSO treated groups whereas toxic group has shown a significant decrease in levels compare to control group. LVSO (0.5 mg/kg) has shown maximum
protection. The observed results leading to neurodegeneration is also confirmed by the histopathological differences between treatment and hypoxic
Conclusion: LVSO ameliorated antihypoxic effects induced by sodium nitrite and this effect may be mediated by antioxidant mechanism, thereby
Keywords: Neuroprotective, Antioxidants, Hypoxia, Seed oil.
1. Halliwell B, Gutteridge JM, Cross CE. Free radicals, antioxidants, and human disease: Where are we now? J Lab Clin Med 1992;119(6):598â€‘620.
2. Markesbery WR. Oxidative stress hypothesis in Alzheimerâ€™s disease. Free Radic Biol Med 1997;23(1):134-47.
3. Floyd RA. Antioxidants, oxidative stress, and degenerative neurological disorders. Proc Soc Exp Biol Med 1999;222(3):236-45.
4. Gilgun-Sherki Y, Melamed E, Offen D. Oxidative stress induced-neurodegenerative diseases: The need for antioxidants that penetrate the blood brain barrier. Neuropharmacology 2001;40(8):959-75.
5. Fahn S, Sulzer D. Neurodegeneration and neuroprotection in Parkinson disease. NeuroRx 2004;1(1):139-54.
Fig. 4: Histopathological observations of brain in hypoxic neurotoxicity in mice, (a) Control group, (b) hypoxic group, (c) Lagenaria vulgaris seed oil (LVSO) (0.25 g/kg), (d) LVSO (0.5 g/kg)
6. LÃ¶hle M, Reichmann H. Clinical neuroprotection in Parkinsonâ€™s disease â€“ Still waiting for the breakthrough. J Neurol Sci 2010;289(1â€‘2):104-14.
7. Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Dehradun, Uttaranchal, India: Oriental Enterprises; 2003.
8. Prajapati RP, Kalariya M, Parmar SK, Sheth NR. Phytochemical and pharmacological review of Lagenaria sicereria. J Ayurveda Integr Med 2010;1(4):266-72.
9. Deshpande JR, Choudhari AA, Mishra MR, Meghre VS, Wadodkar SG, Dorle AK. Beneficial effects of Lagenaria siceraria (Mol.) Standley fruit epicarp in animal models. Indian J Exp Biol 2008;46(4):234-42.
10. Deshpande JR, Mishra MR, Meghre VS, Wadodkar SG, Dorle AK. Antioxidant activity of Lagenaria siceraria (Mol) stand. Fruit. Nat Prod Radiance 2007;6(2):127-30.
11. Ghule BV, Ghante MH, Saoji AN, Yeole PG. Hypolipidemic and antihyperlipidemic effects of Lagenaria siceraria (Mol.) fruit extracts. Indian J Exp Biol 2006;44(11):905-9.
12. Ghule BV, Ghante MH, Upganlawar AB, Yeole PG. Analgesic and anti-inflammatory activities of L. siceraria (Mol) stand. fruit juice extract in rats and mice. Pharmacog Mag 2006;2:232.
13. Shirwaikar A, Sreenivasan KK. Chemical investigation and antihepatotoxic activity of the fruits of Lagenaria siceraria. Indian J Pharm Sci 1996;58(5):197.
14. Kokate CK. Practical Pharmacognosy. 5th ed. New Delhi: Vallabh Prakashan; 1991.
15. Greenamyre JT. The role of glutamate in neurotransmission and in neurologic disease. Arch Neurol 1986;43(10):1058-63.
16. Homola A, Zoremba N, Slais K, Kuhlen R, SykovÃ¡ E. Changes in diffusion parameters, energy-related metabolites and glutamate in the rat cortex after transient hypoxia/ischemia. Neurosci Lett 2006 14;404(1â€‘2):137-42.
17. Kumar P, Kumar A. Protective role of sertraline against 3-nitropropionic acid-induced cognitive dysfunction and redox ratio in striatum, cortex and hippocampus of rat brain. Indian J Exp Biol 2009;47(9):715-22.
18. Kumar S, Misra UK, Kalita J, Khanna VK, Khan MY. Imbalance in oxidant/antioxidant system in different brain regions of rat after the infection of Japanese encephalitis virus. Neurochem Int 2009;55(7):648â€‘54.
19. Dandu AM, Inamdar NM. Evaluation of beneficial effects of antioxidant properties of aqueous leaf extract of Andrographis paniculata in STZ-induced diabetes. Pak J Pharm Sci 2009;22(1):49-52.
20. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):35l-8.
21. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82(1):70-7.
22. Dash DK, Yeligar VC, Nayak SS, Ghosh T, Rajalingam D, et al. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R. Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res 2007;6(3):755-65.
23. Jain A, Flora SJ. Dose related effects of nicotine on oxidative injury in young, adult and old rats. J Environ Biol 2012;33(2):233-8.
24. Young DS, Pestaner LC, Gibberman V. Effects of drugs on clinical laboratory tests. Clin Chem 1975;21(5):1D-432.
25. Friedman RB, Anderson RE, Entine SM, Hirshberg SB. Effects of diseases on clinical laboratory tests. Clin Chem 1980;26 4 Suppl:1D-476.
26. Chandrashekhar VM, Ranpariya VL, Ganapaty S, Parashar A, Muchandi AA. Neuroprotective activity of Matricaria recutita Linn against global model of ischemia in rats. J Ethnopharmacol 2010;127:645-51.
27. Tariq M, Khan HA, Elfaki I, Al Deeb S, Al Moutaery K. Neuroprotective effect of nicotine against 3-nitropropionic acid (3-NP)-induced experimental Huntingtonâ€™s disease in rats. Brain Res Bull 2005;67(1â€‘2):161-8.
28. TÃºnez I, Tasset I, PÃ©rez-De La Cruz V, SantamarÃa A. 3-Nitropropionic acid as a tool to study the mechanisms involved in Huntingtonâ€™s disease: Past, present and future. Molecules 2010;15:878-916.
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